Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes
The in vitro effect of okadaic acid on basal phorbol 12-myristate 13-acetate (PMA)-, and cyclic adenosine monophosphate (cAMP)-stimulated Mg(2+)-dependent Ca(2+)-adenosine triphosphatase (ATPase) activity in synaptosomal membranes isolated from rat brain cortex and cerebellum was investigated. The b...
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| Veröffentlicht in: | Cellular signalling 1996-09, Vol.8 (6), p.443 |
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| creator | Zylinska, L Gromadzinska, E Lachowicz, L |
| description | The in vitro effect of okadaic acid on basal phorbol 12-myristate 13-acetate (PMA)-, and cyclic adenosine monophosphate (cAMP)-stimulated Mg(2+)-dependent Ca(2+)-adenosine triphosphatase (ATPase) activity in synaptosomal membranes isolated from rat brain cortex and cerebellum was investigated. The basal activity was enhanced by okadaic acid in both examined regions. This inhibitor differed in the regulation of Mg2+, Ca(2+)-ATPase activity in PMA- and cAMP-incubated membranes. Stimulation by calmodulin (CaM) of basal Mg2+, Ca(2+)-ATPase activity declined in cortex and cerebellum after treatment with okadaic acid. The presence of PMA or cAMP decreases the stimulatory effect of CaM. These results suggest that Mg2+, Ca(2+)-ATPase activity in the rat-brain synaptosomal membrane may be regulated in vitro by dephosphorylation processes. |
| doi_str_mv | 10.1016/S0898-6568(96)00080-0 |
| format | Article |
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The basal activity was enhanced by okadaic acid in both examined regions. This inhibitor differed in the regulation of Mg2+, Ca(2+)-ATPase activity in PMA- and cAMP-incubated membranes. Stimulation by calmodulin (CaM) of basal Mg2+, Ca(2+)-ATPase activity declined in cortex and cerebellum after treatment with okadaic acid. The presence of PMA or cAMP decreases the stimulatory effect of CaM. These results suggest that Mg2+, Ca(2+)-ATPase activity in the rat-brain synaptosomal membrane may be regulated in vitro by dephosphorylation processes.</description><identifier>ISSN: 0898-6568</identifier><identifier>DOI: 10.1016/S0898-6568(96)00080-0</identifier><identifier>PMID: 8958447</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Ca(2+) Mg(2+)-ATPase - metabolism ; Calmodulin - pharmacology ; Cerebellum - enzymology ; Cerebral Cortex - enzymology ; Cyclic AMP - pharmacology ; Enzyme Inhibitors - pharmacology ; Intracellular Membranes - enzymology ; Okadaic Acid - pharmacology ; Phosphoprotein Phosphatases - antagonists & inhibitors ; Phosphorylation ; Rats ; Rats, Wistar ; Synaptosomes - enzymology ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>Cellular signalling, 1996-09, Vol.8 (6), p.443</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8958447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zylinska, L</creatorcontrib><creatorcontrib>Gromadzinska, E</creatorcontrib><creatorcontrib>Lachowicz, L</creatorcontrib><title>Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>The in vitro effect of okadaic acid on basal phorbol 12-myristate 13-acetate (PMA)-, and cyclic adenosine monophosphate (cAMP)-stimulated Mg(2+)-dependent Ca(2+)-adenosine triphosphatase (ATPase) activity in synaptosomal membranes isolated from rat brain cortex and cerebellum was investigated. The basal activity was enhanced by okadaic acid in both examined regions. This inhibitor differed in the regulation of Mg2+, Ca(2+)-ATPase activity in PMA- and cAMP-incubated membranes. Stimulation by calmodulin (CaM) of basal Mg2+, Ca(2+)-ATPase activity declined in cortex and cerebellum after treatment with okadaic acid. The presence of PMA or cAMP decreases the stimulatory effect of CaM. These results suggest that Mg2+, Ca(2+)-ATPase activity in the rat-brain synaptosomal membrane may be regulated in vitro by dephosphorylation processes.</description><subject>Animals</subject><subject>Ca(2+) Mg(2+)-ATPase - metabolism</subject><subject>Calmodulin - pharmacology</subject><subject>Cerebellum - enzymology</subject><subject>Cerebral Cortex - enzymology</subject><subject>Cyclic AMP - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Intracellular Membranes - enzymology</subject><subject>Okadaic Acid - pharmacology</subject><subject>Phosphoprotein Phosphatases - antagonists & inhibitors</subject><subject>Phosphorylation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Synaptosomes - enzymology</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>0898-6568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKAzEYhbNQaq0-QiHLFh39k-mkM8tSvEGlgnVd_txKdGYyJKnQJ_C1nWJxdTgXvsUhZMzgjgET9-9QVmUmClFOKjEFgBIyOCPD__iCXMb4CcAKEHxABmVVlLPZfEh-1l-o0SmKymmKkSLtgpeGWh9oMLt9jcn5lrqWfrsUPPWWvu4m_GZ6S5d41GyxecNoekBy_eRwnAZMVPmQnMKaYqupMsFIU9cYaDy02CUffdN3jWlkwNbEK3JusY7m-qQj8vH4sFk-Z6v108tysco6xlnKrM6L3AosrJJCcMYFyEJzXYIEo7i2Fbcqn3PWG1kJU1UzrrTEXIvCzBXkIzL-43Z72Ri97YJrMBy2p0PyX3cGYkY</recordid><startdate>199609</startdate><enddate>199609</enddate><creator>Zylinska, L</creator><creator>Gromadzinska, E</creator><creator>Lachowicz, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199609</creationdate><title>Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes</title><author>Zylinska, L ; Gromadzinska, E ; Lachowicz, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p121t-fd353f6a5fcb6621260b5d2d80b0ec2df92fc3721ec2b96e9942cdba3d65e7c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Ca(2+) Mg(2+)-ATPase - metabolism</topic><topic>Calmodulin - pharmacology</topic><topic>Cerebellum - enzymology</topic><topic>Cerebral Cortex - enzymology</topic><topic>Cyclic AMP - pharmacology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Intracellular Membranes - enzymology</topic><topic>Okadaic Acid - pharmacology</topic><topic>Phosphoprotein Phosphatases - antagonists & inhibitors</topic><topic>Phosphorylation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Synaptosomes - enzymology</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zylinska, L</creatorcontrib><creatorcontrib>Gromadzinska, E</creatorcontrib><creatorcontrib>Lachowicz, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zylinska, L</au><au>Gromadzinska, E</au><au>Lachowicz, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>1996-09</date><risdate>1996</risdate><volume>8</volume><issue>6</issue><spage>443</spage><pages>443-</pages><issn>0898-6568</issn><abstract>The in vitro effect of okadaic acid on basal phorbol 12-myristate 13-acetate (PMA)-, and cyclic adenosine monophosphate (cAMP)-stimulated Mg(2+)-dependent Ca(2+)-adenosine triphosphatase (ATPase) activity in synaptosomal membranes isolated from rat brain cortex and cerebellum was investigated. The basal activity was enhanced by okadaic acid in both examined regions. This inhibitor differed in the regulation of Mg2+, Ca(2+)-ATPase activity in PMA- and cAMP-incubated membranes. Stimulation by calmodulin (CaM) of basal Mg2+, Ca(2+)-ATPase activity declined in cortex and cerebellum after treatment with okadaic acid. The presence of PMA or cAMP decreases the stimulatory effect of CaM. These results suggest that Mg2+, Ca(2+)-ATPase activity in the rat-brain synaptosomal membrane may be regulated in vitro by dephosphorylation processes.</abstract><cop>England</cop><pmid>8958447</pmid><doi>10.1016/S0898-6568(96)00080-0</doi></addata></record> |
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| subjects | Animals Ca(2+) Mg(2+)-ATPase - metabolism Calmodulin - pharmacology Cerebellum - enzymology Cerebral Cortex - enzymology Cyclic AMP - pharmacology Enzyme Inhibitors - pharmacology Intracellular Membranes - enzymology Okadaic Acid - pharmacology Phosphoprotein Phosphatases - antagonists & inhibitors Phosphorylation Rats Rats, Wistar Synaptosomes - enzymology Tetradecanoylphorbol Acetate - pharmacology |
| title | Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes |
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