Granulocyte colony-stimulating factor administration reverses cadmium-associated inhibition of hepatocyte regeneration
To document whether the administration of granulocyte colony-stimulating factor (G-CSF) enhances the impaired regenerative response of hepatocytes to partial hepatectomy (PH), in cadmium-pretreated partially hepatectomized rats. Rats were injected intraperioneally with 2.5 mg CdCl2/kg body weight, 2...
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| Veröffentlicht in: | European journal of gastroenterology & hepatology 1996-08, Vol.8 (8), p.805-809 |
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| container_title | European journal of gastroenterology & hepatology |
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| creator | THEOCHARIS, S. E MARGELI, A. P GOUTAS, N. D HORTI, M. G KARKANTARIS, C. S KITTAS, C. N |
| description | To document whether the administration of granulocyte colony-stimulating factor (G-CSF) enhances the impaired regenerative response of hepatocytes to partial hepatectomy (PH), in cadmium-pretreated partially hepatectomized rats.
Rats were injected intraperioneally with 2.5 mg CdCl2/kg body weight, 24h before PH. G-CSF (1500 or 150 micrograms/kg body weight) or saline was administered intraperitoneally in cadmium-pretreated partially hepatectomized rats at the same time as PH. The liver regenerative process was estimated 24h after PH. [3H] thymidine incorporation into liver DNA, liver thymidine kinase (TK) activity, mitotic index and proliferating cell nuclear antigen (PCNA) immunostaining were used as indices of hepatocyte proliferation.
G-CSF administration in cadmium-pretreated partially hepatectomized rats restored the suppressed DNA biosynthesis and TK activity (P < 0.001), to levels similar to those found in rats that were partially hepatectomized only. The mitotic index and the percentage of PCNA positive nuclei in hepatocytes were also enhanced in G-CSF administered cadmium-pretreated partially hepatectomized groups of rats.
The administration of G-CSF triggers events that restore the impaired liver regeneration in this model of reduced hepatocyte proliferation. |
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Rats were injected intraperioneally with 2.5 mg CdCl2/kg body weight, 24h before PH. G-CSF (1500 or 150 micrograms/kg body weight) or saline was administered intraperitoneally in cadmium-pretreated partially hepatectomized rats at the same time as PH. The liver regenerative process was estimated 24h after PH. [3H] thymidine incorporation into liver DNA, liver thymidine kinase (TK) activity, mitotic index and proliferating cell nuclear antigen (PCNA) immunostaining were used as indices of hepatocyte proliferation.
G-CSF administration in cadmium-pretreated partially hepatectomized rats restored the suppressed DNA biosynthesis and TK activity (P < 0.001), to levels similar to those found in rats that were partially hepatectomized only. The mitotic index and the percentage of PCNA positive nuclei in hepatocytes were also enhanced in G-CSF administered cadmium-pretreated partially hepatectomized groups of rats.
The administration of G-CSF triggers events that restore the impaired liver regeneration in this model of reduced hepatocyte proliferation.</description><identifier>ISSN: 0954-691X</identifier><identifier>EISSN: 1473-5687</identifier><identifier>PMID: 8864679</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Animals ; Biological and medical sciences ; Cadmium - toxicity ; Disease Models, Animal ; Granulocyte Colony-Stimulating Factor - pharmacology ; Hepatectomy ; Liver - cytology ; Liver - drug effects ; Liver - physiology ; Liver Regeneration - drug effects ; Liver, biliary tract, pancreas, portal circulation, spleen ; Male ; Medical sciences ; Mitotic Index ; Rats ; Rats, Wistar ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the digestive system</subject><ispartof>European journal of gastroenterology & hepatology, 1996-08, Vol.8 (8), p.805-809</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3191176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8864679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>THEOCHARIS, S. E</creatorcontrib><creatorcontrib>MARGELI, A. P</creatorcontrib><creatorcontrib>GOUTAS, N. D</creatorcontrib><creatorcontrib>HORTI, M. G</creatorcontrib><creatorcontrib>KARKANTARIS, C. S</creatorcontrib><creatorcontrib>KITTAS, C. N</creatorcontrib><title>Granulocyte colony-stimulating factor administration reverses cadmium-associated inhibition of hepatocyte regeneration</title><title>European journal of gastroenterology & hepatology</title><addtitle>Eur J Gastroenterol Hepatol</addtitle><description>To document whether the administration of granulocyte colony-stimulating factor (G-CSF) enhances the impaired regenerative response of hepatocytes to partial hepatectomy (PH), in cadmium-pretreated partially hepatectomized rats.
Rats were injected intraperioneally with 2.5 mg CdCl2/kg body weight, 24h before PH. G-CSF (1500 or 150 micrograms/kg body weight) or saline was administered intraperitoneally in cadmium-pretreated partially hepatectomized rats at the same time as PH. The liver regenerative process was estimated 24h after PH. [3H] thymidine incorporation into liver DNA, liver thymidine kinase (TK) activity, mitotic index and proliferating cell nuclear antigen (PCNA) immunostaining were used as indices of hepatocyte proliferation.
G-CSF administration in cadmium-pretreated partially hepatectomized rats restored the suppressed DNA biosynthesis and TK activity (P < 0.001), to levels similar to those found in rats that were partially hepatectomized only. The mitotic index and the percentage of PCNA positive nuclei in hepatocytes were also enhanced in G-CSF administered cadmium-pretreated partially hepatectomized groups of rats.
The administration of G-CSF triggers events that restore the impaired liver regeneration in this model of reduced hepatocyte proliferation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cadmium - toxicity</subject><subject>Disease Models, Animal</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Hepatectomy</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Liver - physiology</subject><subject>Liver Regeneration - drug effects</subject><subject>Liver, biliary tract, pancreas, portal circulation, spleen</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mitotic Index</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the digestive system</subject><issn>0954-691X</issn><issn>1473-5687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLAzEUhYMotVZ_gpCF20AeM3kspWgtFNwouCuZzE0bmZkMSabQf2-1xdWF8x0-OPcKzVmlBKmlVtdoTk1dEWnY1y26y_mbUqYEUzM001pWUpk5OqySHaYuumMB7GIXhyPJJfRTZ0sYdthbV2LCtu3DEHJJpzQOOMEBUoaM3S-YemJzji7YAi0Owz404a8WPd7DaMvZnmAHA5wN9-jG2y7Dw-Uu0Ofry8fyjWzeV-vl84aMXNSFNIYzIVvOvLC6AcuF0eA491B7YZzk2gtuJVUNp8YorRpFhaPUqRpoRSuxQI9n7zg1PbTbMYXepuP2sv_Eny7cZmc7f3qGC_m_JphhTEnxA1wBaDo</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>THEOCHARIS, S. E</creator><creator>MARGELI, A. P</creator><creator>GOUTAS, N. D</creator><creator>HORTI, M. G</creator><creator>KARKANTARIS, C. S</creator><creator>KITTAS, C. N</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19960801</creationdate><title>Granulocyte colony-stimulating factor administration reverses cadmium-associated inhibition of hepatocyte regeneration</title><author>THEOCHARIS, S. E ; MARGELI, A. P ; GOUTAS, N. D ; HORTI, M. G ; KARKANTARIS, C. S ; KITTAS, C. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-b92136d21f3a8bea2398ec22fe5f39c628f32a607b2099787b703c00c75e04043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cadmium - toxicity</topic><topic>Disease Models, Animal</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Hepatectomy</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Liver - physiology</topic><topic>Liver Regeneration - drug effects</topic><topic>Liver, biliary tract, pancreas, portal circulation, spleen</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mitotic Index</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THEOCHARIS, S. E</creatorcontrib><creatorcontrib>MARGELI, A. P</creatorcontrib><creatorcontrib>GOUTAS, N. D</creatorcontrib><creatorcontrib>HORTI, M. G</creatorcontrib><creatorcontrib>KARKANTARIS, C. S</creatorcontrib><creatorcontrib>KITTAS, C. N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>European journal of gastroenterology & hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THEOCHARIS, S. E</au><au>MARGELI, A. P</au><au>GOUTAS, N. D</au><au>HORTI, M. G</au><au>KARKANTARIS, C. S</au><au>KITTAS, C. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Granulocyte colony-stimulating factor administration reverses cadmium-associated inhibition of hepatocyte regeneration</atitle><jtitle>European journal of gastroenterology & hepatology</jtitle><addtitle>Eur J Gastroenterol Hepatol</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>8</volume><issue>8</issue><spage>805</spage><epage>809</epage><pages>805-809</pages><issn>0954-691X</issn><eissn>1473-5687</eissn><abstract>To document whether the administration of granulocyte colony-stimulating factor (G-CSF) enhances the impaired regenerative response of hepatocytes to partial hepatectomy (PH), in cadmium-pretreated partially hepatectomized rats.
Rats were injected intraperioneally with 2.5 mg CdCl2/kg body weight, 24h before PH. G-CSF (1500 or 150 micrograms/kg body weight) or saline was administered intraperitoneally in cadmium-pretreated partially hepatectomized rats at the same time as PH. The liver regenerative process was estimated 24h after PH. [3H] thymidine incorporation into liver DNA, liver thymidine kinase (TK) activity, mitotic index and proliferating cell nuclear antigen (PCNA) immunostaining were used as indices of hepatocyte proliferation.
G-CSF administration in cadmium-pretreated partially hepatectomized rats restored the suppressed DNA biosynthesis and TK activity (P < 0.001), to levels similar to those found in rats that were partially hepatectomized only. The mitotic index and the percentage of PCNA positive nuclei in hepatocytes were also enhanced in G-CSF administered cadmium-pretreated partially hepatectomized groups of rats.
The administration of G-CSF triggers events that restore the impaired liver regeneration in this model of reduced hepatocyte proliferation.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>8864679</pmid><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0954-691X |
| ispartof | European journal of gastroenterology & hepatology, 1996-08, Vol.8 (8), p.805-809 |
| issn | 0954-691X 1473-5687 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Biological and medical sciences Cadmium - toxicity Disease Models, Animal Granulocyte Colony-Stimulating Factor - pharmacology Hepatectomy Liver - cytology Liver - drug effects Liver - physiology Liver Regeneration - drug effects Liver, biliary tract, pancreas, portal circulation, spleen Male Medical sciences Mitotic Index Rats Rats, Wistar Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the digestive system |
| title | Granulocyte colony-stimulating factor administration reverses cadmium-associated inhibition of hepatocyte regeneration |
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