Vanadium(IV) causes 2'-deoxyguanosine hydroxylation and deoxyribonucleic acid damage via free radical reactions
Vanadium(IV) caused molecular oxygen dependent 2'-deoxyguanosine (dG) hydroxylation to form 8-hydroxyl-2'-deoxyguanosine (8-OHdG). During a 15-minute incubation of 1.0 mM dG and 1.0 mM VOSO4 (vanadium(IV)) in phosphate buffer solution (pH 7.4) at room temperature under ambient air, dG was...
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| Veröffentlicht in: | Annals of clinical and laboratory science 1996, Vol.26 (1), p.39-49 |
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| creator | Shi, X Wang, P Jiang, H Mao, Y Ahmed, N Dalal, N |
| description | Vanadium(IV) caused molecular oxygen dependent 2'-deoxyguanosine (dG) hydroxylation to form 8-hydroxyl-2'-deoxyguanosine (8-OHdG). During a 15-minute incubation of 1.0 mM dG and 1.0 mM VOSO4 (vanadium(IV)) in phosphate buffer solution (pH 7.4) at room temperature under ambient air, dG was converted to 8-OHdG with a yield of about 0.31 percent. Catalase and formate inhibited the 8-OHdG formation while superoxide dismutase enhanced it. Diethylenetriaminepentaacetic acid (DTPA) and deferoxamine blocked the 8-OHdG formation. Incubation of vanadium(IV) with dG in argon did not generate any significant amount of 8-OHdG, indicating the role of molecular oxygen in the mechanism of vanadium(IV)-induced dG hydroxylation. Vanadium(IV) also caused molecular oxygen dependent deoxyribonucleic acid (DNA) strand breaks in a pattern similar to that observed for dG hydroxylation. Reaction of vanadium(IV) with H2O2 generated OH radicals, which were inhibited by DTPA and deferoxamine. Incubation of vanadium(IV) with dG or with DNA in the presence of H2O2 resulted in an enhanced 8-OHdG formation and substantial DNA strand breaks. Reaction of vanadium(IV) with t-butyl hydroperoxide generated hydroperoxide-derived free radicals, which caused 8-OHdG formation from dG and DNA strand breaks. |
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During a 15-minute incubation of 1.0 mM dG and 1.0 mM VOSO4 (vanadium(IV)) in phosphate buffer solution (pH 7.4) at room temperature under ambient air, dG was converted to 8-OHdG with a yield of about 0.31 percent. Catalase and formate inhibited the 8-OHdG formation while superoxide dismutase enhanced it. Diethylenetriaminepentaacetic acid (DTPA) and deferoxamine blocked the 8-OHdG formation. Incubation of vanadium(IV) with dG in argon did not generate any significant amount of 8-OHdG, indicating the role of molecular oxygen in the mechanism of vanadium(IV)-induced dG hydroxylation. Vanadium(IV) also caused molecular oxygen dependent deoxyribonucleic acid (DNA) strand breaks in a pattern similar to that observed for dG hydroxylation. Reaction of vanadium(IV) with H2O2 generated OH radicals, which were inhibited by DTPA and deferoxamine. Incubation of vanadium(IV) with dG or with DNA in the presence of H2O2 resulted in an enhanced 8-OHdG formation and substantial DNA strand breaks. Reaction of vanadium(IV) with t-butyl hydroperoxide generated hydroperoxide-derived free radicals, which caused 8-OHdG formation from dG and DNA strand breaks.</description><identifier>ISSN: 0091-7370</identifier><identifier>EISSN: 1550-8080</identifier><identifier>PMID: 8834359</identifier><identifier>CODEN: ACLSCP</identifier><language>eng</language><publisher>Philadelphia, PA: Institute for Clinical Science</publisher><subject>Biological and medical sciences ; Chelating Agents - pharmacology ; Chemical and industrial products toxicology. Toxic occupational diseases ; Deoxyguanosine - metabolism ; DNA - drug effects ; DNA Damage ; General aspects ; Hydroxyl Radical - metabolism ; Hydroxylation ; Medical sciences ; Peroxides - metabolism ; tert-Butylhydroperoxide ; Toxicology ; Vanadium - toxicity</subject><ispartof>Annals of clinical and laboratory science, 1996, Vol.26 (1), p.39-49</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,4049,4050,23929,23930,25139</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2983326$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8834359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, X</creatorcontrib><creatorcontrib>Wang, P</creatorcontrib><creatorcontrib>Jiang, H</creatorcontrib><creatorcontrib>Mao, Y</creatorcontrib><creatorcontrib>Ahmed, N</creatorcontrib><creatorcontrib>Dalal, N</creatorcontrib><title>Vanadium(IV) causes 2'-deoxyguanosine hydroxylation and deoxyribonucleic acid damage via free radical reactions</title><title>Annals of clinical and laboratory science</title><addtitle>Ann Clin Lab Sci</addtitle><description>Vanadium(IV) caused molecular oxygen dependent 2'-deoxyguanosine (dG) hydroxylation to form 8-hydroxyl-2'-deoxyguanosine (8-OHdG). During a 15-minute incubation of 1.0 mM dG and 1.0 mM VOSO4 (vanadium(IV)) in phosphate buffer solution (pH 7.4) at room temperature under ambient air, dG was converted to 8-OHdG with a yield of about 0.31 percent. Catalase and formate inhibited the 8-OHdG formation while superoxide dismutase enhanced it. Diethylenetriaminepentaacetic acid (DTPA) and deferoxamine blocked the 8-OHdG formation. Incubation of vanadium(IV) with dG in argon did not generate any significant amount of 8-OHdG, indicating the role of molecular oxygen in the mechanism of vanadium(IV)-induced dG hydroxylation. Vanadium(IV) also caused molecular oxygen dependent deoxyribonucleic acid (DNA) strand breaks in a pattern similar to that observed for dG hydroxylation. Reaction of vanadium(IV) with H2O2 generated OH radicals, which were inhibited by DTPA and deferoxamine. Incubation of vanadium(IV) with dG or with DNA in the presence of H2O2 resulted in an enhanced 8-OHdG formation and substantial DNA strand breaks. Reaction of vanadium(IV) with t-butyl hydroperoxide generated hydroperoxide-derived free radicals, which caused 8-OHdG formation from dG and DNA strand breaks.</description><subject>Biological and medical sciences</subject><subject>Chelating Agents - pharmacology</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Deoxyguanosine - metabolism</subject><subject>DNA - drug effects</subject><subject>DNA Damage</subject><subject>General aspects</subject><subject>Hydroxyl Radical - metabolism</subject><subject>Hydroxylation</subject><subject>Medical sciences</subject><subject>Peroxides - metabolism</subject><subject>tert-Butylhydroperoxide</subject><subject>Toxicology</subject><subject>Vanadium - toxicity</subject><issn>0091-7370</issn><issn>1550-8080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9T0tLAzEYDKLUWv0JQg6CeljI5p2jFKuFghfttXybR43sZkvSFfvvXbV4GpgXMydoWgtBKk00OUVTQkxdKabIOboo5YMQajgnEzTRmnEmzBT1a0jg4tDdLdf32MJQfMH0tnK-_zpsB0h9icnj94PLI9HCPvYJQ3L415Bj06fBtj5aDDaOLHSw9fgzAg7Ze5zHbgstzh7sT7RcorMAbfFXR5yht8Xj6_y5Wr08LecPq2pHmdhXtRKNoSB1IM4Iy4gTVvi6USFwKUXjRc0FC5pzJziD4CTz1NUKlJFE1orN0PVf725oOu82uxw7yIfN8fio3xx1KOPAkCHZWP5t1GjGqGTfpoBjSA</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Shi, X</creator><creator>Wang, P</creator><creator>Jiang, H</creator><creator>Mao, Y</creator><creator>Ahmed, N</creator><creator>Dalal, N</creator><general>Institute for Clinical Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>1996</creationdate><title>Vanadium(IV) causes 2'-deoxyguanosine hydroxylation and deoxyribonucleic acid damage via free radical reactions</title><author>Shi, X ; Wang, P ; Jiang, H ; Mao, Y ; Ahmed, N ; Dalal, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-175b92a68f0d95c30d5c5e1b7ff4665be51453f844d543afd63e2d17a79606173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>Chelating Agents - pharmacology</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Deoxyguanosine - metabolism</topic><topic>DNA - drug effects</topic><topic>DNA Damage</topic><topic>General aspects</topic><topic>Hydroxyl Radical - metabolism</topic><topic>Hydroxylation</topic><topic>Medical sciences</topic><topic>Peroxides - metabolism</topic><topic>tert-Butylhydroperoxide</topic><topic>Toxicology</topic><topic>Vanadium - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, X</creatorcontrib><creatorcontrib>Wang, P</creatorcontrib><creatorcontrib>Jiang, H</creatorcontrib><creatorcontrib>Mao, Y</creatorcontrib><creatorcontrib>Ahmed, N</creatorcontrib><creatorcontrib>Dalal, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Annals of clinical and laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, X</au><au>Wang, P</au><au>Jiang, H</au><au>Mao, Y</au><au>Ahmed, N</au><au>Dalal, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vanadium(IV) causes 2'-deoxyguanosine hydroxylation and deoxyribonucleic acid damage via free radical reactions</atitle><jtitle>Annals of clinical and laboratory science</jtitle><addtitle>Ann Clin Lab Sci</addtitle><date>1996</date><risdate>1996</risdate><volume>26</volume><issue>1</issue><spage>39</spage><epage>49</epage><pages>39-49</pages><issn>0091-7370</issn><eissn>1550-8080</eissn><coden>ACLSCP</coden><abstract>Vanadium(IV) caused molecular oxygen dependent 2'-deoxyguanosine (dG) hydroxylation to form 8-hydroxyl-2'-deoxyguanosine (8-OHdG). During a 15-minute incubation of 1.0 mM dG and 1.0 mM VOSO4 (vanadium(IV)) in phosphate buffer solution (pH 7.4) at room temperature under ambient air, dG was converted to 8-OHdG with a yield of about 0.31 percent. Catalase and formate inhibited the 8-OHdG formation while superoxide dismutase enhanced it. Diethylenetriaminepentaacetic acid (DTPA) and deferoxamine blocked the 8-OHdG formation. Incubation of vanadium(IV) with dG in argon did not generate any significant amount of 8-OHdG, indicating the role of molecular oxygen in the mechanism of vanadium(IV)-induced dG hydroxylation. Vanadium(IV) also caused molecular oxygen dependent deoxyribonucleic acid (DNA) strand breaks in a pattern similar to that observed for dG hydroxylation. Reaction of vanadium(IV) with H2O2 generated OH radicals, which were inhibited by DTPA and deferoxamine. Incubation of vanadium(IV) with dG or with DNA in the presence of H2O2 resulted in an enhanced 8-OHdG formation and substantial DNA strand breaks. Reaction of vanadium(IV) with t-butyl hydroperoxide generated hydroperoxide-derived free radicals, which caused 8-OHdG formation from dG and DNA strand breaks.</abstract><cop>Philadelphia, PA</cop><pub>Institute for Clinical Science</pub><pmid>8834359</pmid><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0091-7370 |
| ispartof | Annals of clinical and laboratory science, 1996, Vol.26 (1), p.39-49 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Chelating Agents - pharmacology Chemical and industrial products toxicology. Toxic occupational diseases Deoxyguanosine - metabolism DNA - drug effects DNA Damage General aspects Hydroxyl Radical - metabolism Hydroxylation Medical sciences Peroxides - metabolism tert-Butylhydroperoxide Toxicology Vanadium - toxicity |
| title | Vanadium(IV) causes 2'-deoxyguanosine hydroxylation and deoxyribonucleic acid damage via free radical reactions |
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