Glycine at the NMDA receptor in cat visual cortex: saturation and changes with age
D. Czepita, N. W. Daw and S. N. Reid Department of Ophthalmology and Visual Science, Yale University Medical School, New Haven, Connecticut 06520-8061, USA. 1. Saturation of the glycine site at the N-methyl-D-aspartate (NMDA) receptor in cat visual cortex was tested by iontophoresing D-serine, and t...
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| Veröffentlicht in: | Journal of neurophysiology 1996-01, Vol.75 (1), p.311-317 |
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| creator | Czepita, D Daw, N. W Reid, S. N |
| description | D. Czepita, N. W. Daw and S. N. Reid
Department of Ophthalmology and Visual Science, Yale University Medical School, New Haven, Connecticut 06520-8061, USA.
1. Saturation of the glycine site at the N-methyl-D-aspartate (NMDA)
receptor in cat visual cortex was tested by iontophoresing D-serine, and
the contribution of this site to the visual response was tested by
iontophoresing 7-chlorokynurenic acid (7-Cl-KYNA). Animals were tested at
ages 3 wk, 6 wk, 5 mo, and adult. 2. In at least 40% of cells (24/57),
D-serine increased the response significantly, showing that the glycine
site was not saturated. However, the increase was rarely > 100%. 3. The
amount by which D-serine increased the response did not vary significantly
with age. 4. In five cases there was positive evidence for saturation of
the glycine site. D-Serine did not increase the response significantly;
however, when D-serine was applied on top of 7-Cl-KYNA, it did increase the
response compared with that seen with 7-Cl-KYNA alone. These cases were all
in 3-wk-old animals. In other cases (28/57), D-serine did not increase the
response significantly, but we could not be absolutely certain that the
D-serine had reached the site of action. 5. The effect of 7-Cl-KYNA was
largest in 3-wk animals, in agreement with previous findings that the
effect of D-2-amino-5-phosphonovalerate (APV) is largest at this age. 6.
These results may provide a small part of the explanation for the finding
that the NMDA receptor-mediated component of the visual response decreases
between 3 and 6 wk of age, while the number of NMDA receptors is
increasing. However, the magnitude of the results make it likely that other
factors are more important. |
| doi_str_mv | 10.1152/jn.1996.75.1.311 |
| format | Article |
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Department of Ophthalmology and Visual Science, Yale University Medical School, New Haven, Connecticut 06520-8061, USA.
1. Saturation of the glycine site at the N-methyl-D-aspartate (NMDA)
receptor in cat visual cortex was tested by iontophoresing D-serine, and
the contribution of this site to the visual response was tested by
iontophoresing 7-chlorokynurenic acid (7-Cl-KYNA). Animals were tested at
ages 3 wk, 6 wk, 5 mo, and adult. 2. In at least 40% of cells (24/57),
D-serine increased the response significantly, showing that the glycine
site was not saturated. However, the increase was rarely > 100%. 3. The
amount by which D-serine increased the response did not vary significantly
with age. 4. In five cases there was positive evidence for saturation of
the glycine site. D-Serine did not increase the response significantly;
however, when D-serine was applied on top of 7-Cl-KYNA, it did increase the
response compared with that seen with 7-Cl-KYNA alone. These cases were all
in 3-wk-old animals. In other cases (28/57), D-serine did not increase the
response significantly, but we could not be absolutely certain that the
D-serine had reached the site of action. 5. The effect of 7-Cl-KYNA was
largest in 3-wk animals, in agreement with previous findings that the
effect of D-2-amino-5-phosphonovalerate (APV) is largest at this age. 6.
These results may provide a small part of the explanation for the finding
that the NMDA receptor-mediated component of the visual response decreases
between 3 and 6 wk of age, while the number of NMDA receptors is
increasing. However, the magnitude of the results make it likely that other
factors are more important.</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.1996.75.1.311</identifier><identifier>PMID: 8822559</identifier><language>eng</language><publisher>United States: Am Phys Soc</publisher><subject>Aging - physiology ; Animals ; Animals, Newborn ; Cats ; Evoked Potentials, Visual - physiology ; glycine ; Glycine - physiology ; Iontophoresis ; Neurons - physiology ; Receptors, N-Methyl-D-Aspartate - physiology ; Synaptic Transmission - physiology ; Visual Cortex - physiology</subject><ispartof>Journal of neurophysiology, 1996-01, Vol.75 (1), p.311-317</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-67bde82500b0d28dad7d86cf397e8879f657c5f90f71641f050682ee9f37cd233</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8822559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Czepita, D</creatorcontrib><creatorcontrib>Daw, N. W</creatorcontrib><creatorcontrib>Reid, S. N</creatorcontrib><title>Glycine at the NMDA receptor in cat visual cortex: saturation and changes with age</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description>D. Czepita, N. W. Daw and S. N. Reid
Department of Ophthalmology and Visual Science, Yale University Medical School, New Haven, Connecticut 06520-8061, USA.
1. Saturation of the glycine site at the N-methyl-D-aspartate (NMDA)
receptor in cat visual cortex was tested by iontophoresing D-serine, and
the contribution of this site to the visual response was tested by
iontophoresing 7-chlorokynurenic acid (7-Cl-KYNA). Animals were tested at
ages 3 wk, 6 wk, 5 mo, and adult. 2. In at least 40% of cells (24/57),
D-serine increased the response significantly, showing that the glycine
site was not saturated. However, the increase was rarely > 100%. 3. The
amount by which D-serine increased the response did not vary significantly
with age. 4. In five cases there was positive evidence for saturation of
the glycine site. D-Serine did not increase the response significantly;
however, when D-serine was applied on top of 7-Cl-KYNA, it did increase the
response compared with that seen with 7-Cl-KYNA alone. These cases were all
in 3-wk-old animals. In other cases (28/57), D-serine did not increase the
response significantly, but we could not be absolutely certain that the
D-serine had reached the site of action. 5. The effect of 7-Cl-KYNA was
largest in 3-wk animals, in agreement with previous findings that the
effect of D-2-amino-5-phosphonovalerate (APV) is largest at this age. 6.
These results may provide a small part of the explanation for the finding
that the NMDA receptor-mediated component of the visual response decreases
between 3 and 6 wk of age, while the number of NMDA receptors is
increasing. However, the magnitude of the results make it likely that other
factors are more important.</description><subject>Aging - physiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Cats</subject><subject>Evoked Potentials, Visual - physiology</subject><subject>glycine</subject><subject>Glycine - physiology</subject><subject>Iontophoresis</subject><subject>Neurons - physiology</subject><subject>Receptors, N-Methyl-D-Aspartate - physiology</subject><subject>Synaptic Transmission - physiology</subject><subject>Visual Cortex - physiology</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv2zAMRoVhRZt2u-9SQKfd4lJSZUm7Be2aFkg7YNjOgiLTsQLHziR5Wf59HSTojj2RID8-Ao-QLwwKxiS_WXcFM6YslCxYIRj7QCbjmE-ZNPojmQCMvQClLshlSmsAUBL4OTnXmnMpzYT8nLd7HzqkLtPcIH15vp_RiB63uY80dNSPi78hDa6lvo8Z_32jyeUhuhz6jrquor5x3QoT3YXcULfCT-Ssdm3Cz6d6RX4_fP919zhd_Jg_3c0WUy9kmaelWlaouQRYQsV15SpV6dLXwijUWpm6lMrL2kCtWHnLapBQao5oaqF8xYW4Il-P3G3s_wyYst2E5LFtXYf9kKzSQoG4fT_IFEhhzCEIx6CPfUoRa7uNYePi3jKwB9923dmDb6ukZXb0PZ5cn9jDcoPV28FJ8P_fTVg1uxDRbpt9Cn3br_YH2hvoFay7iAU</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>Czepita, D</creator><creator>Daw, N. W</creator><creator>Reid, S. N</creator><general>Am Phys Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19960101</creationdate><title>Glycine at the NMDA receptor in cat visual cortex: saturation and changes with age</title><author>Czepita, D ; Daw, N. W ; Reid, S. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-67bde82500b0d28dad7d86cf397e8879f657c5f90f71641f050682ee9f37cd233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Aging - physiology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Cats</topic><topic>Evoked Potentials, Visual - physiology</topic><topic>glycine</topic><topic>Glycine - physiology</topic><topic>Iontophoresis</topic><topic>Neurons - physiology</topic><topic>Receptors, N-Methyl-D-Aspartate - physiology</topic><topic>Synaptic Transmission - physiology</topic><topic>Visual Cortex - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Czepita, D</creatorcontrib><creatorcontrib>Daw, N. W</creatorcontrib><creatorcontrib>Reid, S. N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Czepita, D</au><au>Daw, N. W</au><au>Reid, S. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycine at the NMDA receptor in cat visual cortex: saturation and changes with age</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>1996-01-01</date><risdate>1996</risdate><volume>75</volume><issue>1</issue><spage>311</spage><epage>317</epage><pages>311-317</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract>D. Czepita, N. W. Daw and S. N. Reid
Department of Ophthalmology and Visual Science, Yale University Medical School, New Haven, Connecticut 06520-8061, USA.
1. Saturation of the glycine site at the N-methyl-D-aspartate (NMDA)
receptor in cat visual cortex was tested by iontophoresing D-serine, and
the contribution of this site to the visual response was tested by
iontophoresing 7-chlorokynurenic acid (7-Cl-KYNA). Animals were tested at
ages 3 wk, 6 wk, 5 mo, and adult. 2. In at least 40% of cells (24/57),
D-serine increased the response significantly, showing that the glycine
site was not saturated. However, the increase was rarely > 100%. 3. The
amount by which D-serine increased the response did not vary significantly
with age. 4. In five cases there was positive evidence for saturation of
the glycine site. D-Serine did not increase the response significantly;
however, when D-serine was applied on top of 7-Cl-KYNA, it did increase the
response compared with that seen with 7-Cl-KYNA alone. These cases were all
in 3-wk-old animals. In other cases (28/57), D-serine did not increase the
response significantly, but we could not be absolutely certain that the
D-serine had reached the site of action. 5. The effect of 7-Cl-KYNA was
largest in 3-wk animals, in agreement with previous findings that the
effect of D-2-amino-5-phosphonovalerate (APV) is largest at this age. 6.
These results may provide a small part of the explanation for the finding
that the NMDA receptor-mediated component of the visual response decreases
between 3 and 6 wk of age, while the number of NMDA receptors is
increasing. However, the magnitude of the results make it likely that other
factors are more important.</abstract><cop>United States</cop><pub>Am Phys Soc</pub><pmid>8822559</pmid><doi>10.1152/jn.1996.75.1.311</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aging - physiology Animals Animals, Newborn Cats Evoked Potentials, Visual - physiology glycine Glycine - physiology Iontophoresis Neurons - physiology Receptors, N-Methyl-D-Aspartate - physiology Synaptic Transmission - physiology Visual Cortex - physiology |
| title | Glycine at the NMDA receptor in cat visual cortex: saturation and changes with age |
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