Production of a paraquat-specific murine single chain Fv fragment

Producing an effective antidote against poisoning by the herbicide paraquat (PQ) has proven to be an elusive goal. One approach that holds some promise is immunotherapy with antibody fragments. In this study we detail the production of a single chain Fv fragment (scFv) specific for paraquat by linki...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1995-09, Vol.118 (3), p.480-487
Hauptverfasser:	Devlin, C.M, Bowles, M.R, Gordon, R.B, Pond, S.M
Format:	Artikel
Sprache:	eng
Schlagworte:	1‘-dimethyl-4 4’-bipyridinium dichloride Amino Acid Sequence amino acid sequences Animals antibodies Antibody Specificity Base Sequence Binding Sites, Antibody cloning Cloning, Molecular DNA, Complementary - genetics Gene Amplification Gene Expression genes Genes, Immunoglobulin Herbicides - immunology Herbicides - metabolism Herbicides - toxicity Immunoglobulin Fragments - biosynthesis Immunoglobulin Fragments - genetics Immunoglobulin Fragments - pharmacology Immunoglobulin Variable Region - biosynthesis Immunoglobulin Variable Region - genetics Kinetics Mice Molecular Sequence Data murine antibody nucleotide sequences paraquat Paraquat - immunology Paraquat - metabolism Paraquat - toxicity Polymerase Chain Reaction protein refolding Rabbits single chain antibody surface plasmon resonance
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