Cardioprotective Effects of 70-kDa Heat Shock Protein in Transgenic Mice
Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat sh...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-03, Vol.93 (6), p.2339-2342 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 93 |
| creator | Radford, Nina B. Fina, Maggy Benjamin, Ivor J. Moreadith, Randall W. Graves, Kathy H. Zhao, Piyu Gavva, Sandhya Wiethoff, Andrea Sherry, A. Dean Malloy, Craig R. Williams, R. Sanders |
| description | Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart. |
| doi_str_mv | 10.1073/pnas.93.6.2339 |
| format | Article |
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These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.93.6.2339</identifier><identifier>PMID: 8637874</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adenine Nucleotides - metabolism ; Animals ; Cardiovascular disease ; Complementary DNA ; Cultured cells ; Energy Metabolism ; Heart ; Heat shock proteins ; HSP70 Heat-Shock Proteins - physiology ; Humans ; Ischemia ; Magnetic Resonance Spectroscopy ; Metabolism ; Mice ; Mice, Transgenic ; Myocardium - metabolism ; Phosphates ; Proteins ; Reperfusion Injury - metabolism ; Rodents ; Transgenes ; Transgenic animals</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-03, Vol.93 (6), p.2339-2342</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Mar 19, 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-8c729e8ff8b5278eefe0a1a27a828b2279c3f1b0466874653a53a104c976d8623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/93/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/38674$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/38674$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8637874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radford, Nina B.</creatorcontrib><creatorcontrib>Fina, Maggy</creatorcontrib><creatorcontrib>Benjamin, Ivor J.</creatorcontrib><creatorcontrib>Moreadith, Randall W.</creatorcontrib><creatorcontrib>Graves, Kathy H.</creatorcontrib><creatorcontrib>Zhao, Piyu</creatorcontrib><creatorcontrib>Gavva, Sandhya</creatorcontrib><creatorcontrib>Wiethoff, Andrea</creatorcontrib><creatorcontrib>Sherry, A. Dean</creatorcontrib><creatorcontrib>Malloy, Craig R.</creatorcontrib><creatorcontrib>Williams, R. Sanders</creatorcontrib><title>Cardioprotective Effects of 70-kDa Heat Shock Protein in Transgenic Mice</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.</description><subject>Adenine Nucleotides - metabolism</subject><subject>Animals</subject><subject>Cardiovascular disease</subject><subject>Complementary DNA</subject><subject>Cultured cells</subject><subject>Energy Metabolism</subject><subject>Heart</subject><subject>Heat shock proteins</subject><subject>HSP70 Heat-Shock Proteins - physiology</subject><subject>Humans</subject><subject>Ischemia</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Myocardium - metabolism</subject><subject>Phosphates</subject><subject>Proteins</subject><subject>Reperfusion Injury - metabolism</subject><subject>Rodents</subject><subject>Transgenes</subject><subject>Transgenic animals</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUx4Moda1ePQjC4KG3mb4kM_kBXmStbqFFwXoO2WzSZjs7WZNM0f_eDLtdVg8KDxL4fr7vveSL0GsMDQZOz7eDTo2kDWsIpfIJmmGQuGathKdoBkB4LVrSPkcvUloDgOwEnKATwSgXvJ2hxVzHlQ_bGLI12T_Y6sK5cktVcBWH-v6jrhZW5-rbXTD31deJ80NV6ibqId3awZvq2hv7Ej1zuk_21f48Rd8_XdzMF_XVl8-X8w9Xtem4zLUwnEgrnBPLjnBhrbOgsSZcCyKWhHBpqMNLaBkr-7GO6lIYWiM5WwlG6Cl6v-u7HZcbuzJ2yFH3ahv9RsdfKmiv_lQGf6duw4Oikkte7Gd7eww_Rpuy2vhkbN_rwYYxKc6l7Dqg_wUxh45i1hbw3V_gOoxxKH-gCGDa0tKxQM0OMjGkFK07LIxBTTmqKUclqWJqyrEY3h4_84DvgzvSJ9-jeuw_-5eu3Nj32f7MBXyzA9cph3ggqWBlym85drhx</recordid><startdate>19960319</startdate><enddate>19960319</enddate><creator>Radford, Nina B.</creator><creator>Fina, Maggy</creator><creator>Benjamin, Ivor J.</creator><creator>Moreadith, Randall W.</creator><creator>Graves, Kathy H.</creator><creator>Zhao, Piyu</creator><creator>Gavva, Sandhya</creator><creator>Wiethoff, Andrea</creator><creator>Sherry, A. 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| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-03, Vol.93 (6), p.2339-2342 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_8637874 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenine Nucleotides - metabolism Animals Cardiovascular disease Complementary DNA Cultured cells Energy Metabolism Heart Heat shock proteins HSP70 Heat-Shock Proteins - physiology Humans Ischemia Magnetic Resonance Spectroscopy Metabolism Mice Mice, Transgenic Myocardium - metabolism Phosphates Proteins Reperfusion Injury - metabolism Rodents Transgenes Transgenic animals |
| title | Cardioprotective Effects of 70-kDa Heat Shock Protein in Transgenic Mice |
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