Expression of inducible nitric oxide synthase in the eye from endotoxin- induced uveitis rats
Inducible nitric oxide (NO) synthase (iNOS) has been implicated in the pathogenesis of endotoxin-induced uveitis (EIU). This study was undertaken to localize the cells, in the eye, which express iNOS during EIU in the rat. EIU was induced in Lewis rats by a single foot pad injection of 150 microgram...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1996-05, Vol.37 (6), p.1187-1196 |
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| creator | Jacquemin, E de Kozak, Y Thillaye, B Courtois, Y Goureau, O |
| description | Inducible nitric oxide (NO) synthase (iNOS) has been implicated in the pathogenesis of endotoxin-induced uveitis (EIU). This study was undertaken to localize the cells, in the eye, which express iNOS during EIU in the rat.
EIU was induced in Lewis rats by a single foot pad injection of 150 micrograms lipopolysaccharide (LPS) from Salmonella typhimurium. At different time intervals after LPS injection, the authors evaluated ocular inflammation (slit lamp observation), iNOS localization by in situ hybridization, and comparison of OX-42- and ED1-positive cell appearance and of glial response by specific immunohistochemistry.
iNOS mRNA was not detected in the iris-ciliary body nor in the retina of control rats. It was detected strongly in the epithelial cells of the iris-ciliary body at 6 hours and also in stromal cells of the ciliary processes at 16 hours after LPS injection. In the neuroretina, iNOS mRNA was observed in the inner layers 16 hours after LPS injection. iNOS-positive cells were also present on the vitreous at this time. At 6 and approximately 16 hours after LPS injection, immunohistochemistry experiments revealed a large number of OX-42- and ED1-positive cells (microglia, macrophages, or polymorphonuclear leukocytes) colocalized in part with some iNOS-positive cells in the ciliary body and in the retina. Furthermore, expression of iNOS in Müller cells cannot be excluded.
These observations confirm that subcutaneous injection of endotoxin dramatically induces NOS mRNA expression in the eye, and they demonstrate that epithelial cells of the iris-ciliary body and cells infiltrating the anterior segment of the eye and the retina are the major source of NO. These results support the hypothesis that both inflammatory and resident ocular cells are involved in iNOS expression during EIU. |
| format | Article |
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EIU was induced in Lewis rats by a single foot pad injection of 150 micrograms lipopolysaccharide (LPS) from Salmonella typhimurium. At different time intervals after LPS injection, the authors evaluated ocular inflammation (slit lamp observation), iNOS localization by in situ hybridization, and comparison of OX-42- and ED1-positive cell appearance and of glial response by specific immunohistochemistry.
iNOS mRNA was not detected in the iris-ciliary body nor in the retina of control rats. It was detected strongly in the epithelial cells of the iris-ciliary body at 6 hours and also in stromal cells of the ciliary processes at 16 hours after LPS injection. In the neuroretina, iNOS mRNA was observed in the inner layers 16 hours after LPS injection. iNOS-positive cells were also present on the vitreous at this time. At 6 and approximately 16 hours after LPS injection, immunohistochemistry experiments revealed a large number of OX-42- and ED1-positive cells (microglia, macrophages, or polymorphonuclear leukocytes) colocalized in part with some iNOS-positive cells in the ciliary body and in the retina. Furthermore, expression of iNOS in Müller cells cannot be excluded.
These observations confirm that subcutaneous injection of endotoxin dramatically induces NOS mRNA expression in the eye, and they demonstrate that epithelial cells of the iris-ciliary body and cells infiltrating the anterior segment of the eye and the retina are the major source of NO. These results support the hypothesis that both inflammatory and resident ocular cells are involved in iNOS expression during EIU.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 8631633</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Animals ; Anterior Eye Segment - enzymology ; Anterior Eye Segment - pathology ; Base Sequence ; Biological and medical sciences ; Ciliary Body - enzymology ; Ciliary Body - pathology ; DNA Primers - chemistry ; DNA Probes ; DNA, Single-Stranded ; Enzyme Induction ; Eye - enzymology ; Eye - pathology ; Fluorescent Antibody Technique ; Gene Expression Regulation, Enzymologic ; In Situ Hybridization ; Iris - enzymology ; Iris - pathology ; Lipopolysaccharides ; Male ; Medical sciences ; Molecular Sequence Data ; Nitric Oxide Synthase - analysis ; Nitric Oxide Synthase - biosynthesis ; Ophthalmology ; Pigment Epithelium of Eye - enzymology ; Pigment Epithelium of Eye - pathology ; Rats ; Rats, Inbred BN ; Rats, Inbred Lew ; Retina - enzymology ; Retina - pathology ; RNA, Messenger - biosynthesis ; Salmonella typhimurium ; Uvea diseases ; Uveitis - chemically induced ; Uveitis - enzymology ; Uveitis - pathology</subject><ispartof>Investigative ophthalmology & visual science, 1996-05, Vol.37 (6), p.1187-1196</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3087298$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8631633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jacquemin, E</creatorcontrib><creatorcontrib>de Kozak, Y</creatorcontrib><creatorcontrib>Thillaye, B</creatorcontrib><creatorcontrib>Courtois, Y</creatorcontrib><creatorcontrib>Goureau, O</creatorcontrib><title>Expression of inducible nitric oxide synthase in the eye from endotoxin- induced uveitis rats</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>Inducible nitric oxide (NO) synthase (iNOS) has been implicated in the pathogenesis of endotoxin-induced uveitis (EIU). This study was undertaken to localize the cells, in the eye, which express iNOS during EIU in the rat.
EIU was induced in Lewis rats by a single foot pad injection of 150 micrograms lipopolysaccharide (LPS) from Salmonella typhimurium. At different time intervals after LPS injection, the authors evaluated ocular inflammation (slit lamp observation), iNOS localization by in situ hybridization, and comparison of OX-42- and ED1-positive cell appearance and of glial response by specific immunohistochemistry.
iNOS mRNA was not detected in the iris-ciliary body nor in the retina of control rats. It was detected strongly in the epithelial cells of the iris-ciliary body at 6 hours and also in stromal cells of the ciliary processes at 16 hours after LPS injection. In the neuroretina, iNOS mRNA was observed in the inner layers 16 hours after LPS injection. iNOS-positive cells were also present on the vitreous at this time. At 6 and approximately 16 hours after LPS injection, immunohistochemistry experiments revealed a large number of OX-42- and ED1-positive cells (microglia, macrophages, or polymorphonuclear leukocytes) colocalized in part with some iNOS-positive cells in the ciliary body and in the retina. Furthermore, expression of iNOS in Müller cells cannot be excluded.
These observations confirm that subcutaneous injection of endotoxin dramatically induces NOS mRNA expression in the eye, and they demonstrate that epithelial cells of the iris-ciliary body and cells infiltrating the anterior segment of the eye and the retina are the major source of NO. These results support the hypothesis that both inflammatory and resident ocular cells are involved in iNOS expression during EIU.</description><subject>Animals</subject><subject>Anterior Eye Segment - enzymology</subject><subject>Anterior Eye Segment - pathology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Ciliary Body - enzymology</subject><subject>Ciliary Body - pathology</subject><subject>DNA Primers - chemistry</subject><subject>DNA Probes</subject><subject>DNA, Single-Stranded</subject><subject>Enzyme Induction</subject><subject>Eye - enzymology</subject><subject>Eye - pathology</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>In Situ Hybridization</subject><subject>Iris - enzymology</subject><subject>Iris - pathology</subject><subject>Lipopolysaccharides</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nitric Oxide Synthase - analysis</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Ophthalmology</subject><subject>Pigment Epithelium of Eye - enzymology</subject><subject>Pigment Epithelium of Eye - pathology</subject><subject>Rats</subject><subject>Rats, Inbred BN</subject><subject>Rats, Inbred Lew</subject><subject>Retina - enzymology</subject><subject>Retina - pathology</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Salmonella typhimurium</subject><subject>Uvea diseases</subject><subject>Uveitis - chemically induced</subject><subject>Uveitis - enzymology</subject><subject>Uveitis - pathology</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j0tLxDAUhYMo4zj6E4QsxF0hrybpUobxAQNudCkl09zYSJsOSWtn_r2BGbybu_i-c-BcoCUtS1aUSvNLtCRUyIIIIq7RTUo_hDBKGVmghZacSs6X6Gtz2EdIyQ8BDw77YKfG7zrAwY_RN3g4eAs4HcPYmgSZ47EFDEfALg49hmCHMTuhOEXB4ukX_OgTjmZMt-jKmS7B3fmv0Ofz5mP9WmzfX97WT9uiZVKOhRb5nNgRLhiThJdW0FIbIIY5ZXQlRMlVnsG4lMxVthEEKuMcVVaCKhVfoftT737a9WDrffS9icf6PDPzhzM3qTGdiyY0Pv1rnGjFKp21x5PW-u929hHq1Juuy6W0nueZq1rWlGrF_wBgt2kk</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Jacquemin, E</creator><creator>de Kozak, Y</creator><creator>Thillaye, B</creator><creator>Courtois, Y</creator><creator>Goureau, O</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19960501</creationdate><title>Expression of inducible nitric oxide synthase in the eye from endotoxin- induced uveitis rats</title><author>Jacquemin, E ; de Kozak, Y ; Thillaye, B ; Courtois, Y ; Goureau, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-84444f4b034226035d4158ae0a2f7a894453704023662f9dc40e9aff17d6e7573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Anterior Eye Segment - enzymology</topic><topic>Anterior Eye Segment - pathology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Ciliary Body - enzymology</topic><topic>Ciliary Body - pathology</topic><topic>DNA Primers - chemistry</topic><topic>DNA Probes</topic><topic>DNA, Single-Stranded</topic><topic>Enzyme Induction</topic><topic>Eye - enzymology</topic><topic>Eye - pathology</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>In Situ Hybridization</topic><topic>Iris - enzymology</topic><topic>Iris - pathology</topic><topic>Lipopolysaccharides</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nitric Oxide Synthase - analysis</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Ophthalmology</topic><topic>Pigment Epithelium of Eye - enzymology</topic><topic>Pigment Epithelium of Eye - pathology</topic><topic>Rats</topic><topic>Rats, Inbred BN</topic><topic>Rats, Inbred Lew</topic><topic>Retina - enzymology</topic><topic>Retina - pathology</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Salmonella typhimurium</topic><topic>Uvea diseases</topic><topic>Uveitis - chemically induced</topic><topic>Uveitis - enzymology</topic><topic>Uveitis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jacquemin, E</creatorcontrib><creatorcontrib>de Kozak, Y</creatorcontrib><creatorcontrib>Thillaye, B</creatorcontrib><creatorcontrib>Courtois, Y</creatorcontrib><creatorcontrib>Goureau, O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jacquemin, E</au><au>de Kozak, Y</au><au>Thillaye, B</au><au>Courtois, Y</au><au>Goureau, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of inducible nitric oxide synthase in the eye from endotoxin- induced uveitis rats</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>37</volume><issue>6</issue><spage>1187</spage><epage>1196</epage><pages>1187-1196</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>Inducible nitric oxide (NO) synthase (iNOS) has been implicated in the pathogenesis of endotoxin-induced uveitis (EIU). This study was undertaken to localize the cells, in the eye, which express iNOS during EIU in the rat.
EIU was induced in Lewis rats by a single foot pad injection of 150 micrograms lipopolysaccharide (LPS) from Salmonella typhimurium. At different time intervals after LPS injection, the authors evaluated ocular inflammation (slit lamp observation), iNOS localization by in situ hybridization, and comparison of OX-42- and ED1-positive cell appearance and of glial response by specific immunohistochemistry.
iNOS mRNA was not detected in the iris-ciliary body nor in the retina of control rats. It was detected strongly in the epithelial cells of the iris-ciliary body at 6 hours and also in stromal cells of the ciliary processes at 16 hours after LPS injection. In the neuroretina, iNOS mRNA was observed in the inner layers 16 hours after LPS injection. iNOS-positive cells were also present on the vitreous at this time. At 6 and approximately 16 hours after LPS injection, immunohistochemistry experiments revealed a large number of OX-42- and ED1-positive cells (microglia, macrophages, or polymorphonuclear leukocytes) colocalized in part with some iNOS-positive cells in the ciliary body and in the retina. Furthermore, expression of iNOS in Müller cells cannot be excluded.
These observations confirm that subcutaneous injection of endotoxin dramatically induces NOS mRNA expression in the eye, and they demonstrate that epithelial cells of the iris-ciliary body and cells infiltrating the anterior segment of the eye and the retina are the major source of NO. These results support the hypothesis that both inflammatory and resident ocular cells are involved in iNOS expression during EIU.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>8631633</pmid><tpages>10</tpages></addata></record> |
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| ispartof | Investigative ophthalmology & visual science, 1996-05, Vol.37 (6), p.1187-1196 |
| issn | 0146-0404 1552-5783 |
| language | eng |
| recordid | cdi_pubmed_primary_8631633 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Anterior Eye Segment - enzymology Anterior Eye Segment - pathology Base Sequence Biological and medical sciences Ciliary Body - enzymology Ciliary Body - pathology DNA Primers - chemistry DNA Probes DNA, Single-Stranded Enzyme Induction Eye - enzymology Eye - pathology Fluorescent Antibody Technique Gene Expression Regulation, Enzymologic In Situ Hybridization Iris - enzymology Iris - pathology Lipopolysaccharides Male Medical sciences Molecular Sequence Data Nitric Oxide Synthase - analysis Nitric Oxide Synthase - biosynthesis Ophthalmology Pigment Epithelium of Eye - enzymology Pigment Epithelium of Eye - pathology Rats Rats, Inbred BN Rats, Inbred Lew Retina - enzymology Retina - pathology RNA, Messenger - biosynthesis Salmonella typhimurium Uvea diseases Uveitis - chemically induced Uveitis - enzymology Uveitis - pathology |
| title | Expression of inducible nitric oxide synthase in the eye from endotoxin- induced uveitis rats |
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