Isolation of a species-specific mitochondrial DNA sequence for identification of Tilletia indica, the karnal bunt of wheat fungus

Mitochondrial DNA (mtDNA) from five isolates of Tilletia indica was isolated and digested with several restriction enzymes. A 2.3-kb EcoRI fragment was chosen, cloned, and shown to hybridize with total DNA restricted with EcoRI from T. indica and not from a morphologically similar smut fungus, Tille...

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Veröffentlicht in:	Applied and Environmental Microbiology 1996-01, Vol.62 (1), p.87-93
Hauptverfasser:	Ferreira, M.A.S.V. (Universidade de Brasilia, Brasilia, Brazil.), Tooley, P.W, Hatziloukas, E, Castro, C, Schaad, N.W
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN AMPLIFICATION CHAINE POLYMERASE Base Sequence Biological and medical sciences Cellular biology CHAMPIGNON Cloning, Molecular Deoxyribonucleic acid DNA DNA Primers DNA, Fungal - analysis DNA, Fungal - genetics DNA, Fungal - isolation & purification DNA, Mitochondrial - analysis DNA, Mitochondrial - genetics DNA, Mitochondrial - isolation & purification Enzymes ESPORAS Fundamental and applied biological sciences. Psychology Fungi HIBRIDACION DE ADN HONGOS HYBRIDATION D'ADN IDENTIFICACION IDENTIFICATION Microbiology MITOCHONDRIE MITOCONDRIA Molecular Sequence Data Mycological methods and techniques used in mycology Mycology ORGANISME TRANSMISSIBLE PAR SEMENCE ORGANISMOS TRANSMITIDOS POR SEMILLA Polymerase Chain Reaction - methods REACCION DE CADENAS DE POLIMERASA SECUENCIA NUCLEICA Sensitivity and Specificity Sequence Analysis, DNA SEQUENCE NUCLEIQUE Species Specificity SPORE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS TILLETIA Tilletia barclayana TILLETIA INDICA Triticum - microbiology TRITICUM AESTIVUM Ustilaginales - genetics Wheat
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