Mechanism of the Reaction Catalyzed by Acetoacetate Decarboxylase. Importance of Lysine 116 in Determining the pKa of Active-Site Lysine 115

Acetoacetate decarboxylase from Clostridium acetobutylicum (AAD) catalyzes the decarboxylation of acetoacetate via a Schiff base intermediate [Hamilton, G. A., & Westheimer, F. H. (1959) J. Am. Chem. Soc. 81, 6332; Fridovich, I., & Westheimer F. H. (1962) J. Am. Chem. Soc. 84, 3208]. The pKa...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemistry (Easton) 1996-01, Vol.35 (1), p.41-46
Hauptverfasser:	HIGHBARGER, Lane A., GERLT, John A., KENYON, George L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Binding Sites Carboxy-Lyases - chemistry Carboxy-Lyases - isolation & purification Carboxy-Lyases - metabolism Chromatography, High Pressure Liquid Chromatography, Ion Exchange Clostridium - enzymology DNA Primers Kinetics Lysine Molecular Sequence Data Mutagenesis, Site-Directed Peptide Fragments - chemistry Peptide Fragments - isolation & purification Point Mutation Polymerase Chain Reaction Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Schiff Bases Spectrophotometry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!