Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses
The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular c...
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| Veröffentlicht in: | Journal of Virology 1996-02, Vol.70 (2), p.1213-1217 |
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| container_title | Journal of Virology |
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| creator | Turelli, P. (INSERM, Marseille, France.) Petursson, G Guiguen, F Mornex, J.F Vigne, R Querat, G |
| description | The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase-defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages |
| doi_str_mv | 10.1128/jvi.70.2.1213-1217.1996 |
| format | Article |
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(INSERM, Marseille, France.) ; Petursson, G ; Guiguen, F ; Mornex, J.F ; Vigne, R ; Querat, G</creator><creatorcontrib>Turelli, P. (INSERM, Marseille, France.) ; Petursson, G ; Guiguen, F ; Mornex, J.F ; Vigne, R ; Querat, G</creatorcontrib><description>The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase-defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.70.2.1213-1217.1996</identifier><identifier>PMID: 8551582</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Animals ; Arthritis-Encephalitis Virus, Caprine - enzymology ; Arthritis-Encephalitis Virus, Caprine - physiology ; Base Sequence ; Cells, Cultured ; DNA, Viral ; HIDROLASAS ; Humans ; HYDROLASE ; MACROFAGOS ; MACROPHAGE ; Macrophages - cytology ; Molecular Sequence Data ; MUTANT ; MUTANTES ; Mutation ; Pyrophosphatases - genetics ; Pyrophosphatases - metabolism ; Sequence Homology, Amino Acid ; Thymine Nucleotides - metabolism ; VIRUS ARTHRITE ENCEPHALITE CAPRINE ; VIRUS ARTRITIS ENCEFALITIS CAPRINA ; Virus Replication - physiology ; VIRUS VISNA MAEDI ; visna virus ; Visna-maedi virus - enzymology ; Visna-maedi virus - physiology</subject><ispartof>Journal of Virology, 1996-02, Vol.70 (2), p.1213-1217</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-2e14c7643cb9b16c4860da946edf624cd076791c6f6188c6be5733e7d37a9c7b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC189930/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC189930/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8551582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turelli, P. (INSERM, Marseille, France.)</creatorcontrib><creatorcontrib>Petursson, G</creatorcontrib><creatorcontrib>Guiguen, F</creatorcontrib><creatorcontrib>Mornex, J.F</creatorcontrib><creatorcontrib>Vigne, R</creatorcontrib><creatorcontrib>Querat, G</creatorcontrib><title>Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase-defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Animals</subject><subject>Arthritis-Encephalitis Virus, Caprine - enzymology</subject><subject>Arthritis-Encephalitis Virus, Caprine - physiology</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>DNA, Viral</subject><subject>HIDROLASAS</subject><subject>Humans</subject><subject>HYDROLASE</subject><subject>MACROFAGOS</subject><subject>MACROPHAGE</subject><subject>Macrophages - cytology</subject><subject>Molecular Sequence Data</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Mutation</subject><subject>Pyrophosphatases - genetics</subject><subject>Pyrophosphatases - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Thymine Nucleotides - metabolism</subject><subject>VIRUS ARTHRITE ENCEPHALITE CAPRINE</subject><subject>VIRUS ARTRITIS ENCEFALITIS CAPRINA</subject><subject>Virus Replication - physiology</subject><subject>VIRUS VISNA MAEDI</subject><subject>visna virus</subject><subject>Visna-maedi virus - enzymology</subject><subject>Visna-maedi virus - physiology</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkPhDyAhwoZdgm-c-LFggSoelSqBoCOxsxznZsZVEgc7GdR_X4cZVe0KL64tne9cHesQ8hZoAVDKDzcHVwhalAWUwPI0RAFK8SdkA1TJvK6heko2lJZlXjP5-zl5EeMNpVBVvDojZzIBtSw3ZPsTp95ZMzs_ZlPwE4bZYcx8l7Xb6x8mYt5i56zDcc6GZTbj_E-0ZgpuxMyMbeYP66tPhDu4sESML8mzzvQRX53uc7L98vn64lt-9f3r5cWnq9xyKua8RKis4BWzjWqA20py2hpVcWw7Xla2pYILBZZ3HKS0vMFaMIaiZcIoKxp2Tj4e905LM2BrU4Rgep2iDSbcam-cfqyMbq93_qBBKsVo8r8_-YP_s2Cc9eCixb43I_olaiFULSRl_wWBq3RAJlAcQRt8jAG7-zBA9dqcTs1pQXWp1-bWIfTaXHK-efiXe9-pqqS_O-p7t9v_dQG1icPjbYl5fWQ647XZBRf19pfiAJIqdgenratH</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Turelli, P. (INSERM, Marseille, France.)</creator><creator>Petursson, G</creator><creator>Guiguen, F</creator><creator>Mornex, J.F</creator><creator>Vigne, R</creator><creator>Querat, G</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960201</creationdate><title>Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses</title><author>Turelli, P. (INSERM, Marseille, France.) ; Petursson, G ; Guiguen, F ; Mornex, J.F ; Vigne, R ; Querat, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-2e14c7643cb9b16c4860da946edf624cd076791c6f6188c6be5733e7d37a9c7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Animals</topic><topic>Arthritis-Encephalitis Virus, Caprine - enzymology</topic><topic>Arthritis-Encephalitis Virus, Caprine - physiology</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>DNA, Viral</topic><topic>HIDROLASAS</topic><topic>Humans</topic><topic>HYDROLASE</topic><topic>MACROFAGOS</topic><topic>MACROPHAGE</topic><topic>Macrophages - cytology</topic><topic>Molecular Sequence Data</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Mutation</topic><topic>Pyrophosphatases - genetics</topic><topic>Pyrophosphatases - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Thymine Nucleotides - metabolism</topic><topic>VIRUS ARTHRITE ENCEPHALITE CAPRINE</topic><topic>VIRUS ARTRITIS ENCEFALITIS CAPRINA</topic><topic>Virus Replication - physiology</topic><topic>VIRUS VISNA MAEDI</topic><topic>visna virus</topic><topic>Visna-maedi virus - enzymology</topic><topic>Visna-maedi virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turelli, P. (INSERM, Marseille, France.)</creatorcontrib><creatorcontrib>Petursson, G</creatorcontrib><creatorcontrib>Guiguen, F</creatorcontrib><creatorcontrib>Mornex, J.F</creatorcontrib><creatorcontrib>Vigne, R</creatorcontrib><creatorcontrib>Querat, G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turelli, P. (INSERM, Marseille, France.)</au><au>Petursson, G</au><au>Guiguen, F</au><au>Mornex, J.F</au><au>Vigne, R</au><au>Querat, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>70</volume><issue>2</issue><spage>1213</spage><epage>1217</epage><pages>1213-1217</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase-defective as a result of a single amino acid substitution. 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| ispartof | Journal of Virology, 1996-02, Vol.70 (2), p.1213-1217 |
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| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Animals Arthritis-Encephalitis Virus, Caprine - enzymology Arthritis-Encephalitis Virus, Caprine - physiology Base Sequence Cells, Cultured DNA, Viral HIDROLASAS Humans HYDROLASE MACROFAGOS MACROPHAGE Macrophages - cytology Molecular Sequence Data MUTANT MUTANTES Mutation Pyrophosphatases - genetics Pyrophosphatases - metabolism Sequence Homology, Amino Acid Thymine Nucleotides - metabolism VIRUS ARTHRITE ENCEPHALITE CAPRINE VIRUS ARTRITIS ENCEFALITIS CAPRINA Virus Replication - physiology VIRUS VISNA MAEDI visna virus Visna-maedi virus - enzymology Visna-maedi virus - physiology |
| title | Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses |
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