Antibacterial and cytotoxic effects of N-butyl-2-cyanoacrylate used as a tissue adhesive

In this study, the antibacterial and cytotoxic effects of a synthetic tissue adhesive called n-butyl-2-cyanoacrylate (Histoacryl) which provide rapid healing in incision in oral surgical procedures were investigated. The antibacterial effects of Histoacryl were tested by using standard reference strains of Streptococcus pyogenes, Streptococcus mitis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus grown in Mueller-Hinton media (sheep blood supplemented Mueller-Hinton for streptococcus strains) by diffusion technique. The cytotoxic effects were also researched on continuous Vero and primary gingival fibroblast cell cultures by using agar overlay method. In these methods, the inhibition zones produced by Histoacryl were evaluated. As a result, Histoacryl was found to have antibacterial effect on the tested bacteria and produce cytotoxic and cytopathic effects on the tested cell cultures.