Bioburden and theoretical sterility dose calculation for radiation sterilization of surgical cotton and bandages
The initial bioburden, count of colony forming unit (CFU) was determined on the locally manufactured non-sterilized surgical cotton and bandages. In all 489 tests were conducted on 163 cotton samples and 246 tests on 82 bandage samples. The surgical cotton showed an average of 198 microbes with a ma...
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Veröffentlicht in: | Journal of the Pakistan Medical Association 1993, Vol.43 (1), p.8-10 |
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description | The initial bioburden, count of colony forming unit (CFU) was determined on the locally manufactured non-sterilized surgical cotton and bandages. In all 489 tests were conducted on 163 cotton samples and 246 tests on 82 bandage samples. The surgical cotton showed an average of 198 microbes with a maxima of 287 and minima 94 whereas bandages showed an average of 179 microbes with a maxima of 268 and minima of 89. In the 20% samples subjected to identification no anaerobic microorganism was isolated while the aerobic microorganisms isolated were all bacilli. The sterilization dose (SD) for sterility assurance level (SAL) of 10(-6) was 2.23 Mrads and 2.21 Mrads, whereas the device verification dose (DVD) was 0.6 and 0.59 Mrads for cotton and bandages respectively as calculated by the method proposed by the Sterilization Standard Committee, Association of Advancement of Medical Instrumentation (AAMI). |
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In all 489 tests were conducted on 163 cotton samples and 246 tests on 82 bandage samples. The surgical cotton showed an average of 198 microbes with a maxima of 287 and minima 94 whereas bandages showed an average of 179 microbes with a maxima of 268 and minima of 89. In the 20% samples subjected to identification no anaerobic microorganism was isolated while the aerobic microorganisms isolated were all bacilli. The sterilization dose (SD) for sterility assurance level (SAL) of 10(-6) was 2.23 Mrads and 2.21 Mrads, whereas the device verification dose (DVD) was 0.6 and 0.59 Mrads for cotton and bandages respectively as calculated by the method proposed by the Sterilization Standard Committee, Association of Advancement of Medical Instrumentation (AAMI).</description><identifier>ISSN: 0030-9982</identifier><identifier>PMID: 8474221</identifier><identifier>CODEN: JJPAD4</identifier><language>eng</language><publisher>Karachi: Pakistan Medical Association</publisher><subject>Bacteria - growth & development ; Bacteria - radiation effects ; Bandages ; Biological and medical sciences ; Collective facilities ; Colony Count, Microbial ; Environment. Living conditions ; Equipment Contamination ; Gossypium - microbiology ; Medical sciences ; Public health. Hygiene ; Public health. Hygiene-occupational medicine ; Radiation Dosage ; Sterilization - methods ; Tropical medicine</subject><ispartof>Journal of the Pakistan Medical Association, 1993, Vol.43 (1), p.8-10</ispartof><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4653939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8474221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JAVAID KHURSHID, S</creatorcontrib><title>Bioburden and theoretical sterility dose calculation for radiation sterilization of surgical cotton and bandages</title><title>Journal of the Pakistan Medical Association</title><addtitle>J Pak Med Assoc</addtitle><description>The initial bioburden, count of colony forming unit (CFU) was determined on the locally manufactured non-sterilized surgical cotton and bandages. In all 489 tests were conducted on 163 cotton samples and 246 tests on 82 bandage samples. The surgical cotton showed an average of 198 microbes with a maxima of 287 and minima 94 whereas bandages showed an average of 179 microbes with a maxima of 268 and minima of 89. In the 20% samples subjected to identification no anaerobic microorganism was isolated while the aerobic microorganisms isolated were all bacilli. The sterilization dose (SD) for sterility assurance level (SAL) of 10(-6) was 2.23 Mrads and 2.21 Mrads, whereas the device verification dose (DVD) was 0.6 and 0.59 Mrads for cotton and bandages respectively as calculated by the method proposed by the Sterilization Standard Committee, Association of Advancement of Medical Instrumentation (AAMI).</description><subject>Bacteria - growth & development</subject><subject>Bacteria - radiation effects</subject><subject>Bandages</subject><subject>Biological and medical sciences</subject><subject>Collective facilities</subject><subject>Colony Count, Microbial</subject><subject>Environment. Living conditions</subject><subject>Equipment Contamination</subject><subject>Gossypium - microbiology</subject><subject>Medical sciences</subject><subject>Public health. Hygiene</subject><subject>Public health. Hygiene-occupational medicine</subject><subject>Radiation Dosage</subject><subject>Sterilization - methods</subject><subject>Tropical medicine</subject><issn>0030-9982</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j8tqwzAQRbVoSdOkn1DQolvDWC9byzb0BYFumnXQM1VxLCPJi_Tra2pTBma4cy-HmSu0BqBQSdmSG3Sb8zcAERxghVYtaxgh9RoNTyHqMVnXY9VbXL5cTK4Eozqci0uhC-WCbcwOTyszdqqE2GMfE07KhlktwZ9ZRY_zmE5_CBNLiTNZT02dXN6ia6-67O6WuUGHl-fP3Vu1_3h93z3uq6HmUCouvXZUg9dC01bUoFtDWy0ZUZRbRoS3wJqGCiGZ9dJx31jPoNFUSuIN0A26n7nDqM_OHocUzipdjsvnk_-w-CpPp_qkehPyf4wJTuVUv-peYzk</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>JAVAID KHURSHID, S</creator><general>Pakistan Medical Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>1993</creationdate><title>Bioburden and theoretical sterility dose calculation for radiation sterilization of surgical cotton and bandages</title><author>JAVAID KHURSHID, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p150t-59fbe3b0fb6b38610b8c38b942a35d426fd047736694df9e5f7df407b3992fc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteria - growth & development</topic><topic>Bacteria - radiation effects</topic><topic>Bandages</topic><topic>Biological and medical sciences</topic><topic>Collective facilities</topic><topic>Colony Count, Microbial</topic><topic>Environment. Living conditions</topic><topic>Equipment Contamination</topic><topic>Gossypium - microbiology</topic><topic>Medical sciences</topic><topic>Public health. Hygiene</topic><topic>Public health. Hygiene-occupational medicine</topic><topic>Radiation Dosage</topic><topic>Sterilization - methods</topic><topic>Tropical medicine</topic><toplevel>online_resources</toplevel><creatorcontrib>JAVAID KHURSHID, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of the Pakistan Medical Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JAVAID KHURSHID, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioburden and theoretical sterility dose calculation for radiation sterilization of surgical cotton and bandages</atitle><jtitle>Journal of the Pakistan Medical Association</jtitle><addtitle>J Pak Med Assoc</addtitle><date>1993</date><risdate>1993</risdate><volume>43</volume><issue>1</issue><spage>8</spage><epage>10</epage><pages>8-10</pages><issn>0030-9982</issn><coden>JJPAD4</coden><abstract>The initial bioburden, count of colony forming unit (CFU) was determined on the locally manufactured non-sterilized surgical cotton and bandages. In all 489 tests were conducted on 163 cotton samples and 246 tests on 82 bandage samples. The surgical cotton showed an average of 198 microbes with a maxima of 287 and minima 94 whereas bandages showed an average of 179 microbes with a maxima of 268 and minima of 89. In the 20% samples subjected to identification no anaerobic microorganism was isolated while the aerobic microorganisms isolated were all bacilli. The sterilization dose (SD) for sterility assurance level (SAL) of 10(-6) was 2.23 Mrads and 2.21 Mrads, whereas the device verification dose (DVD) was 0.6 and 0.59 Mrads for cotton and bandages respectively as calculated by the method proposed by the Sterilization Standard Committee, Association of Advancement of Medical Instrumentation (AAMI).</abstract><cop>Karachi</cop><pub>Pakistan Medical Association</pub><pmid>8474221</pmid><tpages>3</tpages></addata></record> |
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subjects | Bacteria - growth & development Bacteria - radiation effects Bandages Biological and medical sciences Collective facilities Colony Count, Microbial Environment. Living conditions Equipment Contamination Gossypium - microbiology Medical sciences Public health. Hygiene Public health. Hygiene-occupational medicine Radiation Dosage Sterilization - methods Tropical medicine |
title | Bioburden and theoretical sterility dose calculation for radiation sterilization of surgical cotton and bandages |
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