In Vitro Toxicity of Biomaterials Determined with Cell Density, Total Protein, Cell Cycle Distribution and Adenine Nucleotides
Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Po...
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| Veröffentlicht in: | Artificial cells, blood substitutes, and immobilization biotechnology blood substitutes, and immobilization biotechnology, 1993, Vol.21 (1), p.63-70 |
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| container_title | Artificial cells, blood substitutes, and immobilization biotechnology |
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| creator | Wieslander, Anders P. Nordin, Marika K. Hansson, Björn Baldetorp, Bo Kjellstrand, Per T.T. |
| description | Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity. |
| doi_str_mv | 10.3109/10731199309118297 |
| format | Article |
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The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity.</description><identifier>ISSN: 1073-1199</identifier><identifier>ISSN: 1055-7172</identifier><identifier>EISSN: 1532-4184</identifier><identifier>DOI: 10.3109/10731199309118297</identifier><identifier>PMID: 8461437</identifier><language>eng</language><publisher>New York, NY: Informa UK Ltd</publisher><subject>Adenine Nucleotides - analysis ; Biocompatible Materials - toxicity ; Biological and medical sciences ; Cancer and Oncology ; Cancer och onkologi ; Cell Count - drug effects ; Cell Cycle - drug effects ; Cell Line - drug effects ; Clinical Medicine ; Energy Metabolism - drug effects ; In Vitro Techniques ; Klinisk medicin ; Medical and Health Sciences ; Medical sciences ; Medicin och hälsovetenskap ; Polyvinyl Chloride - toxicity ; Proteins - analysis ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Resins, Synthetic - toxicity ; Technology. Biomaterials. Equipments. Material. Instrumentation</subject><ispartof>Artificial cells, blood substitutes, and immobilization biotechnology, 1993, Vol.21 (1), p.63-70</ispartof><rights>1993 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1993</rights><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476a-4454cf89afdea3bc35622dcf7512ea84f5fecd1f59dbe7b3d88271cdca614cf3</citedby><cites>FETCH-LOGICAL-c476a-4454cf89afdea3bc35622dcf7512ea84f5fecd1f59dbe7b3d88271cdca614cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10731199309118297$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10731199309118297$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>230,314,778,782,883,4012,27906,27907,27908,59628,60417,61202,61383</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4637746$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8461437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://lup.lub.lu.se/record/1107064$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Wieslander, Anders P.</creatorcontrib><creatorcontrib>Nordin, Marika K.</creatorcontrib><creatorcontrib>Hansson, Björn</creatorcontrib><creatorcontrib>Baldetorp, Bo</creatorcontrib><creatorcontrib>Kjellstrand, Per T.T.</creatorcontrib><title>In Vitro Toxicity of Biomaterials Determined with Cell Density, Total Protein, Cell Cycle Distribution and Adenine Nucleotides</title><title>Artificial cells, blood substitutes, and immobilization biotechnology</title><addtitle>Biomater Artif Cells Immobilization Biotechnol</addtitle><description>Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity.</description><subject>Adenine Nucleotides - analysis</subject><subject>Biocompatible Materials - toxicity</subject><subject>Biological and medical sciences</subject><subject>Cancer and Oncology</subject><subject>Cancer och onkologi</subject><subject>Cell Count - drug effects</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line - drug effects</subject><subject>Clinical Medicine</subject><subject>Energy Metabolism - drug effects</subject><subject>In Vitro Techniques</subject><subject>Klinisk medicin</subject><subject>Medical and Health Sciences</subject><subject>Medical sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Polyvinyl Chloride - toxicity</subject><subject>Proteins - analysis</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Resins, Synthetic - toxicity</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><issn>1073-1199</issn><issn>1055-7172</issn><issn>1532-4184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUc2PEyEUnxjNulb_AA8mHIynrcLADEP0snb92KRRD41XwsAjZTMDFZjUXvzbpZnaxJishwcv_D54L7-qek7wa0qweEMwp4QIQbEgpKsFf1BdkobWS0Y69rD0BV8eCY-rJyndYYwpI_VFddGxljDKL6tftx59dzkGtAk_nXb5gIJF710YVYbo1JDQDZRudB4M2ru8RSsYhvLoUyFfFVlWA_oWQwbnr2ZwddADoBuXcnT9lF3wSHmDrg34YoO-TAUO2RlIT6tHtvwBz073otp8_LBZfV6uv366XV2vl5rxVi0Za5i2nVDWgKK9pk1b10Zb3pAaVMdsY0EbYhtheuA9NV1Xc6KNVmVNbemiWs-2aQ-7qZe76EYVDzIoJ4dpV6ovJRNIxutixFqpSS8kEw2WApNWAgjamK4FwKrYvZrtdjH8mCBlObqky-bKQ5iSrFvSiI7w_xJJy7jg_EgkM1HHkFIEex6RYHmMWv4TddG8OJlP_QjmrDhlW_CXJ1wlrQYbldcunWmspZyXY1G9m2nO2xBHtQ9xMDKrwxDiHw29b4q3f8m3oIa81SqCvAtT9CXVe3b4DbR01yM</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Wieslander, Anders P.</creator><creator>Nordin, Marika K.</creator><creator>Hansson, Björn</creator><creator>Baldetorp, Bo</creator><creator>Kjellstrand, Per T.T.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Dekker</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>JG9</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D95</scope></search><sort><creationdate>1993</creationdate><title>In Vitro Toxicity of Biomaterials Determined with Cell Density, Total Protein, Cell Cycle Distribution and Adenine Nucleotides</title><author>Wieslander, Anders P. ; Nordin, Marika K. ; Hansson, Björn ; Baldetorp, Bo ; Kjellstrand, Per T.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476a-4454cf89afdea3bc35622dcf7512ea84f5fecd1f59dbe7b3d88271cdca614cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenine Nucleotides - analysis</topic><topic>Biocompatible Materials - toxicity</topic><topic>Biological and medical sciences</topic><topic>Cancer and Oncology</topic><topic>Cancer och onkologi</topic><topic>Cell Count - drug effects</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Line - drug effects</topic><topic>Clinical Medicine</topic><topic>Energy Metabolism - drug effects</topic><topic>In Vitro Techniques</topic><topic>Klinisk medicin</topic><topic>Medical and Health Sciences</topic><topic>Medical sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Polyvinyl Chloride - toxicity</topic><topic>Proteins - analysis</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Resins, Synthetic - toxicity</topic><topic>Technology. Biomaterials. Equipments. Material. 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The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. 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| ispartof | Artificial cells, blood substitutes, and immobilization biotechnology, 1993, Vol.21 (1), p.63-70 |
| issn | 1073-1199 1055-7172 1532-4184 |
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| source | MEDLINE; Taylor & Francis |
| subjects | Adenine Nucleotides - analysis Biocompatible Materials - toxicity Biological and medical sciences Cancer and Oncology Cancer och onkologi Cell Count - drug effects Cell Cycle - drug effects Cell Line - drug effects Clinical Medicine Energy Metabolism - drug effects In Vitro Techniques Klinisk medicin Medical and Health Sciences Medical sciences Medicin och hälsovetenskap Polyvinyl Chloride - toxicity Proteins - analysis Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Resins, Synthetic - toxicity Technology. Biomaterials. Equipments. Material. Instrumentation |
| title | In Vitro Toxicity of Biomaterials Determined with Cell Density, Total Protein, Cell Cycle Distribution and Adenine Nucleotides |
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