Gamma interferon cooperates with lipopolysaccharide to activate mouse splenic macrophages to an antihistoplasma state
Inhibition of the intracellular growth of Histoplasma capsulatum by murine resident red pulp splenic macrophages was examined. Splenic macrophages, unlike resident peritoneal macrophages, required a prolonged preincubation (18 h) with recombinant murine gamma interferon (rMuIFN-gamma) for activation...
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| Veröffentlicht in: | Infection and Immunity 1993-04, Vol.61 (4), p.1468-1473 |
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| creator | Lane, T.E Wu-Hsieh, B.A Howard, D.H |
| description | Inhibition of the intracellular growth of Histoplasma capsulatum by murine resident red pulp splenic macrophages was examined. Splenic macrophages, unlike resident peritoneal macrophages, required a prolonged preincubation (18 h) with recombinant murine gamma interferon (rMuIFN-gamma) for activation. To be fully activated, the splenic macrophages required incubation with rMuIFN-gamma in combination with 0.1 micrograms of lipopolysaccharide (LPS) per ml. Splenic macrophages stimulated with rMuIFN-gamma, LPS, or rMuIFN-gamma and LPS produced tumor necrosis factor alpha (TNF-alpha), but recombinant murine TNF-alpha (rMuTNF-alpha) did not activate macrophages when used alone or as a second signal with rMuIFN-gamma. Anti-TNF-alpha antibody did not block IFN-gamma-LPS activation of splenic macrophages to any significant extent. One hundred micromolar ferrous sulfate antagonized IFN-gamma-LPS activation of splenic macrophages, indicating that iron was involved in the fungistatic activity of cytokine-stimulated phagocytes. Our results indicate that (i) splenic macrophages differ significantly from peritoneal macrophages in their requirements for activation and (ii) the mechanism by which splenic macrophages exert their antifungal effects involves iron |
| doi_str_mv | 10.1128/IAI.61.4.1468-1473.1993 |
| format | Article |
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Splenic macrophages, unlike resident peritoneal macrophages, required a prolonged preincubation (18 h) with recombinant murine gamma interferon (rMuIFN-gamma) for activation. To be fully activated, the splenic macrophages required incubation with rMuIFN-gamma in combination with 0.1 micrograms of lipopolysaccharide (LPS) per ml. Splenic macrophages stimulated with rMuIFN-gamma, LPS, or rMuIFN-gamma and LPS produced tumor necrosis factor alpha (TNF-alpha), but recombinant murine TNF-alpha (rMuTNF-alpha) did not activate macrophages when used alone or as a second signal with rMuIFN-gamma. Anti-TNF-alpha antibody did not block IFN-gamma-LPS activation of splenic macrophages to any significant extent. One hundred micromolar ferrous sulfate antagonized IFN-gamma-LPS activation of splenic macrophages, indicating that iron was involved in the fungistatic activity of cytokine-stimulated phagocytes. Our results indicate that (i) splenic macrophages differ significantly from peritoneal macrophages in their requirements for activation and (ii) the mechanism by which splenic macrophages exert their antifungal effects involves iron</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.61.4.1468-1473.1993</identifier><identifier>PMID: 8454351</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Biological and medical sciences ; Drug Synergism ; FAGOCITOS ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; HISTOPLASMA ; Histoplasma - immunology ; Histoplasmosis - immunology ; Immunobiology ; Interferon-gamma - administration & dosage ; Iron - pharmacology ; Lipopolysaccharides - administration & dosage ; Macrophage Activation - drug effects ; Macrophages - immunology ; Male ; Mice ; Mice, Inbred C57BL ; Monocytes, macrophages ; Myeloid cells: ontogeny, maturation, markers, receptors ; PHAGOCYTE ; RATON ; Recombinant Proteins ; SOURIS ; Spleen - cytology ; Spleen - immunology ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Infection and Immunity, 1993-04, Vol.61 (4), p.1468-1473</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-fd539b774222d83b5354147544d47d413053ab3ae0541682918ad24909fa8b7c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC281387/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC281387/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4717725$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8454351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lane, T.E</creatorcontrib><creatorcontrib>Wu-Hsieh, B.A</creatorcontrib><creatorcontrib>Howard, D.H</creatorcontrib><title>Gamma interferon cooperates with lipopolysaccharide to activate mouse splenic macrophages to an antihistoplasma state</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Inhibition of the intracellular growth of Histoplasma capsulatum by murine resident red pulp splenic macrophages was examined. Splenic macrophages, unlike resident peritoneal macrophages, required a prolonged preincubation (18 h) with recombinant murine gamma interferon (rMuIFN-gamma) for activation. To be fully activated, the splenic macrophages required incubation with rMuIFN-gamma in combination with 0.1 micrograms of lipopolysaccharide (LPS) per ml. Splenic macrophages stimulated with rMuIFN-gamma, LPS, or rMuIFN-gamma and LPS produced tumor necrosis factor alpha (TNF-alpha), but recombinant murine TNF-alpha (rMuTNF-alpha) did not activate macrophages when used alone or as a second signal with rMuIFN-gamma. Anti-TNF-alpha antibody did not block IFN-gamma-LPS activation of splenic macrophages to any significant extent. One hundred micromolar ferrous sulfate antagonized IFN-gamma-LPS activation of splenic macrophages, indicating that iron was involved in the fungistatic activity of cytokine-stimulated phagocytes. Our results indicate that (i) splenic macrophages differ significantly from peritoneal macrophages in their requirements for activation and (ii) the mechanism by which splenic macrophages exert their antifungal effects involves iron</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Drug Synergism</subject><subject>FAGOCITOS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HISTOPLASMA</subject><subject>Histoplasma - immunology</subject><subject>Histoplasmosis - immunology</subject><subject>Immunobiology</subject><subject>Interferon-gamma - administration & dosage</subject><subject>Iron - pharmacology</subject><subject>Lipopolysaccharides - administration & dosage</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Monocytes, macrophages</subject><subject>Myeloid cells: ontogeny, maturation, markers, receptors</subject><subject>PHAGOCYTE</subject><subject>RATON</subject><subject>Recombinant Proteins</subject><subject>SOURIS</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV-L1DAUxYso67j6BQSxgvjW2vxrkod9WBZdBxZ80H0Od9J0GmmbmmR22W_vLTMMLgRCcn_n3pycovhImpoQqr5ur7d1S2peE96qinDJaqI1e1FsSKNVJQSlL4tN0xBdadHK18WblP7gkXOuLooLxQVngmyKwy1ME5R-zi72Loa5tCEsLkJ2qXz0eShHv4QljE8JrB0g-s6VOZRgs39AqJzCIbkyLaObvS0nsDEsA-xRvVIzruwHn3JYRkg4KWVUvS1e9TAm9-60Xxb337_9vvlR3f283d5c31VWyCZXfSeY3knJKaWdYjvBBEergvOOy44T1ggGOwauwftWUU0UdJTrRvegdtKyy-Lq2Hc57CbXWTfnCKNZop8gPpkA3jyvzH4w-_BgqCJMSdR_Oelj-HtwKZvJJ-vGEWaHvg1pmRCSagTlEUT7KUXXn2eQxqyBGY-zWmK4WQMza2BmDQyVH_5_4ll3Sgjrn091SBbGPsJsfTpjXBIpqUDs0xEb_H549NEZ_O3nQ5F5f2R6CAb2Edvc_9KcoNGW_QMuvLVt</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>Lane, T.E</creator><creator>Wu-Hsieh, B.A</creator><creator>Howard, D.H</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>19930401</creationdate><title>Gamma interferon cooperates with lipopolysaccharide to activate mouse splenic macrophages to an antihistoplasma state</title><author>Lane, T.E ; Wu-Hsieh, B.A ; Howard, D.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-fd539b774222d83b5354147544d47d413053ab3ae0541682918ad24909fa8b7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Drug Synergism</topic><topic>FAGOCITOS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>HISTOPLASMA</topic><topic>Histoplasma - immunology</topic><topic>Histoplasmosis - immunology</topic><topic>Immunobiology</topic><topic>Interferon-gamma - administration & dosage</topic><topic>Iron - pharmacology</topic><topic>Lipopolysaccharides - administration & dosage</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Monocytes, macrophages</topic><topic>Myeloid cells: ontogeny, maturation, markers, receptors</topic><topic>PHAGOCYTE</topic><topic>RATON</topic><topic>Recombinant Proteins</topic><topic>SOURIS</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lane, T.E</creatorcontrib><creatorcontrib>Wu-Hsieh, B.A</creatorcontrib><creatorcontrib>Howard, D.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lane, T.E</au><au>Wu-Hsieh, B.A</au><au>Howard, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gamma interferon cooperates with lipopolysaccharide to activate mouse splenic macrophages to an antihistoplasma state</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>61</volume><issue>4</issue><spage>1468</spage><epage>1473</epage><pages>1468-1473</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>Inhibition of the intracellular growth of Histoplasma capsulatum by murine resident red pulp splenic macrophages was examined. Splenic macrophages, unlike resident peritoneal macrophages, required a prolonged preincubation (18 h) with recombinant murine gamma interferon (rMuIFN-gamma) for activation. To be fully activated, the splenic macrophages required incubation with rMuIFN-gamma in combination with 0.1 micrograms of lipopolysaccharide (LPS) per ml. Splenic macrophages stimulated with rMuIFN-gamma, LPS, or rMuIFN-gamma and LPS produced tumor necrosis factor alpha (TNF-alpha), but recombinant murine TNF-alpha (rMuTNF-alpha) did not activate macrophages when used alone or as a second signal with rMuIFN-gamma. Anti-TNF-alpha antibody did not block IFN-gamma-LPS activation of splenic macrophages to any significant extent. One hundred micromolar ferrous sulfate antagonized IFN-gamma-LPS activation of splenic macrophages, indicating that iron was involved in the fungistatic activity of cytokine-stimulated phagocytes. Our results indicate that (i) splenic macrophages differ significantly from peritoneal macrophages in their requirements for activation and (ii) the mechanism by which splenic macrophages exert their antifungal effects involves iron</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8454351</pmid><doi>10.1128/IAI.61.4.1468-1473.1993</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Biological and medical sciences Drug Synergism FAGOCITOS Fundamental and applied biological sciences. Psychology Fundamental immunology HISTOPLASMA Histoplasma - immunology Histoplasmosis - immunology Immunobiology Interferon-gamma - administration & dosage Iron - pharmacology Lipopolysaccharides - administration & dosage Macrophage Activation - drug effects Macrophages - immunology Male Mice Mice, Inbred C57BL Monocytes, macrophages Myeloid cells: ontogeny, maturation, markers, receptors PHAGOCYTE RATON Recombinant Proteins SOURIS Spleen - cytology Spleen - immunology Tumor Necrosis Factor-alpha - metabolism |
| title | Gamma interferon cooperates with lipopolysaccharide to activate mouse splenic macrophages to an antihistoplasma state |
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