Transcription Factor TFIID Recognizes DNA Sequences Downstream of the TATA Element in the Hsp70 Heat Shock Gene
The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibod...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-09, Vol.90 (18), p.8449-8453 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Emanuel, Peter A. Gilmour, David S. |
| description | The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters. |
| doi_str_mv | 10.1073/pnas.90.18.8449 |
| format | Article |
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Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.18.8449</identifier><identifier>PMID: 8378317</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Base Sequence ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; DNA - genetics ; DNA - isolation & purification ; DNA - metabolism ; DNA-Binding Proteins - isolation & purification ; DNA-Binding Proteins - metabolism ; Drosophila ; Drosophila - genetics ; Drosophila - metabolism ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Gels ; Genes ; Genetic mutation ; Heat-Shock Proteins - genetics ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Nucleotide sequences ; Promoter regions ; Promoter Regions, Genetic ; Recombinant Proteins - metabolism ; Restriction Mapping ; Sequence Deletion ; Shock heating ; Substrate Specificity ; TATA Box ; TATA-Box Binding Protein ; Transcription Factor TFIID ; Transcription Factors - isolation & purification ; Transcription Factors - metabolism ; Transcription initiation site ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-09, Vol.90 (18), p.8449-8453</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Sep 15, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-db979bf473c42c650034bb6454365bf48a9635a0e7d7a136482615e994d7b90b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2362867$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2362867$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27922,27923,53789,53791,58015,58248</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3765692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8378317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Emanuel, Peter A.</creatorcontrib><creatorcontrib>Gilmour, David S.</creatorcontrib><title>Transcription Factor TFIID Recognizes DNA Sequences Downstream of the TATA Element in the Hsp70 Heat Shock Gene</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA - metabolism</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Nucleotide sequences</subject><subject>Promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Proteins - metabolism</subject><subject>Restriction Mapping</subject><subject>Sequence Deletion</subject><subject>Shock heating</subject><subject>Substrate Specificity</subject><subject>TATA Box</subject><subject>TATA-Box Binding Protein</subject><subject>Transcription Factor TFIID</subject><subject>Transcription Factors - isolation & purification</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription initiation site</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstv1DAQxiMEKkvhzAWQhRA9ZevEjh8Sl1Xb7a5UgUTD2XK8TjdLYqe2w-uvx-mmy-MAJ2vm-41nxp-T5HkG5xmk6LQ30s95DNicYcwfJLMM8iwlmMOHyQzCnKYM5_hx8sT7HYSQFwweJUcMUYYyOkts6aTxyjV9aKwBS6mCdaBcrtfn4KNW9sY0P7QH5-8X4FrfDtqoMbJfjQ9Oyw7YGoStBuWiXICLVnfaBNCYu9zK9xSClZYBXG-t-gwutdFPk0e1bL1-Np3HyaflRXm2Sq8-XK7PFlepKjAP6abilFc1pkjhXJECQoSriuACI1LEPJOcoEJCTTdUZohglpOs0JzjDa04rNBx8m5_bz9Und6oOJeTrehd00n3XVjZiD8V02zFjf0iYkuKY_nbqdzZuLUPomu80m0rjbaDF7TgFDKe_xfMCCkI4yyCr_8Cd3ZwJr6ByGGWI8rpCJ3uIeWs907Xh4EzKEa_xei34DFgYvQ7Vrz8fc8DPxkc9TeTLr2SbR3dVo0_YIjG8e7WOJmw8f579VcfUQ9tG_S3EMlX_yQj8GIP7Hz8SgciRyRnhKKf3YXTMQ</recordid><startdate>19930915</startdate><enddate>19930915</enddate><creator>Emanuel, Peter A.</creator><creator>Gilmour, David S.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930915</creationdate><title>Transcription Factor TFIID Recognizes DNA Sequences Downstream of the TATA Element in the Hsp70 Heat Shock Gene</title><author>Emanuel, Peter A. ; Gilmour, David S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-db979bf473c42c650034bb6454365bf48a9635a0e7d7a136482615e994d7b90b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA - metabolism</topic><topic>DNA-Binding Proteins - isolation & purification</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Drosophila</topic><topic>Drosophila - genetics</topic><topic>Drosophila - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genes</topic><topic>Genetic mutation</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Nucleotide sequences</topic><topic>Promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Proteins - metabolism</topic><topic>Restriction Mapping</topic><topic>Sequence Deletion</topic><topic>Shock heating</topic><topic>Substrate Specificity</topic><topic>TATA Box</topic><topic>TATA-Box Binding Protein</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors - isolation & purification</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription initiation site</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Emanuel, Peter A.</creatorcontrib><creatorcontrib>Gilmour, David S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Emanuel, Peter A.</au><au>Gilmour, David S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription Factor TFIID Recognizes DNA Sequences Downstream of the TATA Element in the Hsp70 Heat Shock Gene</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-09-15</date><risdate>1993</risdate><volume>90</volume><issue>18</issue><spage>8449</spage><epage>8453</epage><pages>8449-8453</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8378317</pmid><doi>10.1073/pnas.90.18.8449</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1993-09, Vol.90 (18), p.8449-8453 |
| issn | 0027-8424 1091-6490 |
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| recordid | cdi_pubmed_primary_8378317 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Base Sequence Biological and medical sciences Deoxyribonucleic acid DNA DNA - genetics DNA - isolation & purification DNA - metabolism DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Drosophila Drosophila - genetics Drosophila - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gels Genes Genetic mutation Heat-Shock Proteins - genetics Molecular and cellular biology Molecular genetics Mutation Nucleotide sequences Promoter regions Promoter Regions, Genetic Recombinant Proteins - metabolism Restriction Mapping Sequence Deletion Shock heating Substrate Specificity TATA Box TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors - isolation & purification Transcription Factors - metabolism Transcription initiation site Transcription. Transcription factor. Splicing. Rna processing |
| title | Transcription Factor TFIID Recognizes DNA Sequences Downstream of the TATA Element in the Hsp70 Heat Shock Gene |
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