Characterization of a Saporin Mitotoxin Specifically Cytotoxic to Cells Bearing the Granulocyte-Macrophage Colony-Stimulating Factor Receptor
Abstract When granulocyte-macrophage colony-stimulating factor (GM-CSF) is chemically conjugated to the ribosome-inactivating protein saporin, the resulting protein conjugate is highly toxic for cells expressing the GM-CSF receptor. Structural and Western blot analyses of the purified conjugate esta...
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| Veröffentlicht in: | Growth factors (Chur, Switzerland) Switzerland), 1993, Vol.9 (1), p.31-39 |
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| container_title | Growth factors (Chur, Switzerland) |
| container_volume | 9 |
| creator | Lappi, Douglas A. Martineau, Darlene Sarmientos, Paolo Garofano, Luisa Aranda, Augustin Perez Miyajima, Atsushi Kitamura, Toshio Baird, Andrew |
| description | Abstract
When granulocyte-macrophage colony-stimulating factor (GM-CSF) is chemically conjugated to the ribosome-inactivating protein saporin, the resulting protein conjugate is highly toxic for cells expressing the GM-CSF receptor. Structural and Western blot analyses of the purified conjugate establish that it contains equimolar amounts of the starting materials and is free of any contamination by the non-conjugated components. The resulting bifunctional reagent is specifically cytotoxic to cells expressing the GM-CSF receptor, but is ineffective to cells that do not express the receptor. The cytotoxic activity is inhibited in a dose-dependent manner by GM-CSF, but not by any one of five other peptide growth factors. This is the first report of a mitotoxin for cells that express the GM-CSF receptor and which promises to be a valuable tool to study the expression of the GM-CSF receptor in normal and pathological states. |
| doi_str_mv | 10.3109/08977199308991580 |
| format | Article |
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When granulocyte-macrophage colony-stimulating factor (GM-CSF) is chemically conjugated to the ribosome-inactivating protein saporin, the resulting protein conjugate is highly toxic for cells expressing the GM-CSF receptor. Structural and Western blot analyses of the purified conjugate establish that it contains equimolar amounts of the starting materials and is free of any contamination by the non-conjugated components. The resulting bifunctional reagent is specifically cytotoxic to cells expressing the GM-CSF receptor, but is ineffective to cells that do not express the receptor. The cytotoxic activity is inhibited in a dose-dependent manner by GM-CSF, but not by any one of five other peptide growth factors. This is the first report of a mitotoxin for cells that express the GM-CSF receptor and which promises to be a valuable tool to study the expression of the GM-CSF receptor in normal and pathological states.</description><identifier>ISSN: 0897-7194</identifier><identifier>EISSN: 1029-2292</identifier><identifier>DOI: 10.3109/08977199308991580</identifier><identifier>PMID: 8347350</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; antagonist ; Cell Death - drug effects ; Cell Line ; Cross-Linking Reagents - administration & dosage ; Cross-Linking Reagents - isolation & purification ; granulocyte-macrophage colony-stimulating factor ; Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; immunotoxin ; Immunotoxins ; Mice ; Mitogens - administration & dosage ; mitotoxin ; N-Glycosyl Hydrolases ; Plant Proteins - administration & dosage ; Plant Proteins - toxicity ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - drug effects ; Ribosome Inactivating Proteins, Type 1 ; Ribosomes - drug effects ; saporin</subject><ispartof>Growth factors (Chur, Switzerland), 1993, Vol.9 (1), p.31-39</ispartof><rights>1993 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-66400e0202acbf0892461d181536a3f82b5e49127ae4427efa3fa78a0f7406ca3</citedby><cites>FETCH-LOGICAL-c401t-66400e0202acbf0892461d181536a3f82b5e49127ae4427efa3fa78a0f7406ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/08977199308991580$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/08977199308991580$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,4009,27902,27903,27904,59624,59730,60413,60519,61198,61233,61379,61414</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8347350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lappi, Douglas A.</creatorcontrib><creatorcontrib>Martineau, Darlene</creatorcontrib><creatorcontrib>Sarmientos, Paolo</creatorcontrib><creatorcontrib>Garofano, Luisa</creatorcontrib><creatorcontrib>Aranda, Augustin Perez</creatorcontrib><creatorcontrib>Miyajima, Atsushi</creatorcontrib><creatorcontrib>Kitamura, Toshio</creatorcontrib><creatorcontrib>Baird, Andrew</creatorcontrib><title>Characterization of a Saporin Mitotoxin Specifically Cytotoxic to Cells Bearing the Granulocyte-Macrophage Colony-Stimulating Factor Receptor</title><title>Growth factors (Chur, Switzerland)</title><addtitle>Growth Factors</addtitle><description>Abstract
When granulocyte-macrophage colony-stimulating factor (GM-CSF) is chemically conjugated to the ribosome-inactivating protein saporin, the resulting protein conjugate is highly toxic for cells expressing the GM-CSF receptor. Structural and Western blot analyses of the purified conjugate establish that it contains equimolar amounts of the starting materials and is free of any contamination by the non-conjugated components. The resulting bifunctional reagent is specifically cytotoxic to cells expressing the GM-CSF receptor, but is ineffective to cells that do not express the receptor. The cytotoxic activity is inhibited in a dose-dependent manner by GM-CSF, but not by any one of five other peptide growth factors. This is the first report of a mitotoxin for cells that express the GM-CSF receptor and which promises to be a valuable tool to study the expression of the GM-CSF receptor in normal and pathological states.</description><subject>Animals</subject><subject>antagonist</subject><subject>Cell Death - drug effects</subject><subject>Cell Line</subject><subject>Cross-Linking Reagents - administration & dosage</subject><subject>Cross-Linking Reagents - isolation & purification</subject><subject>granulocyte-macrophage colony-stimulating factor</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>immunotoxin</subject><subject>Immunotoxins</subject><subject>Mice</subject><subject>Mitogens - administration & dosage</subject><subject>mitotoxin</subject><subject>N-Glycosyl Hydrolases</subject><subject>Plant Proteins - administration & dosage</subject><subject>Plant Proteins - toxicity</subject><subject>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - drug effects</subject><subject>Ribosome Inactivating Proteins, Type 1</subject><subject>Ribosomes - drug effects</subject><subject>saporin</subject><issn>0897-7194</issn><issn>1029-2292</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UNtKxDAQDaLoevkAH4T8QDVJs72gL1pcFRTB1ecym53YSLYpaRat_-A_m2VFENGnOcyZc2bmEHLI2XHKWXnCijLPeVmmEZR8XLANMuJMlIkQpdgkoxWfxAG5Q3b7_oUxnsqcb5PtItZ0zEbko2rAgwrozTsE41rqNAU6hc5509I7E1xwbxFNO1RGGwXWDrQa1m1Fg6MVWtvTC4QoeKahQXrloV1ap4aAyR0o77oGnpFWzrp2SKbBLJY2LovTk7jaefqACrsI9smWBtvjwVfdI0-Ty8fqOrm9v7qpzm8TJRkPSZZJxpAJJkDNdHxSyIzPecHHaQapLsRsjLLkIgeUUuSoYxPyApjOJcsUpHuEr33jbX3vUdedNwvwQ81ZvUq2_pVs1BytNd1ytsD5t-IrysifrXnTaucX8Oq8ndcBBuu8joEo06-s_7Y__SFvEGxoFHisX9zStzGOf477BCSYm-Y</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Lappi, Douglas A.</creator><creator>Martineau, Darlene</creator><creator>Sarmientos, Paolo</creator><creator>Garofano, Luisa</creator><creator>Aranda, Augustin Perez</creator><creator>Miyajima, Atsushi</creator><creator>Kitamura, Toshio</creator><creator>Baird, Andrew</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1993</creationdate><title>Characterization of a Saporin Mitotoxin Specifically Cytotoxic to Cells Bearing the Granulocyte-Macrophage Colony-Stimulating Factor Receptor</title><author>Lappi, Douglas A. ; Martineau, Darlene ; Sarmientos, Paolo ; Garofano, Luisa ; Aranda, Augustin Perez ; Miyajima, Atsushi ; Kitamura, Toshio ; Baird, Andrew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-66400e0202acbf0892461d181536a3f82b5e49127ae4427efa3fa78a0f7406ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>antagonist</topic><topic>Cell Death - drug effects</topic><topic>Cell Line</topic><topic>Cross-Linking Reagents - administration & dosage</topic><topic>Cross-Linking Reagents - isolation & purification</topic><topic>granulocyte-macrophage colony-stimulating factor</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>immunotoxin</topic><topic>Immunotoxins</topic><topic>Mice</topic><topic>Mitogens - administration & dosage</topic><topic>mitotoxin</topic><topic>N-Glycosyl Hydrolases</topic><topic>Plant Proteins - administration & dosage</topic><topic>Plant Proteins - toxicity</topic><topic>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - drug effects</topic><topic>Ribosome Inactivating Proteins, Type 1</topic><topic>Ribosomes - drug effects</topic><topic>saporin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lappi, Douglas A.</creatorcontrib><creatorcontrib>Martineau, Darlene</creatorcontrib><creatorcontrib>Sarmientos, Paolo</creatorcontrib><creatorcontrib>Garofano, Luisa</creatorcontrib><creatorcontrib>Aranda, Augustin Perez</creatorcontrib><creatorcontrib>Miyajima, Atsushi</creatorcontrib><creatorcontrib>Kitamura, Toshio</creatorcontrib><creatorcontrib>Baird, Andrew</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Growth factors (Chur, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lappi, Douglas A.</au><au>Martineau, Darlene</au><au>Sarmientos, Paolo</au><au>Garofano, Luisa</au><au>Aranda, Augustin Perez</au><au>Miyajima, Atsushi</au><au>Kitamura, Toshio</au><au>Baird, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Saporin Mitotoxin Specifically Cytotoxic to Cells Bearing the Granulocyte-Macrophage Colony-Stimulating Factor Receptor</atitle><jtitle>Growth factors (Chur, Switzerland)</jtitle><addtitle>Growth Factors</addtitle><date>1993</date><risdate>1993</risdate><volume>9</volume><issue>1</issue><spage>31</spage><epage>39</epage><pages>31-39</pages><issn>0897-7194</issn><eissn>1029-2292</eissn><abstract>Abstract
When granulocyte-macrophage colony-stimulating factor (GM-CSF) is chemically conjugated to the ribosome-inactivating protein saporin, the resulting protein conjugate is highly toxic for cells expressing the GM-CSF receptor. Structural and Western blot analyses of the purified conjugate establish that it contains equimolar amounts of the starting materials and is free of any contamination by the non-conjugated components. The resulting bifunctional reagent is specifically cytotoxic to cells expressing the GM-CSF receptor, but is ineffective to cells that do not express the receptor. The cytotoxic activity is inhibited in a dose-dependent manner by GM-CSF, but not by any one of five other peptide growth factors. This is the first report of a mitotoxin for cells that express the GM-CSF receptor and which promises to be a valuable tool to study the expression of the GM-CSF receptor in normal and pathological states.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>8347350</pmid><doi>10.3109/08977199308991580</doi><tpages>9</tpages></addata></record> |
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| ispartof | Growth factors (Chur, Switzerland), 1993, Vol.9 (1), p.31-39 |
| issn | 0897-7194 1029-2292 |
| language | eng |
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| source | MEDLINE; Taylor & Francis; Taylor & Francis Medical Library - CRKN |
| subjects | Animals antagonist Cell Death - drug effects Cell Line Cross-Linking Reagents - administration & dosage Cross-Linking Reagents - isolation & purification granulocyte-macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology immunotoxin Immunotoxins Mice Mitogens - administration & dosage mitotoxin N-Glycosyl Hydrolases Plant Proteins - administration & dosage Plant Proteins - toxicity Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - drug effects Ribosome Inactivating Proteins, Type 1 Ribosomes - drug effects saporin |
| title | Characterization of a Saporin Mitotoxin Specifically Cytotoxic to Cells Bearing the Granulocyte-Macrophage Colony-Stimulating Factor Receptor |
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