Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI

The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1976-05, Vol.73 (5), p.1537-1541
Hauptverfasser:	D H Hamer, C A Thomas, Jr
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - enzymology DNA DNA Restriction Enzymes - metabolism DNA, Bacterial Drosophila melanogaster Endonucleases - metabolism Escherichia coli Extrachromosomal Inheritance Genetic Engineering Plasmids Streptomyces - enzymology Transformation, Genetic
Online-Zugang:	Volltext
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