Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI
The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expr...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1976-05, Vol.73 (5), p.1537-1541 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1541 |
|---|---|
| container_issue | 5 |
| container_start_page | 1537 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 73 |
| creator | D H Hamer C A Thomas, Jr |
| description | The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be made with the tetracycline resistance factor pSC101, since insertion of DNA either at its single site for Sa1I or at that for BamI inactivates plasmid-specified drug resistance but not replication. To take advantage of this insertional inactivation, pSC101 was joined to a Co1E1-ampicillin resistance plasmid having no Sa1I site, and to a Co1E1-kanamycin resistance plasmid having no BamI site. Chimeras formed with the resulting hybrid vehicles can be identified simply by replica plating. These three vehicles, which all replicate under relaxed control, have been used to clone and amplify Drosophila melanogaster DNA fragments. |
| doi_str_mv | 10.1073/pnas.73.5.1537 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_818638</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>83305475</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2837-ca29875fbbc1a56c13daaa92b5ff881d6492a0dd544cf0af5fb8826c75deffa93</originalsourceid><addsrcrecordid>eNp9kT1PHDEQhq0IEg6SNhWRXNHtxl6vb71FCsLnSQQKkrTWrD8ui7z2Ye-i8O_x6eAEDdUU8zyeGb8IfaWkpKRh31ceUtmwkpeUs-YDmlHS0mJet2QHzQipmkLUVb2H9lO6I4S0XJBP6KOgYs7EDP39FZxRk4OIlQu-90scLD69PsY2wnIwfkx4FYOelNG4e8TRpDH2auyDx8br4CflDCST8C3QBQav8U8YFp_RrgWXzJfneoD-nJ_9Prksrm4uFifHV4WqBGsKBVUrGm67TlHgc0WZBoC26ri1QlCdr6iAaM3rWlkCNpNCVHPVcG2shZYdoB-bd1dTNxit8r4RnFzFfoD4KAP08m3H9__kMjzImhHGquwfPfsx3E_5Njn0SRnnwJswJSkYI7xueAbLDahiSCkau51BiVznINc5yFy5XOeQhcPXm23xzce_GrzWXppbXdrJudH8HzP47T2QPQHD3Z3M</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83305475</pqid></control><display><type>article</type><title>Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>D H Hamer ; C A Thomas, Jr</creator><creatorcontrib>D H Hamer ; C A Thomas, Jr</creatorcontrib><description>The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be made with the tetracycline resistance factor pSC101, since insertion of DNA either at its single site for Sa1I or at that for BamI inactivates plasmid-specified drug resistance but not replication. To take advantage of this insertional inactivation, pSC101 was joined to a Co1E1-ampicillin resistance plasmid having no Sa1I site, and to a Co1E1-kanamycin resistance plasmid having no BamI site. Chimeras formed with the resulting hybrid vehicles can be identified simply by replica plating. These three vehicles, which all replicate under relaxed control, have been used to clone and amplify Drosophila melanogaster DNA fragments.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.73.5.1537</identifier><identifier>PMID: 818638</identifier><language>eng</language><publisher>United States: National Acad Sciences</publisher><subject>Bacillus - enzymology ; DNA ; DNA Restriction Enzymes - metabolism ; DNA, Bacterial ; Drosophila melanogaster ; Endonucleases - metabolism ; Escherichia coli ; Extrachromosomal Inheritance ; Genetic Engineering ; Plasmids ; Streptomyces - enzymology ; Transformation, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1976-05, Vol.73 (5), p.1537-1541</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2837-ca29875fbbc1a56c13daaa92b5ff881d6492a0dd544cf0af5fb8826c75deffa93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/73/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC430332/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC430332/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/818638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D H Hamer</creatorcontrib><creatorcontrib>C A Thomas, Jr</creatorcontrib><title>Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be made with the tetracycline resistance factor pSC101, since insertion of DNA either at its single site for Sa1I or at that for BamI inactivates plasmid-specified drug resistance but not replication. To take advantage of this insertional inactivation, pSC101 was joined to a Co1E1-ampicillin resistance plasmid having no Sa1I site, and to a Co1E1-kanamycin resistance plasmid having no BamI site. Chimeras formed with the resulting hybrid vehicles can be identified simply by replica plating. These three vehicles, which all replicate under relaxed control, have been used to clone and amplify Drosophila melanogaster DNA fragments.</description><subject>Bacillus - enzymology</subject><subject>DNA</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA, Bacterial</subject><subject>Drosophila melanogaster</subject><subject>Endonucleases - metabolism</subject><subject>Escherichia coli</subject><subject>Extrachromosomal Inheritance</subject><subject>Genetic Engineering</subject><subject>Plasmids</subject><subject>Streptomyces - enzymology</subject><subject>Transformation, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kT1PHDEQhq0IEg6SNhWRXNHtxl6vb71FCsLnSQQKkrTWrD8ui7z2Ye-i8O_x6eAEDdUU8zyeGb8IfaWkpKRh31ceUtmwkpeUs-YDmlHS0mJet2QHzQipmkLUVb2H9lO6I4S0XJBP6KOgYs7EDP39FZxRk4OIlQu-90scLD69PsY2wnIwfkx4FYOelNG4e8TRpDH2auyDx8br4CflDCST8C3QBQav8U8YFp_RrgWXzJfneoD-nJ_9Prksrm4uFifHV4WqBGsKBVUrGm67TlHgc0WZBoC26ri1QlCdr6iAaM3rWlkCNpNCVHPVcG2shZYdoB-bd1dTNxit8r4RnFzFfoD4KAP08m3H9__kMjzImhHGquwfPfsx3E_5Njn0SRnnwJswJSkYI7xueAbLDahiSCkau51BiVznINc5yFy5XOeQhcPXm23xzce_GrzWXppbXdrJudH8HzP47T2QPQHD3Z3M</recordid><startdate>19760501</startdate><enddate>19760501</enddate><creator>D H Hamer</creator><creator>C A Thomas, Jr</creator><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19760501</creationdate><title>Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI</title><author>D H Hamer ; C A Thomas, Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2837-ca29875fbbc1a56c13daaa92b5ff881d6492a0dd544cf0af5fb8826c75deffa93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Bacillus - enzymology</topic><topic>DNA</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA, Bacterial</topic><topic>Drosophila melanogaster</topic><topic>Endonucleases - metabolism</topic><topic>Escherichia coli</topic><topic>Extrachromosomal Inheritance</topic><topic>Genetic Engineering</topic><topic>Plasmids</topic><topic>Streptomyces - enzymology</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>D H Hamer</creatorcontrib><creatorcontrib>C A Thomas, Jr</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>D H Hamer</au><au>C A Thomas, Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1976-05-01</date><risdate>1976</risdate><volume>73</volume><issue>5</issue><spage>1537</spage><epage>1541</epage><pages>1537-1541</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be made with the tetracycline resistance factor pSC101, since insertion of DNA either at its single site for Sa1I or at that for BamI inactivates plasmid-specified drug resistance but not replication. To take advantage of this insertional inactivation, pSC101 was joined to a Co1E1-ampicillin resistance plasmid having no Sa1I site, and to a Co1E1-kanamycin resistance plasmid having no BamI site. Chimeras formed with the resulting hybrid vehicles can be identified simply by replica plating. These three vehicles, which all replicate under relaxed control, have been used to clone and amplify Drosophila melanogaster DNA fragments.</abstract><cop>United States</cop><pub>National Acad Sciences</pub><pmid>818638</pmid><doi>10.1073/pnas.73.5.1537</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1976-05, Vol.73 (5), p.1537-1541 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_818638 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bacillus - enzymology DNA DNA Restriction Enzymes - metabolism DNA, Bacterial Drosophila melanogaster Endonucleases - metabolism Escherichia coli Extrachromosomal Inheritance Genetic Engineering Plasmids Streptomyces - enzymology Transformation, Genetic |
| title | Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T19%3A11%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cloning%20of%20DNA%20fragments%20produced%20by%20restriction%20endonucleases%20Sa1I%20and%20BamI&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=D%20H%20Hamer&rft.date=1976-05-01&rft.volume=73&rft.issue=5&rft.spage=1537&rft.epage=1541&rft.pages=1537-1541&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.73.5.1537&rft_dat=%3Cproquest_pubme%3E83305475%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83305475&rft_id=info:pmid/818638&rfr_iscdi=true |