A direct ligand-binding radioassay for the measurement of methotrexate in tissues and biological fluids

A direct ligand-banding radioassay for methotrexate (MTX) has been developed using dihydrofolate reductase, contained in the lysate of L1210 leukemia cells, as the binding determinant. The procedure is a two-phase reaction system where standard MTX concentrations or the sample being assayed in incub...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cancer research (Chicago, Ill.) Ill.), 1975-08, Vol.35 (8), p.2033
Hauptverfasser:	Arons, E, Rothenberg, S P, Costa, M D, Fischer, C, Iqbal, M P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Binding Sites Dose-Response Relationship, Drug Folic Acid - pharmacology Humans Leukemia L1210 - analysis Mercaptoethanol - pharmacology Methotrexate - analysis Methotrexate - blood Methotrexate - metabolism Mice Mice, Inbred C57BL Mice, Inbred DBA NADPH-Ferrihemoprotein Reductase - pharmacology Radioligand Assay Stimulation, Chemical Tetrahydrofolate Dehydrogenase - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!