Improved Expression of a Hybrid Streptomyces clavuligerus cefE Gene in Penicillium chrysogenum

A hybrid cefE gene, encoding penicillin N expandase, was constructed by fusing the promoter sequences, Pcp, and terminator sequences, Pct from the Penicillium chrysogenum pcbC gene to the open reading frame (orf), cefEorf, from the Streptomyces clavuligerus cefE gene. The resulting hybrid gene, Pcp/...

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Veröffentlicht in:	Annals of the New York Academy of Sciences 1994-06, Vol.721 (1), p.178-193
Hauptverfasser:	QUEENER, STEPHEN W., BECKMANN, ROBERT J., CANTWELL, CATHLEEN A., HODGES, ROLAND L., FISHER, DEBORAH L., DOTZLAF, JOE E., YEH, WU-KUANG, McGILVRAY, DEREK, GREANEY, MICHAEL, ROSTECK, PAUL
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cephalosporins - biosynthesis Cephalosporins - chemistry DNA, Recombinant - genetics Gene Expression Genes, Bacterial Genetic Engineering Genetic Vectors Intramolecular Transferases Isomerases - genetics Molecular Sequence Data Mutagenesis Penicillin-Binding Proteins Penicillium - genetics Plasmids - genetics Streptomyces - enzymology Streptomyces - genetics Transformation, Genetic
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