Hexamethyldisilazane as a drying agent for pollen scanning electron microscopy
Use of hexamethyldisilazane (HMDS) as a final dehydrating solution provides robust, undistorted secondary electron images of a variety of angiosperm and gymnosperm pollen grains, including those considered to be susceptible to collapse in the scanning electron microscope. Ease of handling, low cost,...
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| Veröffentlicht in: | Biotechnic & histochemistry 1994-07, Vol.69 (4), p.192-198 |
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| container_end_page | 198 |
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| container_issue | 4 |
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| container_title | Biotechnic & histochemistry |
| container_volume | 69 |
| creator | Chissoe, W.F Vezey, E.L Skvarla, J.J |
| description | Use of hexamethyldisilazane (HMDS) as a final dehydrating solution provides robust, undistorted secondary electron images of a variety of angiosperm and gymnosperm pollen grains, including those considered to be susceptible to collapse in the scanning electron microscope. Ease of handling, low cost, lack of specialized equipment, minimal expenditure of time, and high rate of success are factors that favor HMDS over other drying agents for preparing pollen grains for scanning' electron microscopy. |
| doi_str_mv | 10.3109/10520299409106286 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1052-0295 |
| ispartof | Biotechnic & histochemistry, 1994-07, Vol.69 (4), p.192-198 |
| issn | 1052-0295 1473-7760 |
| language | eng |
| recordid | cdi_pubmed_primary_7918833 |
| source | MEDLINE; Taylor & Francis Journals Complete |
| subjects | ANGIOSPERMAS ANGIOSPERME ANGIOSPERMS Desiccation DRYING ECHANTILLONNAGE GIMNOSPERMAS GYMNOSPERME GYMNOSPERMS hexamethyldisilazane HMDS MICROSCOPIA MICROSCOPIE MICROSCOPY Microscopy, Electron, Scanning MUESTREO Organosilicon Compounds POLEN POLLEN Pollen - ultrastructure SAMPLE PRETREATMENT SAMPLING SECADO SECHAGE SEM SOLVANT SOLVENTES SOLVENTS |
| title | Hexamethyldisilazane as a drying agent for pollen scanning electron microscopy |
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