A variant glucocorticoid receptor messenger RNA is expressed in multiple myeloma patients
In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877-3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1995-07, Vol.55 (13), p.2727-2729 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | KRETT, N. L PILLAY, S MOALLI, P. A GREIPP, P. R ROSEN, S. T |
| description | In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877-3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present in myeloma patients. We detected both the wild-type and variant GR transcripts in the patient isolate that was the source of our myeloma cell lines, in patients refractory to steroid treatment, and in healthy control subjects. Simultaneous amplification of wild-type and variant GR mRNAs indicates that the variant GR is more highly expressed in cells that are resistant to glucocorticoids. We hypothesize that the variant GR is a normal mRNA transcript that acts to modulate glucocorticoid responsiveness, and increased expression contributes to a resistant phenotype. |
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L ; PILLAY, S ; MOALLI, P. A ; GREIPP, P. R ; ROSEN, S. T</creator><creatorcontrib>KRETT, N. L ; PILLAY, S ; MOALLI, P. A ; GREIPP, P. R ; ROSEN, S. T</creatorcontrib><description>In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877-3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present in myeloma patients. We detected both the wild-type and variant GR transcripts in the patient isolate that was the source of our myeloma cell lines, in patients refractory to steroid treatment, and in healthy control subjects. Simultaneous amplification of wild-type and variant GR mRNAs indicates that the variant GR is more highly expressed in cells that are resistant to glucocorticoids. We hypothesize that the variant GR is a normal mRNA transcript that acts to modulate glucocorticoid responsiveness, and increased expression contributes to a resistant phenotype.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 7796394</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA Primers - chemistry ; Gene Expression Regulation, Neoplastic ; Humans ; Immunodeficiencies. Immunoglobulinopathies ; Immunoglobulinopathies ; Immunopathology ; Medical sciences ; Molecular Sequence Data ; Multiple Myeloma - genetics ; Polymerase Chain Reaction ; Receptors, Glucocorticoid - genetics ; RNA, Messenger - genetics</subject><ispartof>Cancer research (Chicago, Ill.), 1995-07, Vol.55 (13), p.2727-2729</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3585046$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7796394$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KRETT, N. L</creatorcontrib><creatorcontrib>PILLAY, S</creatorcontrib><creatorcontrib>MOALLI, P. A</creatorcontrib><creatorcontrib>GREIPP, P. R</creatorcontrib><creatorcontrib>ROSEN, S. T</creatorcontrib><title>A variant glucocorticoid receptor messenger RNA is expressed in multiple myeloma patients</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877-3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present in myeloma patients. We detected both the wild-type and variant GR transcripts in the patient isolate that was the source of our myeloma cell lines, in patients refractory to steroid treatment, and in healthy control subjects. Simultaneous amplification of wild-type and variant GR mRNAs indicates that the variant GR is more highly expressed in cells that are resistant to glucocorticoids. We hypothesize that the variant GR is a normal mRNA transcript that acts to modulate glucocorticoid responsiveness, and increased expression contributes to a resistant phenotype.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Primers - chemistry</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Multiple Myeloma - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>RNA, Messenger - genetics</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9T8tKxDAADKKsdfUThBy8FvJs0mNZfMGiIHrwtKR5rJG0DUkq7t-7YpE5DPNgYE5AhTmVtWCMn4IKISRrzgQ5Bxc5fx4lx4ivwEqItqEtq8B7B79U8moscB9mPekpFa8nb2Cy2sYyJTjYnO24twm-PHXQZ2i_Y_r1DPQjHOZQfAwWDgcbpkHBqIq3Y8mX4MypkO3Vwmvwdnf7unmot8_3j5tuW38QKkstTeNEw4ThLRFSS6eF7HvUMkuaI7DizmFqMBUEM0aoQw4TI5BASDWyxXQNrv9249wP1uxi8oNKh91y8ZjfLLnKWgWX1Kh9_q9RLjliDf0BRGhayQ</recordid><startdate>19950701</startdate><enddate>19950701</enddate><creator>KRETT, N. 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L</creatorcontrib><creatorcontrib>PILLAY, S</creatorcontrib><creatorcontrib>MOALLI, P. A</creatorcontrib><creatorcontrib>GREIPP, P. R</creatorcontrib><creatorcontrib>ROSEN, S. T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KRETT, N. L</au><au>PILLAY, S</au><au>MOALLI, P. A</au><au>GREIPP, P. R</au><au>ROSEN, S. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A variant glucocorticoid receptor messenger RNA is expressed in multiple myeloma patients</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1995-07-01</date><risdate>1995</risdate><volume>55</volume><issue>13</issue><spage>2727</spage><epage>2729</epage><pages>2727-2729</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>In multiple myeloma cells resistant to glucocorticoids, we have previously identified a variant glucocorticoid receptor (GR) transcript (P. A. Moalli et al., Cancer Res., 53: 3877-3879, 1993). Here, we report a reverse transcription-PCR assay to assess whether this aberrant GR transcript is present in myeloma patients. We detected both the wild-type and variant GR transcripts in the patient isolate that was the source of our myeloma cell lines, in patients refractory to steroid treatment, and in healthy control subjects. Simultaneous amplification of wild-type and variant GR mRNAs indicates that the variant GR is more highly expressed in cells that are resistant to glucocorticoids. We hypothesize that the variant GR is a normal mRNA transcript that acts to modulate glucocorticoid responsiveness, and increased expression contributes to a resistant phenotype.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>7796394</pmid><tpages>3</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1995-07, Vol.55 (13), p.2727-2729 |
| issn | 0008-5472 1538-7445 |
| language | eng |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Base Sequence Biological and medical sciences DNA Primers - chemistry Gene Expression Regulation, Neoplastic Humans Immunodeficiencies. Immunoglobulinopathies Immunoglobulinopathies Immunopathology Medical sciences Molecular Sequence Data Multiple Myeloma - genetics Polymerase Chain Reaction Receptors, Glucocorticoid - genetics RNA, Messenger - genetics |
| title | A variant glucocorticoid receptor messenger RNA is expressed in multiple myeloma patients |
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