Bovine Pancreatic Trypsin Inhibitor-Trypsin Complex as a Detection System for Recombinant Proteins

Bovine pancreatic trypsin inhibitor (BPTI) binds to trypsin and anhydrotrypsin (an enzymatically inactive derivative of trypsin) with affinities of 6 x 10-14and 1.1 x 10-13M, respectively. We have taken advantage of the high affinity and specificity of this binding reaction to develop a protein tagg...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1993-01, Vol.90 (1), p.337-341
Hauptverfasser:	Borjigin, Jimo, Nathans, Jeremy
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aprotinin - chemistry Aprotinin - genetics Aprotinin - metabolism Biochemistry Biological and medical sciences Cattle Cell membranes complex Detection systems Diverse techniques Fundamental and applied biological sciences. Psychology Gels growth hormone Hormones Humans Indicators and Reagents Laboratory staining techniques Molecular and cellular biology mutagenesis opsin Opsins pancreatic trypsin inhibitor Protein Structure, Secondary protein tagging Proteins Rabbits Receptors Receptors, Somatotropin - analysis Receptors, Somatotropin - genetics Receptors, Somatotropin - metabolism Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - metabolism Recombinant proteins Rod Opsins - analysis Rod Opsins - genetics Rod Opsins - metabolism Spectrophotometry Transfection trypsin Trypsin - chemistry Trypsin - metabolism Ungulates
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