[20] Assay for thiols based on reactivation of papain

This chapter describes a sensitive spectrophotometric assay for the quantitation of thiol groups that is based on reactivation of papain. The assay involves the reaction of a thiol with an inactive mixed disulfide of papain, resulting in the stoichiometric formation of active papain. The activated p...

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Veröffentlicht in:	Methods in Enzymology 1995, Vol.251, p.229-237
Hauptverfasser:	Singh, Rajeeva, Blättler, Walter A., Collinson, Albert R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals assays Binding Sites chemical reactions Cysteine - pharmacology Disulfides Dithionitrobenzoic Acid - pharmacology Edetic Acid Fabaceae - enzymology Fructose-Bisphosphate Aldolase - chemistry Indicators and Reagents Kinetics Methyl Methanesulfonate - analogs & derivatives Methyl Methanesulfonate - pharmacology Muscle, Skeletal - enzymology Oxidation-Reduction papain Papain - metabolism Plants, Medicinal Proteins - chemistry quantitative analysis Rabbits Sensitivity and Specificity Serum Albumin, Bovine - chemistry Spectrophotometry - methods spectroscopy Sulfhydryl Compounds - analysis Sulfhydryl Compounds - pharmacology thiols Urease - chemistry
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