Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere
The genetically engineered transposon TnPCB, contains genes (bph) encoding the biphenyl degradative pathway. TnPCB was stably inserted into the chromosome of two different rhizosphere pseudomonads. One genetically modified strain, Pseudomonas fluorescens F113pcb, was characterized in detail and foun...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1995-05, Vol.61 (5), p.1946-1952 |
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| container_title | Applied and Environmental Microbiology |
| container_volume | 61 |
| creator | Brazil, G M Kenefick, L Callanan, M Haro, A de Lorenzo, V Dowling, D N O'Gara, F |
| description | The genetically engineered transposon TnPCB, contains genes (bph) encoding the biphenyl degradative pathway. TnPCB was stably inserted into the chromosome of two different rhizosphere pseudomonads. One genetically modified strain, Pseudomonas fluorescens F113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. The expression of the heterologous bph genes in F113pcb was confirmed by the ability of the genetically modified microorganism to utilize biphenyl as a sole carbon source. The introduced trait remained stable in laboratory experiments, and no bph-negative isolates were found after extensive subculture in nonselective media. The bph trait was also stable in nonselective rhizosphere microcosms. Rhizosphere competence of the modified F113pcb was assessed in colonization experiments in nonsterile soil microcosms on sugar beet seedling roots. F113pcb was able to colonize as efficiently as a marked wild-type strain, and no decrease in competitiveness was observed. In situ expression of the bph genes in F113pcb was found when F113pcb bearing a bph'lacZ reporter fusion was inoculated onto sugar beet seeds. This indicates that the bph gene products may also be present under in situ conditions. These experiments demonstrated that rhizosphere-adapted microbes can be genetically manipulated to metabolize novel compounds without affecting their ecological competence. Expression of the introduced genes can be detected in the rhizosphere, indicating considerable potential for the manipulation of the rhizosphere as a self-sustaining biofilm for the bioremediation of pollutants in soil. Rhizosphere bacteria such as fluorescent Pseudomonas spp. are ecologically adapted to colonize and compete in the rhizosphere environment. Expanding the metabolic functions of such pseudomonads to degrade pollutants may prove to be a useful strategy for bioremediation. |
| doi_str_mv | 10.1128/AEM.61.5.1946-1952.1995 |
| format | Article |
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Mediterranean Action Plan. FAO, Rome (Italy). IAEA, Vienna (Austria)</creatorcontrib><description>The genetically engineered transposon TnPCB, contains genes (bph) encoding the biphenyl degradative pathway. TnPCB was stably inserted into the chromosome of two different rhizosphere pseudomonads. One genetically modified strain, Pseudomonas fluorescens F113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. The expression of the heterologous bph genes in F113pcb was confirmed by the ability of the genetically modified microorganism to utilize biphenyl as a sole carbon source. The introduced trait remained stable in laboratory experiments, and no bph-negative isolates were found after extensive subculture in nonselective media. The bph trait was also stable in nonselective rhizosphere microcosms. Rhizosphere competence of the modified F113pcb was assessed in colonization experiments in nonsterile soil microcosms on sugar beet seedling roots. F113pcb was able to colonize as efficiently as a marked wild-type strain, and no decrease in competitiveness was observed. In situ expression of the bph genes in F113pcb was found when F113pcb bearing a bph'lacZ reporter fusion was inoculated onto sugar beet seeds. This indicates that the bph gene products may also be present under in situ conditions. These experiments demonstrated that rhizosphere-adapted microbes can be genetically manipulated to metabolize novel compounds without affecting their ecological competence. Expression of the introduced genes can be detected in the rhizosphere, indicating considerable potential for the manipulation of the rhizosphere as a self-sustaining biofilm for the bioremediation of pollutants in soil. Rhizosphere bacteria such as fluorescent Pseudomonas spp. are ecologically adapted to colonize and compete in the rhizosphere environment. Expanding the metabolic functions of such pseudomonads to degrade pollutants may prove to be a useful strategy for bioremediation.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.61.5.1946-1952.1995</identifier><identifier>PMID: 7646029</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; beta vulgaris ; bifenilos policlorados ; biodegradacion ; biodegradation ; Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Biology ; Biotechnology ; Cloning, Molecular ; control de la contaminacion ; DNA Transposable Elements - genetics ; Environment and pollution ; expresion genica ; expression des genes ; Fundamental and applied biological sciences. Psychology ; gene ; gene expression ; Gene Expression Regulation, Bacterial ; gene transfer ; Genes ; Genetic Engineering ; genetic transformation ; Genetics ; Industrial applications and implications. Economical aspects ; inoculacion ; inoculation ; inoculation methods ; lutte antipollution ; Multienzyme Complexes - biosynthesis ; Multienzyme Complexes - genetics ; Operon ; Oxidoreductases - biosynthesis ; Oxidoreductases - genetics ; Oxygenases - biosynthesis ; Oxygenases - genetics ; pollution control ; polychlorinated biphenyls ; Polychlorinated Biphenyls - metabolism ; polychlorure de biphenyle ; Pseudomonas fluorescens ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - growth & development ; Pseudomonas fluorescens - metabolism ; Recombinant Fusion Proteins - biosynthesis ; rhizosphere ; rizosfera ; seed ; semence ; semillas ; Soil Microbiology ; Soil Pollutants - metabolism ; transferencia de genes ; transfert de gene ; transformacion genetica ; transformation genetique ; Vegetables - microbiology</subject><ispartof>Applied and Environmental Microbiology, 1995-05, Vol.61 (5), p.1946-1952</ispartof><rights>1995 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c583t-2625da3640b9f71f32f5e85bde6b685560aecc6b5af14f09eac8f33bad21ff7e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC167456/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC167456/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3512394$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7646029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brazil, G M</creatorcontrib><creatorcontrib>Kenefick, L</creatorcontrib><creatorcontrib>Callanan, M</creatorcontrib><creatorcontrib>Haro, A</creatorcontrib><creatorcontrib>de Lorenzo, V</creatorcontrib><creatorcontrib>Dowling, D N</creatorcontrib><creatorcontrib>O'Gara, F</creatorcontrib><creatorcontrib>University College, Cork, Ireland</creatorcontrib><creatorcontrib>UNEP, Athens (Greece). Mediterranean Action Plan. FAO, Rome (Italy). IAEA, Vienna (Austria)</creatorcontrib><title>Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The genetically engineered transposon TnPCB, contains genes (bph) encoding the biphenyl degradative pathway. TnPCB was stably inserted into the chromosome of two different rhizosphere pseudomonads. One genetically modified strain, Pseudomonas fluorescens F113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. The expression of the heterologous bph genes in F113pcb was confirmed by the ability of the genetically modified microorganism to utilize biphenyl as a sole carbon source. The introduced trait remained stable in laboratory experiments, and no bph-negative isolates were found after extensive subculture in nonselective media. The bph trait was also stable in nonselective rhizosphere microcosms. Rhizosphere competence of the modified F113pcb was assessed in colonization experiments in nonsterile soil microcosms on sugar beet seedling roots. F113pcb was able to colonize as efficiently as a marked wild-type strain, and no decrease in competitiveness was observed. In situ expression of the bph genes in F113pcb was found when F113pcb bearing a bph'lacZ reporter fusion was inoculated onto sugar beet seeds. This indicates that the bph gene products may also be present under in situ conditions. These experiments demonstrated that rhizosphere-adapted microbes can be genetically manipulated to metabolize novel compounds without affecting their ecological competence. Expression of the introduced genes can be detected in the rhizosphere, indicating considerable potential for the manipulation of the rhizosphere as a self-sustaining biofilm for the bioremediation of pollutants in soil. Rhizosphere bacteria such as fluorescent Pseudomonas spp. are ecologically adapted to colonize and compete in the rhizosphere environment. Expanding the metabolic functions of such pseudomonads to degrade pollutants may prove to be a useful strategy for bioremediation.</description><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>beta vulgaris</subject><subject>bifenilos policlorados</subject><subject>biodegradacion</subject><subject>biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biology</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>control de la contaminacion</subject><subject>DNA Transposable Elements - genetics</subject><subject>Environment and pollution</subject><subject>expresion genica</subject><subject>expression des genes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>gene transfer</subject><subject>Genes</subject><subject>Genetic Engineering</subject><subject>genetic transformation</subject><subject>Genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>inoculacion</subject><subject>inoculation</subject><subject>inoculation methods</subject><subject>lutte antipollution</subject><subject>Multienzyme Complexes - biosynthesis</subject><subject>Multienzyme Complexes - genetics</subject><subject>Operon</subject><subject>Oxidoreductases - biosynthesis</subject><subject>Oxidoreductases - genetics</subject><subject>Oxygenases - biosynthesis</subject><subject>Oxygenases - genetics</subject><subject>pollution control</subject><subject>polychlorinated biphenyls</subject><subject>Polychlorinated Biphenyls - metabolism</subject><subject>polychlorure de biphenyle</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - growth & development</subject><subject>Pseudomonas fluorescens - metabolism</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>rhizosphere</subject><subject>rizosfera</subject><subject>seed</subject><subject>semence</subject><subject>semillas</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - metabolism</subject><subject>transferencia de genes</subject><subject>transfert de gene</subject><subject>transformacion genetica</subject><subject>transformation genetique</subject><subject>Vegetables - microbiology</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks9u1DAQxiMEKkvhEQCDELcsdhx740MP1ar8kYo4QM_WJBlvXCV2sBPK8h68L452tWo52fL8vm9m9DnLXjO6ZqyoPlxefV1LthZrpkqZMyWKdFPiUbZiVFW54Fw-zlaUKpUXRUmfZs9ivKWUllRWZ9nZRpaSFmqV_d16F6cwN5P1jnhDgITO_vFx7DAgGSPOrR-8g5bc2akjo5_QTRZ6MnnS4i5Amyjf75uu98E6mLAltU1qt-8jAdcmasKTfT12ZIcOCf4eA8a4PFtHpg7v932ePTHQR3xxPM-zm49XP7af8-tvn75sL6_zRlR8ygtZiBa4LGmtzIYZXhiBlahblLWshJAUsGlkLcCw0lCF0FSG8xraghmzQX6eXRx8x7kesG3SagF6PQY7QNhrD1Y_rDjb6Z3_pZnclEIm_fujPvifM8ZJDzY22Pfg0M9xwaSinCXw7X_grZ-DS7vpggrFWUUXt80BaoKPMaA5DcKoXlLXgIOWTAu9pK6X1PWSelK-vL_HSXeMOdXfHesQG-hNANfYeMK4YAVXZcLeHLDO7ro7G1BDHB42TcyrA2PAa9iFZHPzPQ0hKU3_TJT8H-b2z_I</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>Brazil, G M</creator><creator>Kenefick, L</creator><creator>Callanan, M</creator><creator>Haro, A</creator><creator>de Lorenzo, V</creator><creator>Dowling, D N</creator><creator>O'Gara, F</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7TV</scope><scope>5PM</scope></search><sort><creationdate>19950501</creationdate><title>Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere</title><author>Brazil, G M ; Kenefick, L ; Callanan, M ; Haro, A ; de Lorenzo, V ; Dowling, D N ; O'Gara, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c583t-2625da3640b9f71f32f5e85bde6b685560aecc6b5af14f09eac8f33bad21ff7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>beta vulgaris</topic><topic>bifenilos policlorados</topic><topic>biodegradacion</topic><topic>biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biology</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>control de la contaminacion</topic><topic>DNA Transposable Elements - genetics</topic><topic>Environment and pollution</topic><topic>expresion genica</topic><topic>expression des genes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>gene transfer</topic><topic>Genes</topic><topic>Genetic Engineering</topic><topic>genetic transformation</topic><topic>Genetics</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>inoculacion</topic><topic>inoculation</topic><topic>inoculation methods</topic><topic>lutte antipollution</topic><topic>Multienzyme Complexes - biosynthesis</topic><topic>Multienzyme Complexes - genetics</topic><topic>Operon</topic><topic>Oxidoreductases - biosynthesis</topic><topic>Oxidoreductases - genetics</topic><topic>Oxygenases - biosynthesis</topic><topic>Oxygenases - genetics</topic><topic>pollution control</topic><topic>polychlorinated biphenyls</topic><topic>Polychlorinated Biphenyls - metabolism</topic><topic>polychlorure de biphenyle</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - growth & development</topic><topic>Pseudomonas fluorescens - metabolism</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>rhizosphere</topic><topic>rizosfera</topic><topic>seed</topic><topic>semence</topic><topic>semillas</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - metabolism</topic><topic>transferencia de genes</topic><topic>transfert de gene</topic><topic>transformacion genetica</topic><topic>transformation genetique</topic><topic>Vegetables - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brazil, G M</creatorcontrib><creatorcontrib>Kenefick, L</creatorcontrib><creatorcontrib>Callanan, M</creatorcontrib><creatorcontrib>Haro, A</creatorcontrib><creatorcontrib>de Lorenzo, V</creatorcontrib><creatorcontrib>Dowling, D N</creatorcontrib><creatorcontrib>O'Gara, F</creatorcontrib><creatorcontrib>University College, Cork, Ireland</creatorcontrib><creatorcontrib>UNEP, Athens (Greece). Mediterranean Action Plan. FAO, Rome (Italy). IAEA, Vienna (Austria)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brazil, G M</au><au>Kenefick, L</au><au>Callanan, M</au><au>Haro, A</au><au>de Lorenzo, V</au><au>Dowling, D N</au><au>O'Gara, F</au><aucorp>University College, Cork, Ireland</aucorp><aucorp>UNEP, Athens (Greece). Mediterranean Action Plan. FAO, Rome (Italy). IAEA, Vienna (Austria)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>61</volume><issue>5</issue><spage>1946</spage><epage>1952</epage><pages>1946-1952</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The genetically engineered transposon TnPCB, contains genes (bph) encoding the biphenyl degradative pathway. TnPCB was stably inserted into the chromosome of two different rhizosphere pseudomonads. One genetically modified strain, Pseudomonas fluorescens F113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. The expression of the heterologous bph genes in F113pcb was confirmed by the ability of the genetically modified microorganism to utilize biphenyl as a sole carbon source. The introduced trait remained stable in laboratory experiments, and no bph-negative isolates were found after extensive subculture in nonselective media. The bph trait was also stable in nonselective rhizosphere microcosms. Rhizosphere competence of the modified F113pcb was assessed in colonization experiments in nonsterile soil microcosms on sugar beet seedling roots. F113pcb was able to colonize as efficiently as a marked wild-type strain, and no decrease in competitiveness was observed. In situ expression of the bph genes in F113pcb was found when F113pcb bearing a bph'lacZ reporter fusion was inoculated onto sugar beet seeds. This indicates that the bph gene products may also be present under in situ conditions. These experiments demonstrated that rhizosphere-adapted microbes can be genetically manipulated to metabolize novel compounds without affecting their ecological competence. Expression of the introduced genes can be detected in the rhizosphere, indicating considerable potential for the manipulation of the rhizosphere as a self-sustaining biofilm for the bioremediation of pollutants in soil. Rhizosphere bacteria such as fluorescent Pseudomonas spp. are ecologically adapted to colonize and compete in the rhizosphere environment. Expanding the metabolic functions of such pseudomonads to degrade pollutants may prove to be a useful strategy for bioremediation.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>7646029</pmid><doi>10.1128/AEM.61.5.1946-1952.1995</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 1995-05, Vol.61 (5), p.1946-1952 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmed_primary_7646029 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - genetics beta vulgaris bifenilos policlorados biodegradacion biodegradation Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Biology Biotechnology Cloning, Molecular control de la contaminacion DNA Transposable Elements - genetics Environment and pollution expresion genica expression des genes Fundamental and applied biological sciences. Psychology gene gene expression Gene Expression Regulation, Bacterial gene transfer Genes Genetic Engineering genetic transformation Genetics Industrial applications and implications. Economical aspects inoculacion inoculation inoculation methods lutte antipollution Multienzyme Complexes - biosynthesis Multienzyme Complexes - genetics Operon Oxidoreductases - biosynthesis Oxidoreductases - genetics Oxygenases - biosynthesis Oxygenases - genetics pollution control polychlorinated biphenyls Polychlorinated Biphenyls - metabolism polychlorure de biphenyle Pseudomonas fluorescens Pseudomonas fluorescens - genetics Pseudomonas fluorescens - growth & development Pseudomonas fluorescens - metabolism Recombinant Fusion Proteins - biosynthesis rhizosphere rizosfera seed semence semillas Soil Microbiology Soil Pollutants - metabolism transferencia de genes transfert de gene transformacion genetica transformation genetique Vegetables - microbiology |
| title | Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere |
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