SV40-immortalized and primary cultured human retinal pigment epithelial cells share similar patterns of cytokine-receptor expression and cytokine responsiveness

Retinal pigment epithelial (RPE) cells produce and respond to a variety of cytokines; however, molecular and biochemical studies are restricted by the limited access to large numbers of pure cells and the variability associated with different donor sources. Despite success in establishing primary hu...

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Veröffentlicht in:	Current eye research 1995, Vol.14 (6), p.495-503
Hauptverfasser:	Sippy, Brian D., Hofman, Florence M., He, Shikun, Osusky, Roman, Sheu, Shwu-Jiuan, Walker, Sharyn M., Ryan, Stephen J., Hinton, David R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence cell culture Cell Division Cell Line, Transformed Cell Transformation, Viral - physiology Cells, Cultured cytokines Cytokines - biosynthesis Cytokines - pharmacology DNA Probes - chemistry Flow Cytometry human Humans intercellular adhesion molecule (ICAM)-1 Molecular Sequence Data Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - drug effects Pigment Epithelium of Eye - metabolism Polymerase Chain Reaction Receptors, Cytokine - biosynthesis retinal pigment epithelium (RPE) reverse transcriptase-polymerase chain reaction (RT-PCR) RNA, Messenger - metabolism RNA-Directed DNA Polymerase simian virus (SV)-40 Simian virus 40 - physiology tumor necrosis factor-α (TNF-α)
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