Preparation of Radioactive Labelled (57Co) Sulfitocobalamin
Farquharson and Adams (Br. J. Nutr. 36, 127-135 (1976)) have identified sulfitocobalamin (S0 3 −Cbl) as one of the naturally occurring cobalamins (Cbls) in foods. We have devised a method of making radioactive labelled S0 3 −Cbl for invivo and in vitro studies of this form of Cbl. 57 Co labelled cya...
Gespeichert in:
| Veröffentlicht in: | Prep. Biochem.; (United States) 1978-01, Vol.8 (6), p.411-419 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 419 |
|---|---|
| container_issue | 6 |
| container_start_page | 411 |
| container_title | Prep. Biochem.; (United States) |
| container_volume | 8 |
| creator | Begley, James A. Horch, Carol Hall, Charles A. |
| description | Farquharson and Adams (Br. J. Nutr. 36, 127-135 (1976)) have identified sulfitocobalamin (S0
3
−Cbl) as one of the naturally occurring cobalamins (Cbls) in foods. We have devised a method of making radioactive labelled S0
3
−Cbl for invivo and in vitro studies of this form of Cbl.
57
Co labelled cyanocobalamin (
57
Co CN-Cbl) was acid photolyzed to
57
Co hydroxocobalamin (
57
Co OH-Cbl) followed by ligand substitution with S0
3
−2
ion from aqueous sodium (meta) bisulfite in the dark. The resulting
57
Co SO
3
−Cbl was purified by organic extraction and cation ex-change chromatography. The final preparation was >99% Co
57
S0
3
−Cbl with an overall yield of >70%, stable for up to four weeks at 20°C in the dark, and capable of binding to the human Cbl binding proteins Transcobalamin II (TC II), Intrinsic factor (IF) and Salivary R. This method allows a simple 1 day preparation of high specific activity labelled
57
Co S0
3
−Cbl for biological studies. |
| doi_str_mv | 10.1080/00327487808061659 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_748915</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>74375708</sourcerecordid><originalsourceid>FETCH-LOGICAL-c290t-75ecc146b2dcdbb301baaed2687410c2591e8e888e85ced007da0ebbdeae4b4b3</originalsourceid><addsrcrecordid>eNp1UEtLxDAQzsH34wcIHooH0cNq0jZNFr3I4gsWFB_nMEmmGGmbNckq_nsjFS_iYRjmezF8hOwxesKopKeUVqWopZD5aFjDpytk8xubZLDeIFsxvuaT8apcJ2sZmzK-Sc7uAy4gQHJ-KHxbPIB1Hkxy71jMQWPXoS2OuJj54-Jx2bUueeM1dNC7YYesttBF3P3Z2-T56vJpdjOZ313fzi7mE1NOaZoIjsawutGlNVbrijINgLZspKgZNSWfMpQoZR5u0FIqLFDU2iJgrWtdbZODMdfH5FQ0LqF5MX4Y0CTVyLKkFc-iw1G0CP5tiTGp3kWT_4cB_TIqUVeCCyqzkI1CE3yMAVu1CK6H8KkYVd9Fqj9FZs_-T_hS92h_HWOLmT4faTe0PvTw4UNnVYLPzoc2wGBcVNX_6V8vFYHR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74375708</pqid></control><display><type>article</type><title>Preparation of Radioactive Labelled (57Co) Sulfitocobalamin</title><source>MEDLINE</source><source>Taylor & Francis:Master (3349 titles)</source><creator>Begley, James A. ; Horch, Carol ; Hall, Charles A.</creator><creatorcontrib>Begley, James A. ; Horch, Carol ; Hall, Charles A. ; Veterans Administration Hosp., Albany, NY</creatorcontrib><description>Farquharson and Adams (Br. J. Nutr. 36, 127-135 (1976)) have identified sulfitocobalamin (S0
3
−Cbl) as one of the naturally occurring cobalamins (Cbls) in foods. We have devised a method of making radioactive labelled S0
3
−Cbl for invivo and in vitro studies of this form of Cbl.
57
Co labelled cyanocobalamin (
57
Co CN-Cbl) was acid photolyzed to
57
Co hydroxocobalamin (
57
Co OH-Cbl) followed by ligand substitution with S0
3
−2
ion from aqueous sodium (meta) bisulfite in the dark. The resulting
57
Co SO
3
−Cbl was purified by organic extraction and cation ex-change chromatography. The final preparation was >99% Co
57
S0
3
−Cbl with an overall yield of >70%, stable for up to four weeks at 20°C in the dark, and capable of binding to the human Cbl binding proteins Transcobalamin II (TC II), Intrinsic factor (IF) and Salivary R. This method allows a simple 1 day preparation of high specific activity labelled
57
Co S0
3
−Cbl for biological studies.</description><identifier>ISSN: 0032-7484</identifier><identifier>DOI: 10.1080/00327487808061659</identifier><identifier>PMID: 748915</identifier><language>eng</language><publisher>United States: Taylor & Francis Group</publisher><subject>550601 -- Medicine-- Unsealed Radionuclides in Diagnostics ; BETA DECAY RADIOISOTOPES ; CHEMICAL PREPARATION ; CHEMISTRY ; COBALT 57 ; COBALT ISOTOPES ; Cobalt Radioisotopes ; DAYS LIVING RADIOISOTOPES ; ELECTRON CAPTURE RADIOISOTOPES ; INTERMEDIATE MASS NUCLEI ; Isotope Labeling - methods ; ISOTOPES ; LABELLED COMPOUNDS ; LABELLING ; MEDICINE ; Methods ; NUCLEAR MEDICINE ; NUCLEI ; ODD-EVEN NUCLEI ; RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY ; RADIOCHEMISTRY ; RADIOISOTOPES ; RADIOLOGY AND NUCLEAR MEDICINE ; SYNTHESIS ; Vitamin B 12 - chemical synthesis ; VITAMIN B GROUP ; VITAMIN B-12 ; VITAMINS 400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials</subject><ispartof>Prep. Biochem.; (United States), 1978-01, Vol.8 (6), p.411-419</ispartof><rights>Copyright Taylor & Francis Group, LLC 1978</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-75ecc146b2dcdbb301baaed2687410c2591e8e888e85ced007da0ebbdeae4b4b3</citedby><cites>FETCH-LOGICAL-c290t-75ecc146b2dcdbb301baaed2687410c2591e8e888e85ced007da0ebbdeae4b4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00327487808061659$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00327487808061659$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,885,4014,27914,27915,27916,59636,60425</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/748915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6822035$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Begley, James A.</creatorcontrib><creatorcontrib>Horch, Carol</creatorcontrib><creatorcontrib>Hall, Charles A.</creatorcontrib><creatorcontrib>Veterans Administration Hosp., Albany, NY</creatorcontrib><title>Preparation of Radioactive Labelled (57Co) Sulfitocobalamin</title><title>Prep. Biochem.; (United States)</title><addtitle>Prep Biochem</addtitle><description>Farquharson and Adams (Br. J. Nutr. 36, 127-135 (1976)) have identified sulfitocobalamin (S0
3
−Cbl) as one of the naturally occurring cobalamins (Cbls) in foods. We have devised a method of making radioactive labelled S0
3
−Cbl for invivo and in vitro studies of this form of Cbl.
57
Co labelled cyanocobalamin (
57
Co CN-Cbl) was acid photolyzed to
57
Co hydroxocobalamin (
57
Co OH-Cbl) followed by ligand substitution with S0
3
−2
ion from aqueous sodium (meta) bisulfite in the dark. The resulting
57
Co SO
3
−Cbl was purified by organic extraction and cation ex-change chromatography. The final preparation was >99% Co
57
S0
3
−Cbl with an overall yield of >70%, stable for up to four weeks at 20°C in the dark, and capable of binding to the human Cbl binding proteins Transcobalamin II (TC II), Intrinsic factor (IF) and Salivary R. This method allows a simple 1 day preparation of high specific activity labelled
57
Co S0
3
−Cbl for biological studies.</description><subject>550601 -- Medicine-- Unsealed Radionuclides in Diagnostics</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>CHEMICAL PREPARATION</subject><subject>CHEMISTRY</subject><subject>COBALT 57</subject><subject>COBALT ISOTOPES</subject><subject>Cobalt Radioisotopes</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>Isotope Labeling - methods</subject><subject>ISOTOPES</subject><subject>LABELLED COMPOUNDS</subject><subject>LABELLING</subject><subject>MEDICINE</subject><subject>Methods</subject><subject>NUCLEAR MEDICINE</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY</subject><subject>RADIOCHEMISTRY</subject><subject>RADIOISOTOPES</subject><subject>RADIOLOGY AND NUCLEAR MEDICINE</subject><subject>SYNTHESIS</subject><subject>Vitamin B 12 - chemical synthesis</subject><subject>VITAMIN B GROUP</subject><subject>VITAMIN B-12</subject><subject>VITAMINS 400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials</subject><issn>0032-7484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UEtLxDAQzsH34wcIHooH0cNq0jZNFr3I4gsWFB_nMEmmGGmbNckq_nsjFS_iYRjmezF8hOwxesKopKeUVqWopZD5aFjDpytk8xubZLDeIFsxvuaT8apcJ2sZmzK-Sc7uAy4gQHJ-KHxbPIB1Hkxy71jMQWPXoS2OuJj54-Jx2bUueeM1dNC7YYesttBF3P3Z2-T56vJpdjOZ313fzi7mE1NOaZoIjsawutGlNVbrijINgLZspKgZNSWfMpQoZR5u0FIqLFDU2iJgrWtdbZODMdfH5FQ0LqF5MX4Y0CTVyLKkFc-iw1G0CP5tiTGp3kWT_4cB_TIqUVeCCyqzkI1CE3yMAVu1CK6H8KkYVd9Fqj9FZs_-T_hS92h_HWOLmT4faTe0PvTw4UNnVYLPzoc2wGBcVNX_6V8vFYHR</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>Begley, James A.</creator><creator>Horch, Carol</creator><creator>Hall, Charles A.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19780101</creationdate><title>Preparation of Radioactive Labelled (57Co) Sulfitocobalamin</title><author>Begley, James A. ; Horch, Carol ; Hall, Charles A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-75ecc146b2dcdbb301baaed2687410c2591e8e888e85ced007da0ebbdeae4b4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>550601 -- Medicine-- Unsealed Radionuclides in Diagnostics</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>CHEMICAL PREPARATION</topic><topic>CHEMISTRY</topic><topic>COBALT 57</topic><topic>COBALT ISOTOPES</topic><topic>Cobalt Radioisotopes</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>Isotope Labeling - methods</topic><topic>ISOTOPES</topic><topic>LABELLED COMPOUNDS</topic><topic>LABELLING</topic><topic>MEDICINE</topic><topic>Methods</topic><topic>NUCLEAR MEDICINE</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY</topic><topic>RADIOCHEMISTRY</topic><topic>RADIOISOTOPES</topic><topic>RADIOLOGY AND NUCLEAR MEDICINE</topic><topic>SYNTHESIS</topic><topic>Vitamin B 12 - chemical synthesis</topic><topic>VITAMIN B GROUP</topic><topic>VITAMIN B-12</topic><topic>VITAMINS 400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials</topic><toplevel>online_resources</toplevel><creatorcontrib>Begley, James A.</creatorcontrib><creatorcontrib>Horch, Carol</creatorcontrib><creatorcontrib>Hall, Charles A.</creatorcontrib><creatorcontrib>Veterans Administration Hosp., Albany, NY</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Prep. Biochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Begley, James A.</au><au>Horch, Carol</au><au>Hall, Charles A.</au><aucorp>Veterans Administration Hosp., Albany, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of Radioactive Labelled (57Co) Sulfitocobalamin</atitle><jtitle>Prep. Biochem.; (United States)</jtitle><addtitle>Prep Biochem</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>8</volume><issue>6</issue><spage>411</spage><epage>419</epage><pages>411-419</pages><issn>0032-7484</issn><abstract>Farquharson and Adams (Br. J. Nutr. 36, 127-135 (1976)) have identified sulfitocobalamin (S0
3
−Cbl) as one of the naturally occurring cobalamins (Cbls) in foods. We have devised a method of making radioactive labelled S0
3
−Cbl for invivo and in vitro studies of this form of Cbl.
57
Co labelled cyanocobalamin (
57
Co CN-Cbl) was acid photolyzed to
57
Co hydroxocobalamin (
57
Co OH-Cbl) followed by ligand substitution with S0
3
−2
ion from aqueous sodium (meta) bisulfite in the dark. The resulting
57
Co SO
3
−Cbl was purified by organic extraction and cation ex-change chromatography. The final preparation was >99% Co
57
S0
3
−Cbl with an overall yield of >70%, stable for up to four weeks at 20°C in the dark, and capable of binding to the human Cbl binding proteins Transcobalamin II (TC II), Intrinsic factor (IF) and Salivary R. This method allows a simple 1 day preparation of high specific activity labelled
57
Co S0
3
−Cbl for biological studies.</abstract><cop>United States</cop><pub>Taylor & Francis Group</pub><pmid>748915</pmid><doi>10.1080/00327487808061659</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-7484 |
| ispartof | Prep. Biochem.; (United States), 1978-01, Vol.8 (6), p.411-419 |
| issn | 0032-7484 |
| language | eng |
| recordid | cdi_pubmed_primary_748915 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles) |
| subjects | 550601 -- Medicine-- Unsealed Radionuclides in Diagnostics BETA DECAY RADIOISOTOPES CHEMICAL PREPARATION CHEMISTRY COBALT 57 COBALT ISOTOPES Cobalt Radioisotopes DAYS LIVING RADIOISOTOPES ELECTRON CAPTURE RADIOISOTOPES INTERMEDIATE MASS NUCLEI Isotope Labeling - methods ISOTOPES LABELLED COMPOUNDS LABELLING MEDICINE Methods NUCLEAR MEDICINE NUCLEI ODD-EVEN NUCLEI RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY RADIOCHEMISTRY RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE SYNTHESIS Vitamin B 12 - chemical synthesis VITAMIN B GROUP VITAMIN B-12 VITAMINS 400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials |
| title | Preparation of Radioactive Labelled (57Co) Sulfitocobalamin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T06%3A34%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20of%20Radioactive%20Labelled%20(57Co)%20Sulfitocobalamin&rft.jtitle=Prep.%20Biochem.;%20(United%20States)&rft.au=Begley,%20James%20A.&rft.aucorp=Veterans%20Administration%20Hosp.,%20Albany,%20NY&rft.date=1978-01-01&rft.volume=8&rft.issue=6&rft.spage=411&rft.epage=419&rft.pages=411-419&rft.issn=0032-7484&rft_id=info:doi/10.1080/00327487808061659&rft_dat=%3Cproquest_pubme%3E74375708%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74375708&rft_id=info:pmid/748915&rfr_iscdi=true |