Determination of formaldehyde in biological tissues by gas chromatography/mass spectrometry
A quantitative method is described for the determination of formaldehyde in biological tissues by stable isotope dilution using gas chromatography/mass spectrometry. (13C2H2)Formaldehyde is used as the isotopic diluet. After tissue homogenization, derivatization is carried out in situ with pentafluo...
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| Veröffentlicht in: | Biomedical Mass Spectrometry 1982-08, Vol.9 (8), p.347-353 |
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| container_title | Biomedical Mass Spectrometry |
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| creator | d'A. Heck, Henry White, Earl L. Casanova‐Schmitz, Mercedes |
| description | A quantitative method is described for the determination of formaldehyde in biological tissues by stable isotope dilution using gas chromatography/mass spectrometry. (13C2H2)Formaldehyde is used as the isotopic diluet. After tissue homogenization, derivatization is carried out in situ with pentafluorophenylhydrazine, followed by extraction and analysis using selected ion monitoring. The sensitivity of the technique is higher than that of conventional methods of formaldehyde analysis, enabling endogenous formaldehyde to be quantitatively analyzed in tissues, even in samples as small as 20 mg wet weight. The effects of exposure to airborne formaldehyde or to airborne methyl chloride on the formaldehyde concentrations of several tissues of Fischer‐344 rats are reported. |
| doi_str_mv | 10.1002/bms.1200090808 |
| format | Article |
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Heck, Henry ; White, Earl L. ; Casanova‐Schmitz, Mercedes</creator><creatorcontrib>d'A. Heck, Henry ; White, Earl L. ; Casanova‐Schmitz, Mercedes</creatorcontrib><description>A quantitative method is described for the determination of formaldehyde in biological tissues by stable isotope dilution using gas chromatography/mass spectrometry. (13C2H2)Formaldehyde is used as the isotopic diluet. After tissue homogenization, derivatization is carried out in situ with pentafluorophenylhydrazine, followed by extraction and analysis using selected ion monitoring. The sensitivity of the technique is higher than that of conventional methods of formaldehyde analysis, enabling endogenous formaldehyde to be quantitatively analyzed in tissues, even in samples as small as 20 mg wet weight. The effects of exposure to airborne formaldehyde or to airborne methyl chloride on the formaldehyde concentrations of several tissues of Fischer‐344 rats are reported.</description><identifier>ISSN: 0306-042X</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/bms.1200090808</identifier><identifier>PMID: 7126766</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Brain Chemistry ; Carbon Isotopes ; Deuterium ; Formaldehyde - analysis ; Formaldehyde - metabolism ; Formaldehyde - toxicity ; Gas Chromatography-Mass Spectrometry ; Liver - analysis ; Male ; Methyl Chloride - metabolism ; Nasal Mucosa - analysis ; Rats ; Rats, Inbred F344 ; Testis - analysis</subject><ispartof>Biomedical Mass Spectrometry, 1982-08, Vol.9 (8), p.347-353</ispartof><rights>Copyright © 1982 Heyden & Son Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbms.1200090808$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbms.1200090808$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7126766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>d'A. Heck, Henry</creatorcontrib><creatorcontrib>White, Earl L.</creatorcontrib><creatorcontrib>Casanova‐Schmitz, Mercedes</creatorcontrib><title>Determination of formaldehyde in biological tissues by gas chromatography/mass spectrometry</title><title>Biomedical Mass Spectrometry</title><addtitle>Biomed Mass Spectrom</addtitle><description>A quantitative method is described for the determination of formaldehyde in biological tissues by stable isotope dilution using gas chromatography/mass spectrometry. (13C2H2)Formaldehyde is used as the isotopic diluet. After tissue homogenization, derivatization is carried out in situ with pentafluorophenylhydrazine, followed by extraction and analysis using selected ion monitoring. The sensitivity of the technique is higher than that of conventional methods of formaldehyde analysis, enabling endogenous formaldehyde to be quantitatively analyzed in tissues, even in samples as small as 20 mg wet weight. The effects of exposure to airborne formaldehyde or to airborne methyl chloride on the formaldehyde concentrations of several tissues of Fischer‐344 rats are reported.</description><subject>Animals</subject><subject>Brain Chemistry</subject><subject>Carbon Isotopes</subject><subject>Deuterium</subject><subject>Formaldehyde - analysis</subject><subject>Formaldehyde - metabolism</subject><subject>Formaldehyde - toxicity</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Liver - analysis</subject><subject>Male</subject><subject>Methyl Chloride - metabolism</subject><subject>Nasal Mucosa - analysis</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Testis - analysis</subject><issn>0306-042X</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRaq1evQn7B9LOZpvs7lFr_YCKBxUED2E2mW1XkiZkUyT_3kiLPQ0zDwzv-zB2LWAqAOKZrcJUxABgQIM-YWMBJo2M1vqUjUFCGsE8_jxnFyF8A0gtQI7YSIk4VWk6Zl_31FFb-S12vt7y2nFXtxWWBW36grjfcuvrsl77HEve-RB2FLjt-RoDzzdtXWFXr1tsNv2swhB4aCjvhjN1bX_JzhyWga4Oc8I-Hpbvi6do9fr4vLhdRY0YYkYECSCkhbWFlrFCbaQRuUVHyqrEaYnKJXEhEmcKpIQMgspTchaIVIxzOWE3-7_NzlZUZE3rK2z77FBy4GbPf3xJ_T8WkP0ZzAaD2dFgdvfydtzkL2XgZ9E</recordid><startdate>198208</startdate><enddate>198208</enddate><creator>d'A. Heck, Henry</creator><creator>White, Earl L.</creator><creator>Casanova‐Schmitz, Mercedes</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>198208</creationdate><title>Determination of formaldehyde in biological tissues by gas chromatography/mass spectrometry</title><author>d'A. 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Heck, Henry</creatorcontrib><creatorcontrib>White, Earl L.</creatorcontrib><creatorcontrib>Casanova‐Schmitz, Mercedes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Biomedical Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>d'A. Heck, Henry</au><au>White, Earl L.</au><au>Casanova‐Schmitz, Mercedes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of formaldehyde in biological tissues by gas chromatography/mass spectrometry</atitle><jtitle>Biomedical Mass Spectrometry</jtitle><addtitle>Biomed Mass Spectrom</addtitle><date>1982-08</date><risdate>1982</risdate><volume>9</volume><issue>8</issue><spage>347</spage><epage>353</epage><pages>347-353</pages><issn>0306-042X</issn><eissn>1096-9888</eissn><abstract>A quantitative method is described for the determination of formaldehyde in biological tissues by stable isotope dilution using gas chromatography/mass spectrometry. (13C2H2)Formaldehyde is used as the isotopic diluet. After tissue homogenization, derivatization is carried out in situ with pentafluorophenylhydrazine, followed by extraction and analysis using selected ion monitoring. The sensitivity of the technique is higher than that of conventional methods of formaldehyde analysis, enabling endogenous formaldehyde to be quantitatively analyzed in tissues, even in samples as small as 20 mg wet weight. The effects of exposure to airborne formaldehyde or to airborne methyl chloride on the formaldehyde concentrations of several tissues of Fischer‐344 rats are reported.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7126766</pmid><doi>10.1002/bms.1200090808</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-042X |
| ispartof | Biomedical Mass Spectrometry, 1982-08, Vol.9 (8), p.347-353 |
| issn | 0306-042X 1096-9888 |
| language | eng |
| recordid | cdi_pubmed_primary_7126766 |
| source | Wiley; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Brain Chemistry Carbon Isotopes Deuterium Formaldehyde - analysis Formaldehyde - metabolism Formaldehyde - toxicity Gas Chromatography-Mass Spectrometry Liver - analysis Male Methyl Chloride - metabolism Nasal Mucosa - analysis Rats Rats, Inbred F344 Testis - analysis |
| title | Determination of formaldehyde in biological tissues by gas chromatography/mass spectrometry |
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