Enzymatic Determination of Total 3α-Hydroxy Bile Acids in Faeces: Validation in Healthy Subjects of a Rapid Method Suitable for Clinical Routine Purpose
A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3α-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery...
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| Veröffentlicht in: | Scandinavian journal of gastroenterology 1982-04, Vol.17 (3), p.331-333 |
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| container_end_page | 333 |
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| container_issue | 3 |
| container_start_page | 331 |
| container_title | Scandinavian journal of gastroenterology |
| container_volume | 17 |
| creator | Malchow-Møller, A. Arffmann, S. Larusso, N. F. Krag, E. |
| description | A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3α-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h. |
| doi_str_mv | 10.3109/00365528209182063 |
| format | Article |
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F.</au><au>Krag, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Determination of Total 3α-Hydroxy Bile Acids in Faeces: Validation in Healthy Subjects of a Rapid Method Suitable for Clinical Routine Purpose</atitle><jtitle>Scandinavian journal of gastroenterology</jtitle><addtitle>Scand J Gastroenterol</addtitle><date>1982-04</date><risdate>1982</risdate><volume>17</volume><issue>3</issue><spage>331</spage><epage>333</epage><pages>331-333</pages><issn>0036-5521</issn><eissn>1502-7708</eissn><abstract>A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3α-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. 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| fulltext | fulltext |
| identifier | ISSN: 0036-5521 |
| ispartof | Scandinavian journal of gastroenterology, 1982-04, Vol.17 (3), p.331-333 |
| issn | 0036-5521 1502-7708 |
| language | eng |
| recordid | cdi_pubmed_primary_6957975 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | 3-Hydroxysteroid Dehydrogenases - analysis Adult Aged Bile acids Bile Acids and Salts - analysis Chromatography, Gas Clinical Enzyme Tests faeces Feces - analysis Feces - enzymology Humans Middle Aged |
| title | Enzymatic Determination of Total 3α-Hydroxy Bile Acids in Faeces: Validation in Healthy Subjects of a Rapid Method Suitable for Clinical Routine Purpose |
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