Quantitation of mRNAs during Mouse Spermatogenesis: Protamine-Like Histone and Phosphoglycerate Kinase-2 mRNAs Increase after Meiosis
Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-10, Vol.77 (10), p.6086-6090 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Erickson, Robert P. Kramer, James M. Rittenhouse, Judith Salkeld, Ann |
| description | Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis. |
| doi_str_mv | 10.1073/pnas.77.10.6086 |
| format | Article |
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Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.10.6086</identifier><identifier>PMID: 6934535</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Animals ; Cell separation ; Gels ; Histones - biosynthesis ; Histones - genetics ; Male ; Meiosis ; Messenger RNA ; Mice ; Phosphoglycerate Kinase - biosynthesis ; Phosphoglycerate Kinase - genetics ; Protamines - genetics ; RNA ; RNA, Messenger - metabolism ; Spermatids ; Spermatocytes ; Spermatogenesis ; Spermatozoa ; Testes ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-10, Vol.77 (10), p.6086-6090</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3756-dc17413ed5322ff6cd9ac4901b3a77e8ac0a314d8d1b4c25ff0c27df994843373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/9475$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/9475$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6934535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erickson, Robert P.</creatorcontrib><creatorcontrib>Kramer, James M.</creatorcontrib><creatorcontrib>Rittenhouse, Judith</creatorcontrib><creatorcontrib>Salkeld, Ann</creatorcontrib><title>Quantitation of mRNAs during Mouse Spermatogenesis: Protamine-Like Histone and Phosphoglycerate Kinase-2 mRNAs Increase after Meiosis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Cell separation</subject><subject>Gels</subject><subject>Histones - biosynthesis</subject><subject>Histones - genetics</subject><subject>Male</subject><subject>Meiosis</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>Phosphoglycerate Kinase - biosynthesis</subject><subject>Phosphoglycerate Kinase - genetics</subject><subject>Protamines - genetics</subject><subject>RNA</subject><subject>RNA, Messenger - metabolism</subject><subject>Spermatids</subject><subject>Spermatocytes</subject><subject>Spermatogenesis</subject><subject>Spermatozoa</subject><subject>Testes</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vFCEYxonR1LV6Nh40nPQ0WxiYYaaJh6ZR27jV-u9MWOZllzoDIzDGfgC_t0x23diLJwLP73l44UHoKSVLSgQ7GZ2KSyHyZlmTpr6HFpS0tKh5S-6jBSGlKBpe8ofoUYw3hJC2asgROqpbxitWLdDvT5NyySaVrHfYGzx8_nAWcTcF6zb4yk8R8JcRwqCS34CDaOMpvg4-qcE6KFb2O-ALG5N3gJXr8PXWx3HrN_2thqAS4Pc2TwhFuQ--dDpAPsDKJAj4CqzPkY_RA6P6CE_26zH69vbN1_OLYvXx3eX52arQTFR10WkqOGXQVawsjal11yqdX0rXTAkBjdJEMcq7pqNrrsvKGKJL0Zm25Q1nTLBj9HqXO07rAToNLgXVyzHYQYVb6ZWVdxVnt3Ljf0pWkZI22f9y7w_-xwQxycFGDX2vHOSvkqJivBGUZPBkB-rgYwxgDndQIufi5FycFGLez8Vlx_N_Rzvw-6ay_mKvz8a_6p2AV_8FpJn6PsGvlMlnO_Im9xYOaMvz-H8Awe-4Ww</recordid><startdate>19801001</startdate><enddate>19801001</enddate><creator>Erickson, Robert P.</creator><creator>Kramer, James M.</creator><creator>Rittenhouse, Judith</creator><creator>Salkeld, Ann</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801001</creationdate><title>Quantitation of mRNAs during Mouse Spermatogenesis: Protamine-Like Histone and Phosphoglycerate Kinase-2 mRNAs Increase after Meiosis</title><author>Erickson, Robert P. ; Kramer, James M. ; Rittenhouse, Judith ; Salkeld, Ann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3756-dc17413ed5322ff6cd9ac4901b3a77e8ac0a314d8d1b4c25ff0c27df994843373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Cell separation</topic><topic>Gels</topic><topic>Histones - biosynthesis</topic><topic>Histones - genetics</topic><topic>Male</topic><topic>Meiosis</topic><topic>Messenger RNA</topic><topic>Mice</topic><topic>Phosphoglycerate Kinase - biosynthesis</topic><topic>Phosphoglycerate Kinase - genetics</topic><topic>Protamines - genetics</topic><topic>RNA</topic><topic>RNA, Messenger - metabolism</topic><topic>Spermatids</topic><topic>Spermatocytes</topic><topic>Spermatogenesis</topic><topic>Spermatozoa</topic><topic>Testes</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erickson, Robert P.</creatorcontrib><creatorcontrib>Kramer, James M.</creatorcontrib><creatorcontrib>Rittenhouse, Judith</creatorcontrib><creatorcontrib>Salkeld, Ann</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erickson, Robert P.</au><au>Kramer, James M.</au><au>Rittenhouse, Judith</au><au>Salkeld, Ann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of mRNAs during Mouse Spermatogenesis: Protamine-Like Histone and Phosphoglycerate Kinase-2 mRNAs Increase after Meiosis</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-10-01</date><risdate>1980</risdate><volume>77</volume><issue>10</issue><spage>6086</spage><epage>6090</epage><pages>6086-6090</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6934535</pmid><doi>10.1073/pnas.77.10.6086</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; PubMed Central Free; MEDLINE; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Animals Cell separation Gels Histones - biosynthesis Histones - genetics Male Meiosis Messenger RNA Mice Phosphoglycerate Kinase - biosynthesis Phosphoglycerate Kinase - genetics Protamines - genetics RNA RNA, Messenger - metabolism Spermatids Spermatocytes Spermatogenesis Spermatozoa Testes Transcription, Genetic |
| title | Quantitation of mRNAs during Mouse Spermatogenesis: Protamine-Like Histone and Phosphoglycerate Kinase-2 mRNAs Increase after Meiosis |
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