Isolation of the Gene Encoding the Human T-lymphocyte Differentiation Antigen Leu-2 (T8) by Gene Transfer and cDNA Subtraction

We report the isolation of genomic and cDNA clones encoding the human T-lymphocyte cell-surface differentiation antigen, Leu-2 (T8), by use of a combination of transfection, fluorescence-activated cell-sorting, and subtractive cDNA hybridization. We constructed a cDNA library with mRNA from a mouse...

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