Use of the Escherichia coli lac Repressor and Operator to Control Gene Expression in Bacillus subtilis
The Escherichia coli lac operator has been placed on the 3′side of the promoter for the penicillinase gene of Bacillus licheniformis, creating a hybrid promoter controllable by the E. coli lac repressor. The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-bind...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (2), p.439-443 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Yansura, Daniel G. Henner, Dennis J. |
| description | The Escherichia coli lac operator has been placed on the 3′side of the promoter for the penicillinase gene of Bacillus licheniformis, creating a hybrid promoter controllable by the E. coli lac repressor. The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-binding site that allows expression in Bacillus subtilis. When the penicillinase gene that contains the lac operator is expressed in B. subtilis on a plasmid that also produces the lac repressor, the expression of the penicillinase gene can be modulated by isopropyl β -D-thiogalactoside (IPTG), an inducer of the lac operon in E. coli. A similar system was constructed from a promoter of the B. subtilis phage SPO-1 and the leukocyte interferon A gene, which allowed the controlled expression of interferon in B. subtilis. These two examples show that a functional control system can be introduced into B. subtilis from E. coli. |
| doi_str_mv | 10.1073/pnas.81.2.439 |
| format | Article |
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The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-binding site that allows expression in Bacillus subtilis. When the penicillinase gene that contains the lac operator is expressed in B. subtilis on a plasmid that also produces the lac repressor, the expression of the penicillinase gene can be modulated by isopropyl β -D-thiogalactoside (IPTG), an inducer of the lac operon in E. coli. A similar system was constructed from a promoter of the B. subtilis phage SPO-1 and the leukocyte interferon A gene, which allowed the controlled expression of interferon in B. subtilis. 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The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-binding site that allows expression in Bacillus subtilis. When the penicillinase gene that contains the lac operator is expressed in B. subtilis on a plasmid that also produces the lac repressor, the expression of the penicillinase gene can be modulated by isopropyl β -D-thiogalactoside (IPTG), an inducer of the lac operon in E. coli. A similar system was constructed from a promoter of the B. subtilis phage SPO-1 and the leukocyte interferon A gene, which allowed the controlled expression of interferon in B. subtilis. These two examples show that a functional control system can be introduced into B. subtilis from E. coli.</description><subject>Bacillus - genetics</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>DNA</subject><subject>DNA, Recombinant</subject><subject>Enzyme Induction</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genetic vectors</subject><subject>Hybridity</subject><subject>Interferon Type I - genetics</subject><subject>Interferons</subject><subject>Leukocytes</subject><subject>operators</subject><subject>Operon</subject><subject>Penicillinase - genetics</subject><subject>Plasmids</subject><subject>Promoter regions</subject><subject>Repressor proteins</subject><subject>Repressor Proteins - genetics</subject><subject>repressors</subject><subject>RNA</subject><subject>Transcription Factors - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo67h69CIKueitx3x1Ojl40GFdhYUFcc8hk6k4WTKdNknL-t-bccZhveipKN7vFa94CD2nZEnJwN9Ooy1LRZdsKbh-gBaUaNpJoclDtCCEDZ0STDxGT0q5JYToXpEzdCYFI4PSC-RvCuDkcd0CvihuCzm4bbDYpRhwtA5_gSlDKSljO27w9QTZ1rbUhFdprDlFfAlj8979xkIacRjxB-tCjHPBZV7XEEN5ih55Gws8O85zdPPx4uvqU3d1ffl59f6qc70StdtQ2VKBtmvBvfLSeu8t58z3XHLmOFEU1nrwzjolhFS9VAPVwBkflJAe-Dl6d7g7zesdbBy0iDaaKYedzT9NssH8rYxha76lH4a3c5o1_5ujP6fvM5RqdqE4iNGOkOZiFNFCCEX-C1KuSK_6PdgdQJdTKRn8KQwlZl-g2RdoFDXMtAIb_-r-Byf62FjTXx71ve2Pes_--h-y8XOMFe5q414cuNvS6jyBjEmt-C9X0LiF</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Yansura, Daniel G.</creator><creator>Henner, Dennis J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840101</creationdate><title>Use of the Escherichia coli lac Repressor and Operator to Control Gene Expression in Bacillus subtilis</title><author>Yansura, Daniel G. ; Henner, Dennis J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-d16789e9ab43f8f6afffa332f53632c3081eb97fcac84468568719e3237846fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Bacillus - genetics</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>DNA</topic><topic>DNA, Recombinant</topic><topic>Enzyme Induction</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genetic vectors</topic><topic>Hybridity</topic><topic>Interferon Type I - genetics</topic><topic>Interferons</topic><topic>Leukocytes</topic><topic>operators</topic><topic>Operon</topic><topic>Penicillinase - genetics</topic><topic>Plasmids</topic><topic>Promoter regions</topic><topic>Repressor proteins</topic><topic>Repressor Proteins - genetics</topic><topic>repressors</topic><topic>RNA</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yansura, Daniel G.</creatorcontrib><creatorcontrib>Henner, Dennis J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yansura, Daniel G.</au><au>Henner, Dennis J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of the Escherichia coli lac Repressor and Operator to Control Gene Expression in Bacillus subtilis</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>81</volume><issue>2</issue><spage>439</spage><epage>443</epage><pages>439-443</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The Escherichia coli lac operator has been placed on the 3′side of the promoter for the penicillinase gene of Bacillus licheniformis, creating a hybrid promoter controllable by the E. coli lac repressor. The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-binding site that allows expression in Bacillus subtilis. When the penicillinase gene that contains the lac operator is expressed in B. subtilis on a plasmid that also produces the lac repressor, the expression of the penicillinase gene can be modulated by isopropyl β -D-thiogalactoside (IPTG), an inducer of the lac operon in E. coli. A similar system was constructed from a promoter of the B. subtilis phage SPO-1 and the leukocyte interferon A gene, which allowed the controlled expression of interferon in B. subtilis. These two examples show that a functional control system can be introduced into B. subtilis from E. coli.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6420789</pmid><doi>10.1073/pnas.81.2.439</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1984-01, Vol.81 (2), p.439-443 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR |
| subjects | Bacillus - genetics Bacillus subtilis Bacillus subtilis - genetics DNA DNA, Recombinant Enzyme Induction Escherichia coli Escherichia coli - genetics gene expression Gene Expression Regulation Genes Genetic vectors Hybridity Interferon Type I - genetics Interferons Leukocytes operators Operon Penicillinase - genetics Plasmids Promoter regions Repressor proteins Repressor Proteins - genetics repressors RNA Transcription Factors - genetics |
| title | Use of the Escherichia coli lac Repressor and Operator to Control Gene Expression in Bacillus subtilis |
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