Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes
The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a sy...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1984-03, Vol.81 (6), p.1784-1788 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1788 |
|---|---|
| container_issue | 6 |
| container_start_page | 1784 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 81 |
| creator | Chen, Pojen P. Houghten, Richard A. Fong, Sherman Rhodes, Gary H. Gilbertson, Timothy A. Vaughan, John H. Lerner, Richard A. Carson, Dennis A. |
| description | The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific antiidiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes. |
| doi_str_mv | 10.1073/pnas.81.6.1784 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmed_primary_6200884</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23346</jstor_id><sourcerecordid>23346</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-f61a83fd1a61f7b0054f8d4c165179817d3f5bf148057ce58d804ca0cb05df733</originalsourceid><addsrcrecordid>eNp9kUuP0zAUhS0EGsrAlgUSklezS7huHMdFYlGVx1QaCcRjbTl-tB6lcbCdEdnx03HUUpUNq6ur8517rnQQekmgJNBUb4ZexpKTkpWk4fQRWhBYkYLRFTxGC4BlU3C6pE_RsxjvAWBVc7hCV2wJwDldoN_rPrnidhpMeJDBybYz-KvZOd_jWWm9nvC216MyGrcTlvi9sa7PyxczJKfN24zh9TAEL9UeWx_wtzTqyfU7nPYmL2FUaQyywxsfgulkMhF7i7fa-ZRT43P0xMoumheneY1-fPzwfXNb3H3-tN2s7wpFa5YKy4jkldVEMmKbFqCmlmuqCKtJs-Kk0ZWtW0soh7pRpuaaA1USVAu1tk1VXaN3x7vD2B6MVqZP-SsxBHeQYRJeOvGv0ru92PkHUdE6p2X_zckf_M_RxCQOLirTdbI3foyCE8gFsCaD5RFUwccYjD1nEBBzZWKuLPOCibmybHh9-dkZP3V0oc--v-ql_-Z_urBj1yXzK2Xw1RG8j8mHM7msKsqqPw9NtX8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81017867</pqid></control><display><type>article</type><title>Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Chen, Pojen P. ; Houghten, Richard A. ; Fong, Sherman ; Rhodes, Gary H. ; Gilbertson, Timothy A. ; Vaughan, John H. ; Lerner, Richard A. ; Carson, Dennis A.</creator><creatorcontrib>Chen, Pojen P. ; Houghten, Richard A. ; Fong, Sherman ; Rhodes, Gary H. ; Gilbertson, Timothy A. ; Vaughan, John H. ; Lerner, Richard A. ; Carson, Dennis A.</creatorcontrib><description>The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific antiidiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.81.6.1784</identifier><identifier>PMID: 6200884</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Animals ; Anti idiotypic antibodies ; Antibodies ; Antibodies, Anti-Idiotypic - immunology ; Antibody Formation ; Antigens ; Epitopes ; Immunoglobulin G - immunology ; Immunoglobulin Idiotypes - immunology ; Immunoglobulin M - immunology ; Immunoglobulin Variable Region - immunology ; Immunoglobulins ; Molecules ; Paraproteins ; Peptide Fragments - immunology ; Rabbits ; Room temperature ; Serum albumins ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1984-03, Vol.81 (6), p.1784-1788</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-f61a83fd1a61f7b0054f8d4c165179817d3f5bf148057ce58d804ca0cb05df733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/81/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23346$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23346$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6200884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Pojen P.</creatorcontrib><creatorcontrib>Houghten, Richard A.</creatorcontrib><creatorcontrib>Fong, Sherman</creatorcontrib><creatorcontrib>Rhodes, Gary H.</creatorcontrib><creatorcontrib>Gilbertson, Timothy A.</creatorcontrib><creatorcontrib>Vaughan, John H.</creatorcontrib><creatorcontrib>Lerner, Richard A.</creatorcontrib><creatorcontrib>Carson, Dennis A.</creatorcontrib><title>Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific antiidiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Anti idiotypic antibodies</subject><subject>Antibodies</subject><subject>Antibodies, Anti-Idiotypic - immunology</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Epitopes</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin Idiotypes - immunology</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunoglobulin Variable Region - immunology</subject><subject>Immunoglobulins</subject><subject>Molecules</subject><subject>Paraproteins</subject><subject>Peptide Fragments - immunology</subject><subject>Rabbits</subject><subject>Room temperature</subject><subject>Serum albumins</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuP0zAUhS0EGsrAlgUSklezS7huHMdFYlGVx1QaCcRjbTl-tB6lcbCdEdnx03HUUpUNq6ur8517rnQQekmgJNBUb4ZexpKTkpWk4fQRWhBYkYLRFTxGC4BlU3C6pE_RsxjvAWBVc7hCV2wJwDldoN_rPrnidhpMeJDBybYz-KvZOd_jWWm9nvC216MyGrcTlvi9sa7PyxczJKfN24zh9TAEL9UeWx_wtzTqyfU7nPYmL2FUaQyywxsfgulkMhF7i7fa-ZRT43P0xMoumheneY1-fPzwfXNb3H3-tN2s7wpFa5YKy4jkldVEMmKbFqCmlmuqCKtJs-Kk0ZWtW0soh7pRpuaaA1USVAu1tk1VXaN3x7vD2B6MVqZP-SsxBHeQYRJeOvGv0ru92PkHUdE6p2X_zckf_M_RxCQOLirTdbI3foyCE8gFsCaD5RFUwccYjD1nEBBzZWKuLPOCibmybHh9-dkZP3V0oc--v-ql_-Z_urBj1yXzK2Xw1RG8j8mHM7msKsqqPw9NtX8</recordid><startdate>19840301</startdate><enddate>19840301</enddate><creator>Chen, Pojen P.</creator><creator>Houghten, Richard A.</creator><creator>Fong, Sherman</creator><creator>Rhodes, Gary H.</creator><creator>Gilbertson, Timothy A.</creator><creator>Vaughan, John H.</creator><creator>Lerner, Richard A.</creator><creator>Carson, Dennis A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840301</creationdate><title>Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes</title><author>Chen, Pojen P. ; Houghten, Richard A. ; Fong, Sherman ; Rhodes, Gary H. ; Gilbertson, Timothy A. ; Vaughan, John H. ; Lerner, Richard A. ; Carson, Dennis A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-f61a83fd1a61f7b0054f8d4c165179817d3f5bf148057ce58d804ca0cb05df733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Anti idiotypic antibodies</topic><topic>Antibodies</topic><topic>Antibodies, Anti-Idiotypic - immunology</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Epitopes</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin Idiotypes - immunology</topic><topic>Immunoglobulin M - immunology</topic><topic>Immunoglobulin Variable Region - immunology</topic><topic>Immunoglobulins</topic><topic>Molecules</topic><topic>Paraproteins</topic><topic>Peptide Fragments - immunology</topic><topic>Rabbits</topic><topic>Room temperature</topic><topic>Serum albumins</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Pojen P.</creatorcontrib><creatorcontrib>Houghten, Richard A.</creatorcontrib><creatorcontrib>Fong, Sherman</creatorcontrib><creatorcontrib>Rhodes, Gary H.</creatorcontrib><creatorcontrib>Gilbertson, Timothy A.</creatorcontrib><creatorcontrib>Vaughan, John H.</creatorcontrib><creatorcontrib>Lerner, Richard A.</creatorcontrib><creatorcontrib>Carson, Dennis A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Pojen P.</au><au>Houghten, Richard A.</au><au>Fong, Sherman</au><au>Rhodes, Gary H.</au><au>Gilbertson, Timothy A.</au><au>Vaughan, John H.</au><au>Lerner, Richard A.</au><au>Carson, Dennis A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1984-03-01</date><risdate>1984</risdate><volume>81</volume><issue>6</issue><spage>1784</spage><epage>1788</epage><pages>1784-1788</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific antiidiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6200884</pmid><doi>10.1073/pnas.81.6.1784</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1984-03, Vol.81 (6), p.1784-1788 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_6200884 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Animals Anti idiotypic antibodies Antibodies Antibodies, Anti-Idiotypic - immunology Antibody Formation Antigens Epitopes Immunoglobulin G - immunology Immunoglobulin Idiotypes - immunology Immunoglobulin M - immunology Immunoglobulin Variable Region - immunology Immunoglobulins Molecules Paraproteins Peptide Fragments - immunology Rabbits Room temperature Serum albumins Ungulates |
| title | Anti-Hypervariable Region Antibody Induced by a Defined Peptide: An Approach for Studying the Structural Correlates of Idiotypes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T17%3A52%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anti-Hypervariable%20Region%20Antibody%20Induced%20by%20a%20Defined%20Peptide:%20An%20Approach%20for%20Studying%20the%20Structural%20Correlates%20of%20Idiotypes&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Chen,%20Pojen%20P.&rft.date=1984-03-01&rft.volume=81&rft.issue=6&rft.spage=1784&rft.epage=1788&rft.pages=1784-1788&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.81.6.1784&rft_dat=%3Cjstor_pubme%3E23346%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81017867&rft_id=info:pmid/6200884&rft_jstor_id=23346&rfr_iscdi=true |