Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies)
The authors describe a 70-year-old woman with multiple myeloma and adult Fanconi syndrome. A monoclonal protein of IgA heavy-chain class and kappa light-chain class was demonstrable in the serum. Urine immunoelectrophoresis showed the presence of kappa light chains. Bone marrow aspirate showed incre...
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| Veröffentlicht in: | American journal of clinical pathology 1983-01, Vol.80 (2), p.224-228 |
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| container_title | American journal of clinical pathology |
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| creator | RAMAN, S. B. K VAN SLYCK, E. J |
| description | The authors describe a 70-year-old woman with multiple myeloma and adult Fanconi syndrome. A monoclonal protein of IgA heavy-chain class and kappa light-chain class was demonstrable in the serum. Urine immunoelectrophoresis showed the presence of kappa light chains. Bone marrow aspirate showed increased plasma cells with large bundles of pink-staining Auer-rod-like crystals in their cytoplasm. These crystals failed to stain with Sudan black B, peroxidase, esterase, and PAS, but showed strong acid phosphatase and beta-glucuronidase positivity. Ultrastructural studies showed them to have a fibrillar and an unusual cross-striated pattern. Immunofluorescent studies showed strong IgA and kappa activity in the cytoplasm of the tumor cells, but the fluorescence was absent in the region of the crystals, which were identified easily by their negative birefringence. The authors interpret these observations to indicate that the intracytoplasmic crystals in this case are of lysosomal origin. |
| doi_str_mv | 10.1093/ajcp/80.2.224 |
| format | Article |
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B. K ; VAN SLYCK, E. J</creator><creatorcontrib>RAMAN, S. B. K ; VAN SLYCK, E. J</creatorcontrib><description>The authors describe a 70-year-old woman with multiple myeloma and adult Fanconi syndrome. A monoclonal protein of IgA heavy-chain class and kappa light-chain class was demonstrable in the serum. Urine immunoelectrophoresis showed the presence of kappa light chains. Bone marrow aspirate showed increased plasma cells with large bundles of pink-staining Auer-rod-like crystals in their cytoplasm. These crystals failed to stain with Sudan black B, peroxidase, esterase, and PAS, but showed strong acid phosphatase and beta-glucuronidase positivity. Ultrastructural studies showed them to have a fibrillar and an unusual cross-striated pattern. Immunofluorescent studies showed strong IgA and kappa activity in the cytoplasm of the tumor cells, but the fluorescence was absent in the region of the crystals, which were identified easily by their negative birefringence. The authors interpret these observations to indicate that the intracytoplasmic crystals in this case are of lysosomal origin.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/80.2.224</identifier><identifier>PMID: 6192701</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Aged ; Biological and medical sciences ; Bone Marrow - ultrastructure ; Cytoplasmic Granules - ultrastructure ; Fanconi Syndrome - complications ; Female ; Fluorescent Antibody Technique ; Histocytochemistry ; Humans ; Immunodeficiencies. Immunoglobulinopathies ; Immunoglobulin A - analysis ; Immunoglobulin Heavy Chains - analysis ; Immunoglobulin kappa-Chains - analysis ; Immunoglobulinopathies ; Immunopathology ; Lysosomes - ultrastructure ; Medical sciences ; Multiple Myeloma - complications ; Multiple Myeloma - ultrastructure ; Plasma Cells - ultrastructure ; Staining and Labeling</subject><ispartof>American journal of clinical pathology, 1983-01, Vol.80 (2), p.224-228</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9461511$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6192701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RAMAN, S. B. K</creatorcontrib><creatorcontrib>VAN SLYCK, E. J</creatorcontrib><title>Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies)</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>The authors describe a 70-year-old woman with multiple myeloma and adult Fanconi syndrome. A monoclonal protein of IgA heavy-chain class and kappa light-chain class was demonstrable in the serum. Urine immunoelectrophoresis showed the presence of kappa light chains. Bone marrow aspirate showed increased plasma cells with large bundles of pink-staining Auer-rod-like crystals in their cytoplasm. These crystals failed to stain with Sudan black B, peroxidase, esterase, and PAS, but showed strong acid phosphatase and beta-glucuronidase positivity. Ultrastructural studies showed them to have a fibrillar and an unusual cross-striated pattern. Immunofluorescent studies showed strong IgA and kappa activity in the cytoplasm of the tumor cells, but the fluorescence was absent in the region of the crystals, which were identified easily by their negative birefringence. The authors interpret these observations to indicate that the intracytoplasmic crystals in this case are of lysosomal origin.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - ultrastructure</subject><subject>Cytoplasmic Granules - ultrastructure</subject><subject>Fanconi Syndrome - complications</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin Heavy Chains - analysis</subject><subject>Immunoglobulin kappa-Chains - analysis</subject><subject>Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Lysosomes - ultrastructure</subject><subject>Medical sciences</subject><subject>Multiple Myeloma - complications</subject><subject>Multiple Myeloma - ultrastructure</subject><subject>Plasma Cells - ultrastructure</subject><subject>Staining and Labeling</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMotVaPHoUcPCh023zt11GKX1D0oucyzc7alGSzJLuH_gl_sxGLpxnmGZ6XGUKuOVtwVssl7HW_rNhCLIRQJ2TKayWzshTilEwZYyKreSnPyUWMe8a4qJiakEnBa1EyPiXfbzCMAalvqemGAPow-N5CdEZTHQ5xAGtNhwlqO0bju5ha6g5ovQOq0dpI75wP_c5b_2X0nP4a9A6TAOycjjZJ4xBGnWJ-B9A11Dg3dr61ow8YNXYDjcPYGIz3l-SsBRvx6lhn5PPp8WP1kq3fn19XD-usF0wNmRIVh7wuAVWOZV41Tctz3oJq2irPVSuR50UhG5BYa4kMWFEpBtucI9uiZnJGbv68_bh12Gz6YByEw-b4l8RvjxxiuqMN0GkT_9dqVaQ8Ln8A0Fh2Sg</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>RAMAN, S. B. K</creator><creator>VAN SLYCK, E. J</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19830101</creationdate><title>Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies)</title><author>RAMAN, S. B. K ; VAN SLYCK, E. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p204t-4281a597ae45e758ddf151fa4df8554f3e15663da3e9c3e0a06840ab51e0bec03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - ultrastructure</topic><topic>Cytoplasmic Granules - ultrastructure</topic><topic>Fanconi Syndrome - complications</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin Heavy Chains - analysis</topic><topic>Immunoglobulin kappa-Chains - analysis</topic><topic>Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Lysosomes - ultrastructure</topic><topic>Medical sciences</topic><topic>Multiple Myeloma - complications</topic><topic>Multiple Myeloma - ultrastructure</topic><topic>Plasma Cells - ultrastructure</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAMAN, S. B. K</creatorcontrib><creatorcontrib>VAN SLYCK, E. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAMAN, S. B. K</au><au>VAN SLYCK, E. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies)</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>80</volume><issue>2</issue><spage>224</spage><epage>228</epage><pages>224-228</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>The authors describe a 70-year-old woman with multiple myeloma and adult Fanconi syndrome. A monoclonal protein of IgA heavy-chain class and kappa light-chain class was demonstrable in the serum. Urine immunoelectrophoresis showed the presence of kappa light chains. Bone marrow aspirate showed increased plasma cells with large bundles of pink-staining Auer-rod-like crystals in their cytoplasm. These crystals failed to stain with Sudan black B, peroxidase, esterase, and PAS, but showed strong acid phosphatase and beta-glucuronidase positivity. Ultrastructural studies showed them to have a fibrillar and an unusual cross-striated pattern. Immunofluorescent studies showed strong IgA and kappa activity in the cytoplasm of the tumor cells, but the fluorescence was absent in the region of the crystals, which were identified easily by their negative birefringence. The authors interpret these observations to indicate that the intracytoplasmic crystals in this case are of lysosomal origin.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>6192701</pmid><doi>10.1093/ajcp/80.2.224</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 1983-01, Vol.80 (2), p.224-228 |
| issn | 0002-9173 1943-7722 |
| language | eng |
| recordid | cdi_pubmed_primary_6192701 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Aged Biological and medical sciences Bone Marrow - ultrastructure Cytoplasmic Granules - ultrastructure Fanconi Syndrome - complications Female Fluorescent Antibody Technique Histocytochemistry Humans Immunodeficiencies. Immunoglobulinopathies Immunoglobulin A - analysis Immunoglobulin Heavy Chains - analysis Immunoglobulin kappa-Chains - analysis Immunoglobulinopathies Immunopathology Lysosomes - ultrastructure Medical sciences Multiple Myeloma - complications Multiple Myeloma - ultrastructure Plasma Cells - ultrastructure Staining and Labeling |
| title | Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies) |
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