A Method for the Gross and Microscopic Investigation of Vascular Reperfusion in Ischemic Myocardium
Fluorescein-isothiocyanate dextran (FITC-dextran; MW ∼ 70,000) was used in isolated rat hearts to compare normal vascular perfusion of ventricular myocardium with the pattern and extent of reperfusion following 60 minutes of global ischemia. Its gross distribution in frozen transverse sections throu...
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| Veröffentlicht in: | Biotechnic & histochemistry 1982-01, Vol.57 (5), p.301-305 |
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| container_title | Biotechnic & histochemistry |
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| creator | Mawson, J. B. Gavin, J. B. Seelye, R. N. Herdson, P. B. |
| description | Fluorescein-isothiocyanate dextran (FITC-dextran; MW ∼ 70,000) was used in isolated rat hearts to compare normal vascular perfusion of ventricular myocardium with the pattern and extent of reperfusion following 60 minutes of global ischemia. Its gross distribution in frozen transverse sections through the ventricles was similar to that of sodium fluorescein. However, unlike 0.1% sodium fluorescein, 6.7% FITC-dextran has a viscosity similar to that of blood, and its much higher molecular weight prevents its diffusion beyond the ischemically injured vessels. Furthermore, staining by the alcoholic periodic acid-Schiff technique enabled tracer distribution to be confirmed microscopically and distinguished competent from incompetent vessels in paraffin embedded material. |
| doi_str_mv | 10.3109/10520298209066727 |
| format | Article |
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Furthermore, staining by the alcoholic periodic acid-Schiff technique enabled tracer distribution to be confirmed microscopically and distinguished competent from incompetent vessels in paraffin embedded material.</description><subject>Animals</subject><subject>Coronary Disease - diagnosis</subject><subject>Coronary Disease - physiopathology</subject><subject>Coronary Vessels - physiopathology</subject><subject>Dextrans</subject><subject>Fluorescein-5-isothiocyanate - analogs & derivatives</subject><subject>Fluoresceins</subject><subject>Frozen Sections</subject><subject>In Vitro Techniques</subject><subject>Perfusion</subject><subject>Periodic Acid-Schiff Reaction</subject><subject>Rats</subject><subject>Regional Blood Flow</subject><issn>1052-0295</issn><issn>0038-9153</issn><issn>1473-7760</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMo4_UBXAhZuasmaZs06EbEy4CDIOq2pOmJzdA2Y9Iq8_ZmmEEQ0VUO-S-c8yF0TMlZSok8pyRnhMmCEUk4F0xsoT2aiTQRgpPtOEc9iYZ8F-2HMCeEiELSCZpwWmRFXuwhfYVnMDSuxsZ5PDSA77wLAau-xjOr46zdwmo87T8gDPZNDdb12Bn8qoIeW-XxEyzAmzGs_m2Pp0E30MXEbOm08rUdu0O0Y1Qb4GjzHqCX25vn6_vk4fFuen31kOhM0CEBA7mkuQBeGaYIlzRjumJCGilppSpuTFpzpjjNi5QYaQwTdS4UpDzLqeHpATpd9y68ex_jumVng4a2VT24MZQFYWnGOItGujau7gseTLnwtlN-WVJSrsCWv8DGzMmmfKw6qL8TG5JRv1zrto8kO_XpfFuXg1q2zhuvem3Dqvrv-osf8QZUOzQRIJRzN_o-cvtnuS_X9pj_</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Mawson, J. 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B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Method for the Gross and Microscopic Investigation of Vascular Reperfusion in Ischemic Myocardium</atitle><jtitle>Biotechnic & histochemistry</jtitle><addtitle>Stain Technol</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>57</volume><issue>5</issue><spage>301</spage><epage>305</epage><pages>301-305</pages><issn>1052-0295</issn><issn>0038-9153</issn><eissn>1473-7760</eissn><abstract>Fluorescein-isothiocyanate dextran (FITC-dextran; MW ∼ 70,000) was used in isolated rat hearts to compare normal vascular perfusion of ventricular myocardium with the pattern and extent of reperfusion following 60 minutes of global ischemia. Its gross distribution in frozen transverse sections through the ventricles was similar to that of sodium fluorescein. However, unlike 0.1% sodium fluorescein, 6.7% FITC-dextran has a viscosity similar to that of blood, and its much higher molecular weight prevents its diffusion beyond the ischemically injured vessels. Furthermore, staining by the alcoholic periodic acid-Schiff technique enabled tracer distribution to be confirmed microscopically and distinguished competent from incompetent vessels in paraffin embedded material.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>6184858</pmid><doi>10.3109/10520298209066727</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1052-0295 |
| ispartof | Biotechnic & histochemistry, 1982-01, Vol.57 (5), p.301-305 |
| issn | 1052-0295 0038-9153 1473-7760 |
| language | eng |
| recordid | cdi_pubmed_primary_6184858 |
| source | MEDLINE; Taylor & Francis Journals Complete |
| subjects | Animals Coronary Disease - diagnosis Coronary Disease - physiopathology Coronary Vessels - physiopathology Dextrans Fluorescein-5-isothiocyanate - analogs & derivatives Fluoresceins Frozen Sections In Vitro Techniques Perfusion Periodic Acid-Schiff Reaction Rats Regional Blood Flow |
| title | A Method for the Gross and Microscopic Investigation of Vascular Reperfusion in Ischemic Myocardium |
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