Development of a Sensitive Serological Assay Based on Reversed Passive Hemagglutination for Detection of Enteropathogenic Toxin (Kanagawa Hemolysin) of Vibrio parahaemolyticus, and Re-evaluation of the Toxin Producibility of Isolates from Various Sources

Development of a Sensitive Serological Assay Based on Reversed Passive Hemagglutination for Detection of Enteropathogenic Toxin (Kanagawa Hemolysin) of Vibrio parahaemolyticus, and Re-evaluation of the Toxin Producibility of Isolates from Various Sources

Based on reversed passive hemagglutination, a sensitive and specific serological assay was developed for detection of enteropathogenic toxin produced by Vibrio parahaemolyticus. It was so sensitive as to make the detection of 1ng/ml of the toxin practicable. By using the assay, vibrios isolated from...

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Veröffentlicht in:Nihon saikingaku zasshi 1979/11/25, Vol.34(6), pp.837-846
Hauptverfasser: OHTA, Kenji, KUDOH, Yasuo, TSUNO, Masaaki, SAKAI, Senzo, MARUYAMA, Tsutomu, ITOH, Takeshi, OHASHI, Makoto
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Bacterial Toxins - analysis
Foodborne Diseases - microbiology
Hemagglutination Tests - methods
Hemolysin Proteins - analysis
Humans
Vibrio parahaemolyticus - metabolism
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container_end_page 846
container_issue 6
container_start_page 837
container_title Nihon saikingaku zasshi
container_volume 34
creator OHTA, Kenji
KUDOH, Yasuo
TSUNO, Masaaki
SAKAI, Senzo
MARUYAMA, Tsutomu
ITOH, Takeshi
OHASHI, Makoto
description Based on reversed passive hemagglutination, a sensitive and specific serological assay was developed for detection of enteropathogenic toxin produced by Vibrio parahaemolyticus. It was so sensitive as to make the detection of 1ng/ml of the toxin practicable. By using the assay, vibrios isolated from various sources were examined for toxin-produ-cibility. Kanagawa phenomenon-positive isolates of human origin produced more than 1μg/ml of the toxin. Most of the Kanagawa-negative and Kanagawa-questionable isolates from fecal specimens of gastroenteritis patients produced a smaller amount of the toxin. Those obtained from seafish, shellfish or their cooking utensils did not produce any detectable amount of the toxin. Based on these findings, the role of the toxin was discussed in causing diarrhea in the case of gastroenteritis due to Kanagawa-negative Vibrio parahaemolyticus.
doi_str_mv 10.3412/jsb.34.837
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1882-4110
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source J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals
subjects Bacterial Toxins - analysis
Foodborne Diseases - microbiology
Hemagglutination Tests - methods
Hemolysin Proteins - analysis
Humans
Vibrio parahaemolyticus - metabolism
title Development of a Sensitive Serological Assay Based on Reversed Passive Hemagglutination for Detection of Enteropathogenic Toxin (Kanagawa Hemolysin) of Vibrio parahaemolyticus, and Re-evaluation of the Toxin Producibility of Isolates from Various Sources
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