The Effect of Altering the Size of Poly C on the Toxicity and Antigenicity of Poly I:C
Summary Appropriate reduction in size of poly C by degradation with ribonuclease prior to complexing with poly I effected marked reduction in toxicity for mice and antigenicity for rabbits of poly I:C without detectable reduction in in vivo or in vitro protection against viral infection. There was s...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1972-12, Vol.141 (3), p.1068-1072 |
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| container_end_page | 1072 |
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| container_issue | 3 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 141 |
| creator | Lampson, George P. Nemes, Marjorie M. Field, A. Kirk Tytell, Alfred A. Hilleman, Maurice R. |
| description | Summary
Appropriate reduction in size of poly C by degradation with ribonuclease prior to complexing with poly I effected marked reduction in toxicity for mice and antigenicity for rabbits of poly I:C without detectable reduction in in vivo or in vitro protection against viral infection. There was slight reduction in interferon-inducing capacity for rabbits. Such procedure adds another possible means for increasing the safety of poly I:C in eventual clinical application.
The authors are indebted to Mary-Ellen Davies, Helen Perry, Carolyn Saydah and Carol Bonoma for excellent technical assistance. |
| doi_str_mv | 10.3181/00379727-141-36934 |
| format | Article |
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Appropriate reduction in size of poly C by degradation with ribonuclease prior to complexing with poly I effected marked reduction in toxicity for mice and antigenicity for rabbits of poly I:C without detectable reduction in in vivo or in vitro protection against viral infection. There was slight reduction in interferon-inducing capacity for rabbits. Such procedure adds another possible means for increasing the safety of poly I:C in eventual clinical application.
The authors are indebted to Mary-Ellen Davies, Helen Perry, Carolyn Saydah and Carol Bonoma for excellent technical assistance.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-141-36934</identifier><identifier>PMID: 4645754</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Antibodies - analysis ; Antigens ; Biological Assay ; Chromatography, Gel ; Cytosine Nucleotides - analysis ; Injections, Intraperitoneal ; Injections, Intravenous ; Interferon Inducers ; Mice ; Molecular Weight ; Poly I-C - administration & dosage ; Poly I-C - analysis ; Poly I-C - chemical synthesis ; Poly I-C - toxicity ; Polynucleotides - analysis ; Rabbits ; Ribonucleases - metabolism</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1972-12, Vol.141 (3), p.1068-1072</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-3ab57e233cd2c11b79bf452913b0b1bce4df6fb2bae1999fc351e957452c53573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4645754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lampson, George P.</creatorcontrib><creatorcontrib>Nemes, Marjorie M.</creatorcontrib><creatorcontrib>Field, A. Kirk</creatorcontrib><creatorcontrib>Tytell, Alfred A.</creatorcontrib><creatorcontrib>Hilleman, Maurice R.</creatorcontrib><title>The Effect of Altering the Size of Poly C on the Toxicity and Antigenicity of Poly I:C</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Appropriate reduction in size of poly C by degradation with ribonuclease prior to complexing with poly I effected marked reduction in toxicity for mice and antigenicity for rabbits of poly I:C without detectable reduction in in vivo or in vitro protection against viral infection. There was slight reduction in interferon-inducing capacity for rabbits. Such procedure adds another possible means for increasing the safety of poly I:C in eventual clinical application.
The authors are indebted to Mary-Ellen Davies, Helen Perry, Carolyn Saydah and Carol Bonoma for excellent technical assistance.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antigens</subject><subject>Biological Assay</subject><subject>Chromatography, Gel</subject><subject>Cytosine Nucleotides - analysis</subject><subject>Injections, Intraperitoneal</subject><subject>Injections, Intravenous</subject><subject>Interferon Inducers</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Poly I-C - administration & dosage</subject><subject>Poly I-C - analysis</subject><subject>Poly I-C - chemical synthesis</subject><subject>Poly I-C - toxicity</subject><subject>Polynucleotides - analysis</subject><subject>Rabbits</subject><subject>Ribonucleases - metabolism</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMoc06_gCDkC9Tl5k-z-DbKpoOBgtPX0KTJ7Nja0XZg_fSm6-ajT5d77jkXzg-heyCPDCYwJoRJJamMgEPEYsX4BRqCYKJb1CUadoaoc1yjm7reEAJC0niABjzmQgo-RJ-rL4dn3jvb4NLj6bZxVV6scRPk9_zHdeJbuW1xgsviqK7K79zmTYvTIsPTosnXruiFs3XxlNyiK59ua3d3miP0MZ-tkpdo-fq8SKbLyDI2aSKWGiEdZcxm1AIYqYzngipghhgw1vHMx95QkzpQSnnLBDglZPDYUFOyEaL9X1uVdV05r_dVvkurVgPRHSN9ZqQDI31kFEIPfWh_MDuX_UVOUMJ93N_rdO30pjxURejw38dfOyRt5A</recordid><startdate>197212</startdate><enddate>197212</enddate><creator>Lampson, George P.</creator><creator>Nemes, Marjorie M.</creator><creator>Field, A. Kirk</creator><creator>Tytell, Alfred A.</creator><creator>Hilleman, Maurice R.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>197212</creationdate><title>The Effect of Altering the Size of Poly C on the Toxicity and Antigenicity of Poly I:C</title><author>Lampson, George P. ; Nemes, Marjorie M. ; Field, A. Kirk ; Tytell, Alfred A. ; Hilleman, Maurice R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-3ab57e233cd2c11b79bf452913b0b1bce4df6fb2bae1999fc351e957452c53573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antigens</topic><topic>Biological Assay</topic><topic>Chromatography, Gel</topic><topic>Cytosine Nucleotides - analysis</topic><topic>Injections, Intraperitoneal</topic><topic>Injections, Intravenous</topic><topic>Interferon Inducers</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Poly I-C - administration & dosage</topic><topic>Poly I-C - analysis</topic><topic>Poly I-C - chemical synthesis</topic><topic>Poly I-C - toxicity</topic><topic>Polynucleotides - analysis</topic><topic>Rabbits</topic><topic>Ribonucleases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lampson, George P.</creatorcontrib><creatorcontrib>Nemes, Marjorie M.</creatorcontrib><creatorcontrib>Field, A. Kirk</creatorcontrib><creatorcontrib>Tytell, Alfred A.</creatorcontrib><creatorcontrib>Hilleman, Maurice R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lampson, George P.</au><au>Nemes, Marjorie M.</au><au>Field, A. Kirk</au><au>Tytell, Alfred A.</au><au>Hilleman, Maurice R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Altering the Size of Poly C on the Toxicity and Antigenicity of Poly I:C</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1972-12</date><risdate>1972</risdate><volume>141</volume><issue>3</issue><spage>1068</spage><epage>1072</epage><pages>1068-1072</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Appropriate reduction in size of poly C by degradation with ribonuclease prior to complexing with poly I effected marked reduction in toxicity for mice and antigenicity for rabbits of poly I:C without detectable reduction in in vivo or in vitro protection against viral infection. There was slight reduction in interferon-inducing capacity for rabbits. Such procedure adds another possible means for increasing the safety of poly I:C in eventual clinical application.
The authors are indebted to Mary-Ellen Davies, Helen Perry, Carolyn Saydah and Carol Bonoma for excellent technical assistance.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>4645754</pmid><doi>10.3181/00379727-141-36934</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1972-12, Vol.141 (3), p.1068-1072 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies - analysis Antigens Biological Assay Chromatography, Gel Cytosine Nucleotides - analysis Injections, Intraperitoneal Injections, Intravenous Interferon Inducers Mice Molecular Weight Poly I-C - administration & dosage Poly I-C - analysis Poly I-C - chemical synthesis Poly I-C - toxicity Polynucleotides - analysis Rabbits Ribonucleases - metabolism |
| title | The Effect of Altering the Size of Poly C on the Toxicity and Antigenicity of Poly I:C |
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