[48] Purification of coupling factor B

This chapter describes the coupling factor B activity as assayed by measuring the stimulation of anaerobic ATP-driven NAD reduction by succinate in ammonia ethylenediaminetetraacetic acid (AE) particles at 38°C. The particles exposed to ethylenediaminetetraacetic acid (EDTA) at pH 8.5–8.8 are select...

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Veröffentlicht in:	Methods in Enzymology 1979, Vol.55, p.384-391
Hauptverfasser:	Joshi, Saroj, Sanadi, R.Rao
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Animals Cattle Immunoassay Macromolecular Substances Methods Mitochondria, Heart - metabolism Molecular Weight Oxidative Phosphorylation Coupling Factors - isolation & purification Oxidative Phosphorylation Coupling Factors - metabolism
Online-Zugang:	Volltext
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