Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells
Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two di...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1974-07, Vol.71 (7), p.2632-2636 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Quinlan, Thomas J. Billings, Peter B. Martin, Terence E. |
| description | Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species. |
| doi_str_mv | 10.1073/pnas.71.7.2632 |
| format | Article |
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The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.71.7.2632</identifier><identifier>PMID: 4368966</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adenine Nucleotides - analysis ; Adenosine - metabolism ; Animals ; Ascites ; Ascitic Fluid - cytology ; Biological Sciences: Biochemistry ; Carcinoma, Hepatocellular - metabolism ; Cell Line ; Centrifugation, Density Gradient ; Composite particles ; Cytoplasm ; Electrophoresis, Polyacrylamide Gel ; Gels ; Heterogeneous nuclear RNA ; Liver Neoplasms - metabolism ; Messenger RNA ; Mice ; Micropore Filters ; Neoplasms, Experimental - metabolism ; Nucleoproteins - analysis ; Peptides - analysis ; Polynucleotides - analysis ; Polyribosomes ; Radioactive decay ; Ribonucleoproteins ; RNA ; RNA - analysis ; Tritium</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1974-07, Vol.71 (7), p.2632-2636</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ff97a25b2de9e3f0537a8f024cfe4b664a7b1e87a37a21c1e839844fe039269c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/71/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/63616$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/63616$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4368966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Quinlan, Thomas J.</creatorcontrib><creatorcontrib>Billings, Peter B.</creatorcontrib><creatorcontrib>Martin, Terence E.</creatorcontrib><title>Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.</description><subject>Adenine Nucleotides - analysis</subject><subject>Adenosine - metabolism</subject><subject>Animals</subject><subject>Ascites</subject><subject>Ascitic Fluid - cytology</subject><subject>Biological Sciences: Biochemistry</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Cell Line</subject><subject>Centrifugation, Density Gradient</subject><subject>Composite particles</subject><subject>Cytoplasm</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gels</subject><subject>Heterogeneous nuclear RNA</subject><subject>Liver Neoplasms - metabolism</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>Micropore Filters</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Nucleoproteins - analysis</subject><subject>Peptides - analysis</subject><subject>Polynucleotides - analysis</subject><subject>Polyribosomes</subject><subject>Radioactive decay</subject><subject>Ribonucleoproteins</subject><subject>RNA</subject><subject>RNA - analysis</subject><subject>Tritium</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2P1CAYxonRrOPq1YOJSU97a-WrQA8eNhO_kvUjxj0T2nlZu6FQgZqd_16aGSfjxRNPeH4P8PIg9JLghmDJ3szepEaSRjZUMPoIbQjuSC14hx-jDcZU1opT_hQ9S-keY9y1Cl-gC86E6oTYoNsvy-DAxOr72Ae_6jDHkGH01TZMs4MHSEX5bEY_-rvqW3B7swO_dyZDZWOYqs9hSVBdp2HMKwvOpefoiTUuwYvjeolu37_7sf1Y33z98Gl7fVMPvBW5traThrY93UEHzOKWSaMspnywwHshuJE9ASVN2adkKJJ1inMLmHVUdAO7RG8P585LP8FuAJ-jcXqO42TiXgcz6n8dP_7Ud-G3Zkq1FJf81TEfw68FUtbTmIYygfFQptKKcklbygvYHMAhhpQi2NMdBOu1B732oCXRUq89lMDr85ed8OPHn_lr7q97nr_6n6_t4lyGh1zAVwfwPuUQT6Rgggj2B4P6pyQ</recordid><startdate>19740701</startdate><enddate>19740701</enddate><creator>Quinlan, Thomas J.</creator><creator>Billings, Peter B.</creator><creator>Martin, Terence E.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19740701</creationdate><title>Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells</title><author>Quinlan, Thomas J. ; Billings, Peter B. ; Martin, Terence E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ff97a25b2de9e3f0537a8f024cfe4b664a7b1e87a37a21c1e839844fe039269c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Adenine Nucleotides - analysis</topic><topic>Adenosine - metabolism</topic><topic>Animals</topic><topic>Ascites</topic><topic>Ascitic Fluid - cytology</topic><topic>Biological Sciences: Biochemistry</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Cell Line</topic><topic>Centrifugation, Density Gradient</topic><topic>Composite particles</topic><topic>Cytoplasm</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Gels</topic><topic>Heterogeneous nuclear RNA</topic><topic>Liver Neoplasms - metabolism</topic><topic>Messenger RNA</topic><topic>Mice</topic><topic>Micropore Filters</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Nucleoproteins - analysis</topic><topic>Peptides - analysis</topic><topic>Polynucleotides - analysis</topic><topic>Polyribosomes</topic><topic>Radioactive decay</topic><topic>Ribonucleoproteins</topic><topic>RNA</topic><topic>RNA - analysis</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quinlan, Thomas J.</creatorcontrib><creatorcontrib>Billings, Peter B.</creatorcontrib><creatorcontrib>Martin, Terence E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quinlan, Thomas J.</au><au>Billings, Peter B.</au><au>Martin, Terence E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1974-07-01</date><risdate>1974</risdate><volume>71</volume><issue>7</issue><spage>2632</spage><epage>2636</epage><pages>2632-2636</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4368966</pmid><doi>10.1073/pnas.71.7.2632</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1974-07, Vol.71 (7), p.2632-2636 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenine Nucleotides - analysis Adenosine - metabolism Animals Ascites Ascitic Fluid - cytology Biological Sciences: Biochemistry Carcinoma, Hepatocellular - metabolism Cell Line Centrifugation, Density Gradient Composite particles Cytoplasm Electrophoresis, Polyacrylamide Gel Gels Heterogeneous nuclear RNA Liver Neoplasms - metabolism Messenger RNA Mice Micropore Filters Neoplasms, Experimental - metabolism Nucleoproteins - analysis Peptides - analysis Polynucleotides - analysis Polyribosomes Radioactive decay Ribonucleoproteins RNA RNA - analysis Tritium |
| title | Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells |
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