Comparative studies of normal and genetically hyperplastic lens epithelia II: Lectin-induced cell agglutination and 125I-lectin uptake by cells
The reactions of lectins with dissociated and cultured epithelial cells from hyperplastic lenses of two unrelated chick strains, Hy-1 and Hy-2, characterised by hyperplasia of the lens epithelium, and lenses of a normal genotype (N) were investigated using four different lectins. Three methods of mo...
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| Veröffentlicht in: | Current eye research 1985-03, Vol.4 (3), p.161-168 |
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| container_title | Current eye research |
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| creator | Odeigah, P. G. C. Clayton, R. M. Truman, D. E. S. |
| description | The reactions of lectins with dissociated and cultured epithelial cells from hyperplastic lenses of two unrelated chick strains, Hy-1 and Hy-2, characterised by hyperplasia of the lens epithelium, and lenses of a normal genotype (N) were investigated using four different lectins. Three methods of monitoring lectin-binding to cell surfaces were employed. Each of the four lectins used showed an individual pattern of reactivity to separated membrane components. Data obtained with the three labelling methods showed the same trend viz: increased agglutinability and high affinity for binding of all four lectins by Hy-1 and Hy-2 cells. These results suggest that Hy-1 and Hy-2 lens epithelial cells are characterised by alteration in their cell surfaces. |
| doi_str_mv | 10.3109/02713688509000845 |
| format | Article |
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These results suggest that Hy-1 and Hy-2 lens epithelial cells are characterised by alteration in their cell surfaces.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.3109/02713688509000845</identifier><identifier>PMID: 4017620</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Agglutination - drug effects ; Animals ; Animals, Newborn ; Cell Membrane - metabolism ; Chickens ; Epithelium - metabolism ; Glycopeptides - metabolism ; Hyperplasia - genetics ; Hyperplasia - metabolism ; Iodine Radioisotopes ; Isoelectric Focusing ; Lectins - metabolism ; Lectins - pharmacology ; Lens, Crystalline - metabolism ; Lens, Crystalline - pathology</subject><ispartof>Current eye research, 1985-03, Vol.4 (3), p.161-168</ispartof><rights>1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/02713688509000845$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/02713688509000845$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,59624,59730,60413,60519,61198,61233,61379,61414</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4017620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Odeigah, P. 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These results suggest that Hy-1 and Hy-2 lens epithelial cells are characterised by alteration in their cell surfaces.</description><subject>Agglutination - drug effects</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Cell Membrane - metabolism</subject><subject>Chickens</subject><subject>Epithelium - metabolism</subject><subject>Glycopeptides - metabolism</subject><subject>Hyperplasia - genetics</subject><subject>Hyperplasia - metabolism</subject><subject>Iodine Radioisotopes</subject><subject>Isoelectric Focusing</subject><subject>Lectins - metabolism</subject><subject>Lectins - pharmacology</subject><subject>Lens, Crystalline - metabolism</subject><subject>Lens, Crystalline - pathology</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc2OFCEUhYnRjO3oA7gwYeWuFCiqqlvdmI4_nXQym5k14efSzUhBCZSmnsJXHrqnN8a4ICT3fOdAzkXoNSXvWko27wkbaNuv1x3ZEELWvHuCVpT3pGGMsKdoddKbCrTP0Yuc7ytTB_wKXXFCh56RFfqzjeMkkyzuF-BcZuMg42hxiGmUHstg8AECFKel9ws-LhOkyctcB9hDyBgmV47gncS73Qe8B11caFwwswaDNfiacTj4uU7rGzGcEynrdo0_o3ieivwBWC1nOL9Ez6z0GV5d7mt09_XL7fZ7s7_5ttt-3jeOdmvS8I4r3XEKqlecc6JsL2lnWW8HxXtljAFugFmijZJEK9YNhveGtMway9jQXqO3j7lTij9nyEWMLp9-IAPEOYvaDuUt3VTwzQWc1QhGTMmNMi3i0mDVPz3qLthTZ79j8kYUufiYbJJBuyzqrk5nI_7ZV7V__Mt-BOnLUcsE4j7OKdQOxP_dD6avmyw</recordid><startdate>198503</startdate><enddate>198503</enddate><creator>Odeigah, P. 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M.</creatorcontrib><creatorcontrib>Truman, D. E. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Odeigah, P. G. C.</au><au>Clayton, R. M.</au><au>Truman, D. E. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative studies of normal and genetically hyperplastic lens epithelia II: Lectin-induced cell agglutination and 125I-lectin uptake by cells</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>1985-03</date><risdate>1985</risdate><volume>4</volume><issue>3</issue><spage>161</spage><epage>168</epage><pages>161-168</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><abstract>The reactions of lectins with dissociated and cultured epithelial cells from hyperplastic lenses of two unrelated chick strains, Hy-1 and Hy-2, characterised by hyperplasia of the lens epithelium, and lenses of a normal genotype (N) were investigated using four different lectins. Three methods of monitoring lectin-binding to cell surfaces were employed. Each of the four lectins used showed an individual pattern of reactivity to separated membrane components. Data obtained with the three labelling methods showed the same trend viz: increased agglutinability and high affinity for binding of all four lectins by Hy-1 and Hy-2 cells. These results suggest that Hy-1 and Hy-2 lens epithelial cells are characterised by alteration in their cell surfaces.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>4017620</pmid><doi>10.3109/02713688509000845</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0271-3683 |
| ispartof | Current eye research, 1985-03, Vol.4 (3), p.161-168 |
| issn | 0271-3683 1460-2202 |
| language | eng |
| recordid | cdi_pubmed_primary_4017620 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Agglutination - drug effects Animals Animals, Newborn Cell Membrane - metabolism Chickens Epithelium - metabolism Glycopeptides - metabolism Hyperplasia - genetics Hyperplasia - metabolism Iodine Radioisotopes Isoelectric Focusing Lectins - metabolism Lectins - pharmacology Lens, Crystalline - metabolism Lens, Crystalline - pathology |
| title | Comparative studies of normal and genetically hyperplastic lens epithelia II: Lectin-induced cell agglutination and 125I-lectin uptake by cells |
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