Renal Transport of Gossypol in the Rabbit
Abstract The present work was carried out to investigate the transport characteristics of gossypol, a toxic weak organic acid (pK = 7.2) contained in cottonseed, into the rabbit renal cortical slice. The uptake of gossypol increased linearly during a 2-hr incubation after which it leveled off with t...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1985-05, Vol.179 (1), p.50-54 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
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| creator | Goldinger, James M. Lee, Sang Ho Hong, Suk Ki |
| description | Abstract
The present work was carried out to investigate the transport characteristics of gossypol, a toxic weak organic acid (pK = 7.2) contained in cottonseed, into the rabbit renal cortical slice. The uptake of gossypol increased linearly during a 2-hr incubation after which it leveled off with the average slice-to-medium concentration ratio (S/M) slightly above 20. In the presence of metabolic inhibitors, the S/M gossypol leveled off at about 9, suggesting an extensive binding of gossypol to tissue proteins. The uptake of gossypol was significantly inhibited by p-aminohippurate (PAH), probenecid, ouabain, and DIDS, all of which are known inhibitors of renal organic anion transport. However, the gossypol uptake was not affected by tetraethylammonium (TEA), a prototypical organic cation. Kinetic studies indicated that the apparent Km
for gossypol transport is 0.28 mM, and also that probenecid inhibits gossypol transport in a competitive manner. It is concluded that gossypol is transported by the renal tubule through the classic orgenic anion system. |
| doi_str_mv | 10.3181/00379727-179-42063 |
| format | Article |
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The present work was carried out to investigate the transport characteristics of gossypol, a toxic weak organic acid (pK = 7.2) contained in cottonseed, into the rabbit renal cortical slice. The uptake of gossypol increased linearly during a 2-hr incubation after which it leveled off with the average slice-to-medium concentration ratio (S/M) slightly above 20. In the presence of metabolic inhibitors, the S/M gossypol leveled off at about 9, suggesting an extensive binding of gossypol to tissue proteins. The uptake of gossypol was significantly inhibited by p-aminohippurate (PAH), probenecid, ouabain, and DIDS, all of which are known inhibitors of renal organic anion transport. However, the gossypol uptake was not affected by tetraethylammonium (TEA), a prototypical organic cation. Kinetic studies indicated that the apparent Km
for gossypol transport is 0.28 mM, and also that probenecid inhibits gossypol transport in a competitive manner. It is concluded that gossypol is transported by the renal tubule through the classic orgenic anion system.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-179-42063</identifier><identifier>PMID: 3991597</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>2,4-Dinitrophenol ; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid ; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives ; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology ; Animals ; Binding, Competitive ; Biological and medical sciences ; Biological Transport - drug effects ; Biological Transport, Active - drug effects ; Birth control ; Dinitrophenols - pharmacology ; Gossypol - metabolism ; Gynecology. Andrology. Obstetrics ; Hormonal contraception ; In Vitro Techniques ; Iodoacetamide - pharmacology ; Kidney Cortex - metabolism ; Kinetics ; Medical sciences ; Nitrogen - pharmacology ; Ouabain - pharmacology ; p-Aminohippuric Acid - pharmacology ; Probenecid - pharmacology ; Rabbits ; Tetraethylammonium ; Tetraethylammonium Compounds - pharmacology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1985-05, Vol.179 (1), p.50-54</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-d02f27c48f9db406435d33674d5e123a0a19a2e515d49c1f27678e3b4d6045293</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7931782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3991597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goldinger, James M.</creatorcontrib><creatorcontrib>Lee, Sang Ho</creatorcontrib><creatorcontrib>Hong, Suk Ki</creatorcontrib><title>Renal Transport of Gossypol in the Rabbit</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
The present work was carried out to investigate the transport characteristics of gossypol, a toxic weak organic acid (pK = 7.2) contained in cottonseed, into the rabbit renal cortical slice. The uptake of gossypol increased linearly during a 2-hr incubation after which it leveled off with the average slice-to-medium concentration ratio (S/M) slightly above 20. In the presence of metabolic inhibitors, the S/M gossypol leveled off at about 9, suggesting an extensive binding of gossypol to tissue proteins. The uptake of gossypol was significantly inhibited by p-aminohippurate (PAH), probenecid, ouabain, and DIDS, all of which are known inhibitors of renal organic anion transport. However, the gossypol uptake was not affected by tetraethylammonium (TEA), a prototypical organic cation. Kinetic studies indicated that the apparent Km
for gossypol transport is 0.28 mM, and also that probenecid inhibits gossypol transport in a competitive manner. It is concluded that gossypol is transported by the renal tubule through the classic orgenic anion system.</description><subject>2,4-Dinitrophenol</subject><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</subject><subject>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives</subject><subject>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology</subject><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Biological Transport, Active - drug effects</subject><subject>Birth control</subject><subject>Dinitrophenols - pharmacology</subject><subject>Gossypol - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hormonal contraception</subject><subject>In Vitro Techniques</subject><subject>Iodoacetamide - pharmacology</subject><subject>Kidney Cortex - metabolism</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Nitrogen - pharmacology</subject><subject>Ouabain - pharmacology</subject><subject>p-Aminohippuric Acid - pharmacology</subject><subject>Probenecid - pharmacology</subject><subject>Rabbits</subject><subject>Tetraethylammonium</subject><subject>Tetraethylammonium Compounds - pharmacology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OwzAURi0EKqHwAkhIGVgYTH39G4-oKgWpElJVZsuJHUiVNpGdDn17XFo6Mnn4znevz0XoHsgzgwImhDClFVUYlMacEskuUAaCCcyk1pcoOwD4QFyjmxjXhIBQVI7QiGkNQqsMPS391rb5Ktht7Lsw5F2dz7sY933X5s02H759vrRl2Qy36Kq2bfR3p3eMPl9nq-kbXnzM36cvC1wxqQbsCK2pqnhRa1dyIjkTjqWEO-GBMkssaEu9AOG4riCxUhWeldxJwgXVbIzocW4V0j-Cr00fmo0NewPEHLTNn7ZJ2uZXO5UejqV-V268O1dOnil_POU2Vratk27VxDOmNANV0IRNjli0X96su11Ix4n_Lf4B835qdA</recordid><startdate>198505</startdate><enddate>198505</enddate><creator>Goldinger, James M.</creator><creator>Lee, Sang Ho</creator><creator>Hong, Suk Ki</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198505</creationdate><title>Renal Transport of Gossypol in the Rabbit</title><author>Goldinger, James M. ; Lee, Sang Ho ; Hong, Suk Ki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-d02f27c48f9db406435d33674d5e123a0a19a2e515d49c1f27678e3b4d6045293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>2,4-Dinitrophenol</topic><topic>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</topic><topic>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives</topic><topic>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology</topic><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Biological Transport, Active - drug effects</topic><topic>Birth control</topic><topic>Dinitrophenols - pharmacology</topic><topic>Gossypol - metabolism</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Hormonal contraception</topic><topic>In Vitro Techniques</topic><topic>Iodoacetamide - pharmacology</topic><topic>Kidney Cortex - metabolism</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Nitrogen - pharmacology</topic><topic>Ouabain - pharmacology</topic><topic>p-Aminohippuric Acid - pharmacology</topic><topic>Probenecid - pharmacology</topic><topic>Rabbits</topic><topic>Tetraethylammonium</topic><topic>Tetraethylammonium Compounds - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goldinger, James M.</creatorcontrib><creatorcontrib>Lee, Sang Ho</creatorcontrib><creatorcontrib>Hong, Suk Ki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goldinger, James M.</au><au>Lee, Sang Ho</au><au>Hong, Suk Ki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Renal Transport of Gossypol in the Rabbit</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1985-05</date><risdate>1985</risdate><volume>179</volume><issue>1</issue><spage>50</spage><epage>54</epage><pages>50-54</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
The present work was carried out to investigate the transport characteristics of gossypol, a toxic weak organic acid (pK = 7.2) contained in cottonseed, into the rabbit renal cortical slice. The uptake of gossypol increased linearly during a 2-hr incubation after which it leveled off with the average slice-to-medium concentration ratio (S/M) slightly above 20. In the presence of metabolic inhibitors, the S/M gossypol leveled off at about 9, suggesting an extensive binding of gossypol to tissue proteins. The uptake of gossypol was significantly inhibited by p-aminohippurate (PAH), probenecid, ouabain, and DIDS, all of which are known inhibitors of renal organic anion transport. However, the gossypol uptake was not affected by tetraethylammonium (TEA), a prototypical organic cation. Kinetic studies indicated that the apparent Km
for gossypol transport is 0.28 mM, and also that probenecid inhibits gossypol transport in a competitive manner. It is concluded that gossypol is transported by the renal tubule through the classic orgenic anion system.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>3991597</pmid><doi>10.3181/00379727-179-42063</doi><tpages>5</tpages></addata></record> |
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| ispartof | Experimental biology and medicine (Maywood, N.J.), 1985-05, Vol.179 (1), p.50-54 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 2,4-Dinitrophenol 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology Animals Binding, Competitive Biological and medical sciences Biological Transport - drug effects Biological Transport, Active - drug effects Birth control Dinitrophenols - pharmacology Gossypol - metabolism Gynecology. Andrology. Obstetrics Hormonal contraception In Vitro Techniques Iodoacetamide - pharmacology Kidney Cortex - metabolism Kinetics Medical sciences Nitrogen - pharmacology Ouabain - pharmacology p-Aminohippuric Acid - pharmacology Probenecid - pharmacology Rabbits Tetraethylammonium Tetraethylammonium Compounds - pharmacology |
| title | Renal Transport of Gossypol in the Rabbit |
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