Induction of forward mutation at the yg2 locus in maize by ethylnitrosourea
We analyzed the kinetics of mutation induction at the yg2 locus in Zea mays by N‐ethyl‐N‐nitrosourea (ENU). ENU concentrations ranged from O.l to 10.7 mM and a linear, concentration‐dependent response was observed for the induction of yg2 sectors in leaf 4 and leaf 5. For both leaf 4 and leaf 5 ENU...
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| Veröffentlicht in: | Environ. Mutagen.; (United States) 1985, Vol.7 (2), p.155-162 |
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| creator | Schy, William E. Plewa, Michael J. |
| description | We analyzed the kinetics of mutation induction at the yg2 locus in Zea mays by N‐ethyl‐N‐nitrosourea (ENU). ENU concentrations ranged from O.l to 10.7 mM and a linear, concentration‐dependent response was observed for the induction of yg2 sectors in leaf 4 and leaf 5. For both leaf 4 and leaf 5 ENU exhibited one‐hit kinetics consistent with either a point‐mutational or chromosome‐breakage mechanism of mutation induction. The mean induced mutation rate/mM ENU for the combined leaf 4 and leaf 5 data was 6.77 × 10−4 mutations/locus/mM ENU. This value was used to compare the mutagenic effectiveness of ENU with ethyl methanesulfonate (EMS) and to calculate a rad equivalent for ENU. ENU was found to be 3.8‐fold more effective than EMS. Based on their respective chemical reactivities with DNA, we propose that ENU predominantly induces point mutations at the yg2 locus. One rad of gamma radiation induced the same rate of mutation as an 8‐hr exposure to a 6.71 × 10−6 M concentration of ENU at 20°C. |
| doi_str_mv | 10.1002/em.2860070204 |
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ENU concentrations ranged from O.l to 10.7 mM and a linear, concentration‐dependent response was observed for the induction of yg2 sectors in leaf 4 and leaf 5. For both leaf 4 and leaf 5 ENU exhibited one‐hit kinetics consistent with either a point‐mutational or chromosome‐breakage mechanism of mutation induction. The mean induced mutation rate/mM ENU for the combined leaf 4 and leaf 5 data was 6.77 × 10−4 mutations/locus/mM ENU. This value was used to compare the mutagenic effectiveness of ENU with ethyl methanesulfonate (EMS) and to calculate a rad equivalent for ENU. ENU was found to be 3.8‐fold more effective than EMS. Based on their respective chemical reactivities with DNA, we propose that ENU predominantly induces point mutations at the yg2 locus. One rad of gamma radiation induced the same rate of mutation as an 8‐hr exposure to a 6.71 × 10−6 M concentration of ENU at 20°C.</description><identifier>ISSN: 0192-2521</identifier><identifier>EISSN: 1930-238X</identifier><identifier>DOI: 10.1002/em.2860070204</identifier><identifier>PMID: 3971955</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; CEREALS ; Chemical mutagenesis ; Dose-Response Relationship, Drug ; ELECTROMAGNETIC RADIATION ; Ethyl Methanesulfonate - toxicity ; ethylnitrosourea ; Ethylnitrosourea - toxicity ; forward mutation ; GAMMA RADIATION ; GENE MUTATIONS ; GENETIC EFFECTS ; GRASS ; IONIZING RADIATIONS ; MAIZE ; Medical sciences ; mutagenic effectiveness ; Mutation ; MUTATION FREQUENCY ; MUTATIONS ; NITROSO COMPOUNDS ; Nitrosourea Compounds - toxicity ; NITROSOUREAS ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; PLANTS ; rad equivalent ; RADIATION EQUIVALENCE ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; Toxicology ; yg2 ; Zea mays ; Zea mays - genetics</subject><ispartof>Environ. Mutagen.; (United States), 1985, Vol.7 (2), p.155-162</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1985 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fem.2860070204$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fem.2860070204$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,881,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9214007$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3971955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5662680$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Schy, William E.</creatorcontrib><creatorcontrib>Plewa, Michael J.</creatorcontrib><creatorcontrib>Univ. of Illinois, Urbana</creatorcontrib><title>Induction of forward mutation at the yg2 locus in maize by ethylnitrosourea</title><title>Environ. Mutagen.; (United States)</title><addtitle>Environ. mutagen</addtitle><description>We analyzed the kinetics of mutation induction at the yg2 locus in Zea mays by N‐ethyl‐N‐nitrosourea (ENU). ENU concentrations ranged from O.l to 10.7 mM and a linear, concentration‐dependent response was observed for the induction of yg2 sectors in leaf 4 and leaf 5. For both leaf 4 and leaf 5 ENU exhibited one‐hit kinetics consistent with either a point‐mutational or chromosome‐breakage mechanism of mutation induction. The mean induced mutation rate/mM ENU for the combined leaf 4 and leaf 5 data was 6.77 × 10−4 mutations/locus/mM ENU. This value was used to compare the mutagenic effectiveness of ENU with ethyl methanesulfonate (EMS) and to calculate a rad equivalent for ENU. ENU was found to be 3.8‐fold more effective than EMS. Based on their respective chemical reactivities with DNA, we propose that ENU predominantly induces point mutations at the yg2 locus. One rad of gamma radiation induced the same rate of mutation as an 8‐hr exposure to a 6.71 × 10−6 M concentration of ENU at 20°C.</description><subject>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>CEREALS</subject><subject>Chemical mutagenesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Ethyl Methanesulfonate - toxicity</subject><subject>ethylnitrosourea</subject><subject>Ethylnitrosourea - toxicity</subject><subject>forward mutation</subject><subject>GAMMA RADIATION</subject><subject>GENE MUTATIONS</subject><subject>GENETIC EFFECTS</subject><subject>GRASS</subject><subject>IONIZING RADIATIONS</subject><subject>MAIZE</subject><subject>Medical sciences</subject><subject>mutagenic effectiveness</subject><subject>Mutation</subject><subject>MUTATION FREQUENCY</subject><subject>MUTATIONS</subject><subject>NITROSO COMPOUNDS</subject><subject>Nitrosourea Compounds - toxicity</subject><subject>NITROSOUREAS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>PLANTS</subject><subject>rad equivalent</subject><subject>RADIATION EQUIVALENCE</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>Toxicology</subject><subject>yg2</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><issn>0192-2521</issn><issn>1930-238X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1v1DAQhi0EKkvhyBHJQlxTxuOv5IiqUgpbkBCovVmOM2EN-ahiRyX8egK72p6s8fO8I83L2EsBZwIA31J_hqUBsICgHrGNqCQUKMvbx2wDosICNYqn7FlKPwG0QlOdsBNZWVFpvWGfroZmDjmOAx9b3o7TvZ8a3s_Z___zmecd8eUH8m4Mc-Jx4L2Pf4jXC6e8W7oh5mlM4zyRf86etL5L9OLwnrLv7y--nX8otl8ur87fbYsoVakKVHVjTWhFWYI3Aqi1NRkgoZRC4RtBVCOhxNLqylhhq3qVwQdJKwItT9nr_d4x5ehSiJnCLozDQCE7bQyaElbp1V66m-ueGnc3xd5PiztcvvI3B-5T8F07-SHEdNQqFGrtdNXsXruPHS1HLMD9K99R7x7KdxfXD8OaLPbJmDL9Pib99MsZK612N58v3TXeavvxZuu-yr8V3YWZ</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Schy, William E.</creator><creator>Plewa, Michael J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>OTOTI</scope></search><sort><creationdate>1985</creationdate><title>Induction of forward mutation at the yg2 locus in maize by ethylnitrosourea</title><author>Schy, William E. ; Plewa, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3484-24bd76cf1880a610ef7be60e144421ad1eeb2e232875967179bcf10ac3e1ee053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>CEREALS</topic><topic>Chemical mutagenesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Ethyl Methanesulfonate - toxicity</topic><topic>ethylnitrosourea</topic><topic>Ethylnitrosourea - toxicity</topic><topic>forward mutation</topic><topic>GAMMA RADIATION</topic><topic>GENE MUTATIONS</topic><topic>GENETIC EFFECTS</topic><topic>GRASS</topic><topic>IONIZING RADIATIONS</topic><topic>MAIZE</topic><topic>Medical sciences</topic><topic>mutagenic effectiveness</topic><topic>Mutation</topic><topic>MUTATION FREQUENCY</topic><topic>MUTATIONS</topic><topic>NITROSO COMPOUNDS</topic><topic>Nitrosourea Compounds - toxicity</topic><topic>NITROSOUREAS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>PLANTS</topic><topic>rad equivalent</topic><topic>RADIATION EQUIVALENCE</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>Toxicology</topic><topic>yg2</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Schy, William E.</creatorcontrib><creatorcontrib>Plewa, Michael J.</creatorcontrib><creatorcontrib>Univ. of Illinois, Urbana</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>OSTI.GOV</collection><jtitle>Environ. Mutagen.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schy, William E.</au><au>Plewa, Michael J.</au><aucorp>Univ. of Illinois, Urbana</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of forward mutation at the yg2 locus in maize by ethylnitrosourea</atitle><jtitle>Environ. Mutagen.; (United States)</jtitle><addtitle>Environ. mutagen</addtitle><date>1985</date><risdate>1985</risdate><volume>7</volume><issue>2</issue><spage>155</spage><epage>162</epage><pages>155-162</pages><issn>0192-2521</issn><eissn>1930-238X</eissn><abstract>We analyzed the kinetics of mutation induction at the yg2 locus in Zea mays by N‐ethyl‐N‐nitrosourea (ENU). ENU concentrations ranged from O.l to 10.7 mM and a linear, concentration‐dependent response was observed for the induction of yg2 sectors in leaf 4 and leaf 5. For both leaf 4 and leaf 5 ENU exhibited one‐hit kinetics consistent with either a point‐mutational or chromosome‐breakage mechanism of mutation induction. The mean induced mutation rate/mM ENU for the combined leaf 4 and leaf 5 data was 6.77 × 10−4 mutations/locus/mM ENU. This value was used to compare the mutagenic effectiveness of ENU with ethyl methanesulfonate (EMS) and to calculate a rad equivalent for ENU. ENU was found to be 3.8‐fold more effective than EMS. Based on their respective chemical reactivities with DNA, we propose that ENU predominantly induces point mutations at the yg2 locus. One rad of gamma radiation induced the same rate of mutation as an 8‐hr exposure to a 6.71 × 10−6 M concentration of ENU at 20°C.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3971955</pmid><doi>10.1002/em.2860070204</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Environ. Mutagen.; (United States), 1985, Vol.7 (2), p.155-162 |
| issn | 0192-2521 1930-238X |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) Biological and medical sciences BIOLOGICAL EFFECTS CEREALS Chemical mutagenesis Dose-Response Relationship, Drug ELECTROMAGNETIC RADIATION Ethyl Methanesulfonate - toxicity ethylnitrosourea Ethylnitrosourea - toxicity forward mutation GAMMA RADIATION GENE MUTATIONS GENETIC EFFECTS GRASS IONIZING RADIATIONS MAIZE Medical sciences mutagenic effectiveness Mutation MUTATION FREQUENCY MUTATIONS NITROSO COMPOUNDS Nitrosourea Compounds - toxicity NITROSOUREAS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PLANTS rad equivalent RADIATION EQUIVALENCE RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS Toxicology yg2 Zea mays Zea mays - genetics |
| title | Induction of forward mutation at the yg2 locus in maize by ethylnitrosourea |
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