Protocol for single-molecule FISH in the developing mouse retinal vasculature

The developing vasculature of the post-natal mouse retina is a powerful model to discover mechanisms of vessel formation and to test modulators of neovascularization. We present a protocol for single-molecule fluorescent in situ hybridization (smFISH) in whole-mount mouse retinas enabling the detect...

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Veröffentlicht in:	STAR protocols 2024-12, Vol.5 (4), p.103510, Article 103510
Hauptverfasser:	Augustine, Josy, Smith, Madeleine R., Delaney, Ryan, Owuamalam, Precious O., Costa, Guilherme
Format:	Artikel
Sprache:	eng
Schlagworte:	developmental biology gene expression in situ hybridization
Online-Zugang:	Volltext
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