Dynamic behavior and lineage plasticity of the pulmonary venous endothelium

Repair of the pulmonary vascular bed and the origin of new vasculature remain underexplored despite the critical necessity to meet oxygen demands after injury. Given their critical role in angiogenesis in other settings, we investigated the role of venous endothelial cells in endothelial regeneratio...

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Veröffentlicht in:Nature Cardiovascular Research 2024-12, Vol.3 (12), p.1584-1600
Hauptverfasser: Wong, Joanna, Zhao, Gan, Adams-Tzivelekidis, Stephanie, Wen, Hongbo, Chandrasekaran, Prashant, Michki, Sylvia N., Gentile, Maria E., Singh, Madeline, Kass-Gergi, Sara, Mendoza, Meryl, Holcomb, Nicolas P., Li, Xinyuan, Tang, Alan T., Negretti, Nicholas M., Sucre, Jennifer M. S., Frank, David B., Vaughan, Andrew E.
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container_end_page 1600
container_issue 12
container_start_page 1584
container_title Nature Cardiovascular Research
container_volume 3
creator Wong, Joanna
Zhao, Gan
Adams-Tzivelekidis, Stephanie
Wen, Hongbo
Chandrasekaran, Prashant
Michki, Sylvia N.
Gentile, Maria E.
Singh, Madeline
Kass-Gergi, Sara
Mendoza, Meryl
Holcomb, Nicolas P.
Li, Xinyuan
Tang, Alan T.
Negretti, Nicholas M.
Sucre, Jennifer M. S.
Frank, David B.
Vaughan, Andrew E.
description Repair of the pulmonary vascular bed and the origin of new vasculature remain underexplored despite the critical necessity to meet oxygen demands after injury. Given their critical role in angiogenesis in other settings, we investigated the role of venous endothelial cells in endothelial regeneration after adult lung injury. Here we identified Slc6a2 as a marker of pulmonary venous endothelial cells and generated a venous-specific, inducible Cre mouse line. We observed that venous endothelial cells proliferate into the adjacent capillary bed upon influenza injury and hyperoxia injury. Imaging analysis demonstrated that venous endothelial cells proliferate and differentiate into general capillary and aerocyte capillary endothelial cells after infection, thus contributing to repair of the capillary plexus vital for gas exchange. Our studies thus establish that venous endothelial cells exhibit demonstrable progenitor capacity upon respiratory viral injury and sterile injury, contributing to repair of the alveolar capillary bed responsible for pulmonary function. Wong et al. identify Slc6a2 as a marker of pulmonary venous endothelial cells and demonstrate that these cells differentiate into capillary endothelial cells during vascular regeneration after lung injury.
doi_str_mv 10.1038/s44161-024-00573-2
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subjects 14
14/19
45
45/91
631/136/16/1986
631/532/489
Animals
Biomedical and Life Sciences
Capillaries - physiology
Cardiovascular Biology
Cell Differentiation
Cell Lineage
Cell Plasticity - physiology
Cell Proliferation
Disease Models, Animal
Endothelial Cells - physiology
Endothelial Progenitor Cells - metabolism
Endothelial Progenitor Cells - physiology
Endothelium, Vascular - cytology
Hyperoxia - physiopathology
Life Sciences
Lung - blood supply
Lung Injury - pathology
Lung Injury - physiopathology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neovascularization, Physiologic - physiology
Orthomyxoviridae Infections - virology
Pulmonary Veins
Regeneration - physiology
technical-report
title Dynamic behavior and lineage plasticity of the pulmonary venous endothelium
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