Rhododendron luteum Sweet Flower Supercritical CO 2 Extracts: Terpenes Composition, Pro-Inflammatory Enzymes Inhibition and Antioxidant Activity
Terpenes are plant secondary metabolites known for their anti-inflammatory and antioxidant activities. According to ethnobotanical knowledge, Sweet was used in traditional medicine against inflammation. The present study was conducted to determine the triterpene profile and antioxidant and anti-infl...
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| Veröffentlicht in: | International journal of molecular sciences 2024-09, Vol.25 (18) |
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| creator | Łyko, Lena Olech, Marta Gawlik, Urszula Krajewska, Agnieszka Kalemba, Danuta Tyśkiewicz, Katarzyna Piórecki, Narcyz Prokopiv, Andriy Nowak, Renata |
| description | Terpenes are plant secondary metabolites known for their anti-inflammatory and antioxidant activities. According to ethnobotanical knowledge,
Sweet was used in traditional medicine against inflammation. The present study was conducted to determine the triterpene profile and antioxidant and anti-inflammatory activity of supercritical CO
(SC-CO
) extracts of
Sweet flower (RLF). An LC-APCI-MS/MS analysis showed the presence of eight pentacyclic triterpenes and one phytosterol in the extracts obtained with pure CO
as well as CO
with the addition of aqueous ethanol as a co-solvent. Among the compounds detected, oleanolic/ursolic acid, β-sitosterol and 3
-taraxerol were the most abundant. The extract obtained with pure SC-CO
was additionally subjected to HS-SPME-GC-FID-MS, which revealed more than 100 volatiles, mainly eugenol,
-phenylethanol, dodecane,
-caryophyllene, estragole and (
)- and (
)-cinnamyl alcohol, followed by
-cadinene. The extracts demonstrated significant hyaluronidase inhibition and exhibited varying modes of lipoxygenase and xanthine oxidase inhibitory activities. The studies of RLF have shown that their SC-CO
extracts can be a rich source of triterpenes with anti-inflammatory potential. |
| doi_str_mv | 10.3390/ijms25189952 |
| format | Article |
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Sweet was used in traditional medicine against inflammation. The present study was conducted to determine the triterpene profile and antioxidant and anti-inflammatory activity of supercritical CO
(SC-CO
) extracts of
Sweet flower (RLF). An LC-APCI-MS/MS analysis showed the presence of eight pentacyclic triterpenes and one phytosterol in the extracts obtained with pure CO
as well as CO
with the addition of aqueous ethanol as a co-solvent. Among the compounds detected, oleanolic/ursolic acid, β-sitosterol and 3
-taraxerol were the most abundant. The extract obtained with pure SC-CO
was additionally subjected to HS-SPME-GC-FID-MS, which revealed more than 100 volatiles, mainly eugenol,
-phenylethanol, dodecane,
-caryophyllene, estragole and (
)- and (
)-cinnamyl alcohol, followed by
-cadinene. The extracts demonstrated significant hyaluronidase inhibition and exhibited varying modes of lipoxygenase and xanthine oxidase inhibitory activities. The studies of RLF have shown that their SC-CO
extracts can be a rich source of triterpenes with anti-inflammatory potential.</description><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25189952</identifier><identifier>PMID: 39337440</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - pharmacology ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Carbon Dioxide - chemistry ; Chromatography, Supercritical Fluid - methods ; Flowers - chemistry ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Rhododendron - chemistry ; Tandem Mass Spectrometry ; Terpenes - chemistry ; Terpenes - pharmacology</subject><ispartof>International journal of molecular sciences, 2024-09, Vol.25 (18)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-4952-3147 ; 0000-0003-1690-4090 ; 0000-0002-2838-550X ; 0000-0002-4018-5260 ; 0000-0002-0010-6089 ; 0000-0002-7308-4335</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39337440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Łyko, Lena</creatorcontrib><creatorcontrib>Olech, Marta</creatorcontrib><creatorcontrib>Gawlik, Urszula</creatorcontrib><creatorcontrib>Krajewska, Agnieszka</creatorcontrib><creatorcontrib>Kalemba, Danuta</creatorcontrib><creatorcontrib>Tyśkiewicz, Katarzyna</creatorcontrib><creatorcontrib>Piórecki, Narcyz</creatorcontrib><creatorcontrib>Prokopiv, Andriy</creatorcontrib><creatorcontrib>Nowak, Renata</creatorcontrib><title>Rhododendron luteum Sweet Flower Supercritical CO 2 Extracts: Terpenes Composition, Pro-Inflammatory Enzymes Inhibition and Antioxidant Activity</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Terpenes are plant secondary metabolites known for their anti-inflammatory and antioxidant activities. According to ethnobotanical knowledge,
Sweet was used in traditional medicine against inflammation. The present study was conducted to determine the triterpene profile and antioxidant and anti-inflammatory activity of supercritical CO
(SC-CO
) extracts of
Sweet flower (RLF). An LC-APCI-MS/MS analysis showed the presence of eight pentacyclic triterpenes and one phytosterol in the extracts obtained with pure CO
as well as CO
with the addition of aqueous ethanol as a co-solvent. Among the compounds detected, oleanolic/ursolic acid, β-sitosterol and 3
-taraxerol were the most abundant. The extract obtained with pure SC-CO
was additionally subjected to HS-SPME-GC-FID-MS, which revealed more than 100 volatiles, mainly eugenol,
-phenylethanol, dodecane,
-caryophyllene, estragole and (
)- and (
)-cinnamyl alcohol, followed by
-cadinene. The extracts demonstrated significant hyaluronidase inhibition and exhibited varying modes of lipoxygenase and xanthine oxidase inhibitory activities. The studies of RLF have shown that their SC-CO
extracts can be a rich source of triterpenes with anti-inflammatory potential.</description><subject>Anti-Inflammatory Agents - chemistry</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Carbon Dioxide - chemistry</subject><subject>Chromatography, Supercritical Fluid - methods</subject><subject>Flowers - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Rhododendron - chemistry</subject><subject>Tandem Mass Spectrometry</subject><subject>Terpenes - chemistry</subject><subject>Terpenes - pharmacology</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFz71OwzAYhWELCdFS2JjRdwGEOnZCMVsVpaITiHav3Pir6so_ke3QhqvgkhshmJnOOzzLIeQup4-cCzrVBxtZmT8LUbILMs4LxjJKn2Yjch3jgVLGWSmuyIgLzmdFQcfk-2PvlVfoVPAOTJews7A6IiZYGH_EAKuuxdAEnXQjDVRvwKA-pSCbFF9gjaFFhxEqb1sfB-TdA7wHny3dzkhrZfKhh9p99XZQS7fX2x8E0imYuyFPWkmXYN4k_alTf0Mud9JEvP3dCblf1OvqNWu7rUW1aYO2MvSbvwv8X3AGHD5ZjA</recordid><startdate>20240915</startdate><enddate>20240915</enddate><creator>Łyko, Lena</creator><creator>Olech, Marta</creator><creator>Gawlik, Urszula</creator><creator>Krajewska, Agnieszka</creator><creator>Kalemba, Danuta</creator><creator>Tyśkiewicz, Katarzyna</creator><creator>Piórecki, Narcyz</creator><creator>Prokopiv, Andriy</creator><creator>Nowak, Renata</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0003-4952-3147</orcidid><orcidid>https://orcid.org/0000-0003-1690-4090</orcidid><orcidid>https://orcid.org/0000-0002-2838-550X</orcidid><orcidid>https://orcid.org/0000-0002-4018-5260</orcidid><orcidid>https://orcid.org/0000-0002-0010-6089</orcidid><orcidid>https://orcid.org/0000-0002-7308-4335</orcidid></search><sort><creationdate>20240915</creationdate><title>Rhododendron luteum Sweet Flower Supercritical CO 2 Extracts: Terpenes Composition, Pro-Inflammatory Enzymes Inhibition and Antioxidant Activity</title><author>Łyko, Lena ; Olech, Marta ; Gawlik, Urszula ; Krajewska, Agnieszka ; Kalemba, Danuta ; Tyśkiewicz, Katarzyna ; Piórecki, Narcyz ; Prokopiv, Andriy ; Nowak, Renata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_393374403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anti-Inflammatory Agents - chemistry</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>Carbon Dioxide - chemistry</topic><topic>Chromatography, Supercritical Fluid - methods</topic><topic>Flowers - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Rhododendron - chemistry</topic><topic>Tandem Mass Spectrometry</topic><topic>Terpenes - chemistry</topic><topic>Terpenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Łyko, Lena</creatorcontrib><creatorcontrib>Olech, Marta</creatorcontrib><creatorcontrib>Gawlik, Urszula</creatorcontrib><creatorcontrib>Krajewska, Agnieszka</creatorcontrib><creatorcontrib>Kalemba, Danuta</creatorcontrib><creatorcontrib>Tyśkiewicz, Katarzyna</creatorcontrib><creatorcontrib>Piórecki, Narcyz</creatorcontrib><creatorcontrib>Prokopiv, Andriy</creatorcontrib><creatorcontrib>Nowak, Renata</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Łyko, Lena</au><au>Olech, Marta</au><au>Gawlik, Urszula</au><au>Krajewska, Agnieszka</au><au>Kalemba, Danuta</au><au>Tyśkiewicz, Katarzyna</au><au>Piórecki, Narcyz</au><au>Prokopiv, Andriy</au><au>Nowak, Renata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhododendron luteum Sweet Flower Supercritical CO 2 Extracts: Terpenes Composition, Pro-Inflammatory Enzymes Inhibition and Antioxidant Activity</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2024-09-15</date><risdate>2024</risdate><volume>25</volume><issue>18</issue><eissn>1422-0067</eissn><abstract>Terpenes are plant secondary metabolites known for their anti-inflammatory and antioxidant activities. According to ethnobotanical knowledge,
Sweet was used in traditional medicine against inflammation. The present study was conducted to determine the triterpene profile and antioxidant and anti-inflammatory activity of supercritical CO
(SC-CO
) extracts of
Sweet flower (RLF). An LC-APCI-MS/MS analysis showed the presence of eight pentacyclic triterpenes and one phytosterol in the extracts obtained with pure CO
as well as CO
with the addition of aqueous ethanol as a co-solvent. Among the compounds detected, oleanolic/ursolic acid, β-sitosterol and 3
-taraxerol were the most abundant. The extract obtained with pure SC-CO
was additionally subjected to HS-SPME-GC-FID-MS, which revealed more than 100 volatiles, mainly eugenol,
-phenylethanol, dodecane,
-caryophyllene, estragole and (
)- and (
)-cinnamyl alcohol, followed by
-cadinene. The extracts demonstrated significant hyaluronidase inhibition and exhibited varying modes of lipoxygenase and xanthine oxidase inhibitory activities. The studies of RLF have shown that their SC-CO
extracts can be a rich source of triterpenes with anti-inflammatory potential.</abstract><cop>Switzerland</cop><pmid>39337440</pmid><doi>10.3390/ijms25189952</doi><orcidid>https://orcid.org/0000-0003-4952-3147</orcidid><orcidid>https://orcid.org/0000-0003-1690-4090</orcidid><orcidid>https://orcid.org/0000-0002-2838-550X</orcidid><orcidid>https://orcid.org/0000-0002-4018-5260</orcidid><orcidid>https://orcid.org/0000-0002-0010-6089</orcidid><orcidid>https://orcid.org/0000-0002-7308-4335</orcidid></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; PubMed Central; EZB Electronic Journals Library |
| subjects | Anti-Inflammatory Agents - chemistry Anti-Inflammatory Agents - pharmacology Antioxidants - chemistry Antioxidants - pharmacology Carbon Dioxide - chemistry Chromatography, Supercritical Fluid - methods Flowers - chemistry Plant Extracts - chemistry Plant Extracts - pharmacology Rhododendron - chemistry Tandem Mass Spectrometry Terpenes - chemistry Terpenes - pharmacology |
| title | Rhododendron luteum Sweet Flower Supercritical CO 2 Extracts: Terpenes Composition, Pro-Inflammatory Enzymes Inhibition and Antioxidant Activity |
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