In-situ monitoring of cellular H 2 O 2 within 3D cell clusters using conductive scaffolds
Accurately monitoring H O concentrations in 3D cell clusters is challenging due to limited diffusion and rapid degradation of H O in the culture medium. Despite the incorporation of three-dimensional cell culture approaches, the detection technology has largely remained as a 2D planar system. In thi...
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| Veröffentlicht in: | Talanta (Oxford) 2024-11, Vol.279, p.126559 |
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| container_title | Talanta (Oxford) |
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| creator | Zhu, Ling Bai, Mingxia Xiao, Shenghao Liu, Yanhui Zhu, Qin Wang, Zixuan Zhao, Jiaqian Zhang, Wei Chen, Dajing |
| description | Accurately monitoring H
O
concentrations in 3D cell clusters is challenging due to limited diffusion and rapid degradation of H
O
in the culture medium. Despite the incorporation of three-dimensional cell culture approaches, the detection technology has largely remained as a 2D planar system. In this study, we present a versatile approach of 3D electrochemical sensing utilizing carbon nanotubes as conductive scaffolds for in-situ monitoring of H
O
in cell clusters. These scaffolds enabled direct contact between H
O
released from cells and the electrodes, thereby improving sensitivity and ensuring biocompatibility for cell aggregates. The scaffolds exhibited electrocatalytic behavior with a limit of detection of 6.7 nM H
O
. Additionally, the electrochemical responses of cell clusters with the scaffolds exhibited significantly higher current compared to clusters without scaffolds when stimulated with model drugs. This study underscores the potential of conductive scaffolds for real-time monitoring of H
O
released from cell clusters in 3D microenvironments. |
| format | Article |
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O
concentrations in 3D cell clusters is challenging due to limited diffusion and rapid degradation of H
O
in the culture medium. Despite the incorporation of three-dimensional cell culture approaches, the detection technology has largely remained as a 2D planar system. In this study, we present a versatile approach of 3D electrochemical sensing utilizing carbon nanotubes as conductive scaffolds for in-situ monitoring of H
O
in cell clusters. These scaffolds enabled direct contact between H
O
released from cells and the electrodes, thereby improving sensitivity and ensuring biocompatibility for cell aggregates. The scaffolds exhibited electrocatalytic behavior with a limit of detection of 6.7 nM H
O
. Additionally, the electrochemical responses of cell clusters with the scaffolds exhibited significantly higher current compared to clusters without scaffolds when stimulated with model drugs. This study underscores the potential of conductive scaffolds for real-time monitoring of H
O
released from cell clusters in 3D microenvironments.</description><identifier>EISSN: 1873-3573</identifier><identifier>PMID: 39018950</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Cell Culture Techniques, Three Dimensional - methods ; Electric Conductivity ; Electrochemical Techniques - methods ; Electrodes ; Humans ; Hydrogen Peroxide - chemistry ; Nanotubes, Carbon - chemistry ; Tissue Scaffolds - chemistry</subject><ispartof>Talanta (Oxford), 2024-11, Vol.279, p.126559</ispartof><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39018950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Ling</creatorcontrib><creatorcontrib>Bai, Mingxia</creatorcontrib><creatorcontrib>Xiao, Shenghao</creatorcontrib><creatorcontrib>Liu, Yanhui</creatorcontrib><creatorcontrib>Zhu, Qin</creatorcontrib><creatorcontrib>Wang, Zixuan</creatorcontrib><creatorcontrib>Zhao, Jiaqian</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Chen, Dajing</creatorcontrib><title>In-situ monitoring of cellular H 2 O 2 within 3D cell clusters using conductive scaffolds</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Accurately monitoring H
O
concentrations in 3D cell clusters is challenging due to limited diffusion and rapid degradation of H
O
in the culture medium. Despite the incorporation of three-dimensional cell culture approaches, the detection technology has largely remained as a 2D planar system. In this study, we present a versatile approach of 3D electrochemical sensing utilizing carbon nanotubes as conductive scaffolds for in-situ monitoring of H
O
in cell clusters. These scaffolds enabled direct contact between H
O
released from cells and the electrodes, thereby improving sensitivity and ensuring biocompatibility for cell aggregates. The scaffolds exhibited electrocatalytic behavior with a limit of detection of 6.7 nM H
O
. Additionally, the electrochemical responses of cell clusters with the scaffolds exhibited significantly higher current compared to clusters without scaffolds when stimulated with model drugs. This study underscores the potential of conductive scaffolds for real-time monitoring of H
O
released from cell clusters in 3D microenvironments.</description><subject>Cell Culture Techniques, Three Dimensional - methods</subject><subject>Electric Conductivity</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Humans</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjssKwjAURIMgtj5-Qe4PFNKG0nbtg7py48ZViWmqkTQpuYni3_tA1y6GWZw5MCMSp2XBEpYXLCJTxCulNGOUTUjEKpqWVU5jctyZBJUP0FujvHXKnMF2IKTWQXMHNWSwf-Wu_EUZYOsPAqEDeukQAr4NYU0bhFc3CSh411nd4pyMO65RLr49I8vt5rCqkyGcetk2g1M9d4_m94X9HTwBfUw-kA</recordid><startdate>20241101</startdate><enddate>20241101</enddate><creator>Zhu, Ling</creator><creator>Bai, Mingxia</creator><creator>Xiao, Shenghao</creator><creator>Liu, Yanhui</creator><creator>Zhu, Qin</creator><creator>Wang, Zixuan</creator><creator>Zhao, Jiaqian</creator><creator>Zhang, Wei</creator><creator>Chen, Dajing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20241101</creationdate><title>In-situ monitoring of cellular H 2 O 2 within 3D cell clusters using conductive scaffolds</title><author>Zhu, Ling ; Bai, Mingxia ; Xiao, Shenghao ; Liu, Yanhui ; Zhu, Qin ; Wang, Zixuan ; Zhao, Jiaqian ; Zhang, Wei ; Chen, Dajing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_390189503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cell Culture Techniques, Three Dimensional - methods</topic><topic>Electric Conductivity</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrodes</topic><topic>Humans</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Ling</creatorcontrib><creatorcontrib>Bai, Mingxia</creatorcontrib><creatorcontrib>Xiao, Shenghao</creatorcontrib><creatorcontrib>Liu, Yanhui</creatorcontrib><creatorcontrib>Zhu, Qin</creatorcontrib><creatorcontrib>Wang, Zixuan</creatorcontrib><creatorcontrib>Zhao, Jiaqian</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Chen, Dajing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Ling</au><au>Bai, Mingxia</au><au>Xiao, Shenghao</au><au>Liu, Yanhui</au><au>Zhu, Qin</au><au>Wang, Zixuan</au><au>Zhao, Jiaqian</au><au>Zhang, Wei</au><au>Chen, Dajing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-situ monitoring of cellular H 2 O 2 within 3D cell clusters using conductive scaffolds</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2024-11-01</date><risdate>2024</risdate><volume>279</volume><spage>126559</spage><pages>126559-</pages><eissn>1873-3573</eissn><abstract>Accurately monitoring H
O
concentrations in 3D cell clusters is challenging due to limited diffusion and rapid degradation of H
O
in the culture medium. Despite the incorporation of three-dimensional cell culture approaches, the detection technology has largely remained as a 2D planar system. In this study, we present a versatile approach of 3D electrochemical sensing utilizing carbon nanotubes as conductive scaffolds for in-situ monitoring of H
O
in cell clusters. These scaffolds enabled direct contact between H
O
released from cells and the electrodes, thereby improving sensitivity and ensuring biocompatibility for cell aggregates. The scaffolds exhibited electrocatalytic behavior with a limit of detection of 6.7 nM H
O
. Additionally, the electrochemical responses of cell clusters with the scaffolds exhibited significantly higher current compared to clusters without scaffolds when stimulated with model drugs. This study underscores the potential of conductive scaffolds for real-time monitoring of H
O
released from cell clusters in 3D microenvironments.</abstract><cop>Netherlands</cop><pmid>39018950</pmid></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1873-3573 |
| ispartof | Talanta (Oxford), 2024-11, Vol.279, p.126559 |
| issn | 1873-3573 |
| language | eng |
| recordid | cdi_pubmed_primary_39018950 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Cell Culture Techniques, Three Dimensional - methods Electric Conductivity Electrochemical Techniques - methods Electrodes Humans Hydrogen Peroxide - chemistry Nanotubes, Carbon - chemistry Tissue Scaffolds - chemistry |
| title | In-situ monitoring of cellular H 2 O 2 within 3D cell clusters using conductive scaffolds |
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