A high-fidelity DNAzyme-assisted CRISPR/Cas13a system with single-nucleotide resolved specificity

A CRISPR/Cas system represents an innovative tool for developing a new-generation biosensing and diagnostic strategy. However, the off-target issue ( i.e. , mistaken cleavage of nucleic acid targets and reporters) remains a great challenge for its practical applications. We hypothesize that this iss...

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Veröffentlicht in:	Chemical science (Cambridge) 2024-05, Vol.15 (18), p.6934-6942
Hauptverfasser:	Wu, Yunping, Jin, Ruigang, Chang, Yangyang, Liu, Meng
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Cleavage CRISPR Nucleic acids Nucleotides Ribonucleic acid RNA
Online-Zugang:	Volltext
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