Expression of the Mouse Serum Albumin Gene Introduced into Differentiated and Dedifferentiated Rat Hepatoma Cells
A 23-kilobase-pair segment of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5′and 4.3 kilobase pairs of 3′flanking sequences has been introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase cDNA is under the control of simian...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-02, Vol.82 (3), p.765-769 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Deschatrette, Jean Fougere-Deschatrette, Catherine Corcos, Laurent Schimke, Robert T. |
| description | A 23-kilobase-pair segment of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5′and 4.3 kilobase pairs of 3′flanking sequences has been introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase cDNA is under the control of simian virus 40 sequences. This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. In contrast, only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. These results show that a transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell. |
| doi_str_mv | 10.1073/pnas.82.3.765 |
| format | Article |
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This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. In contrast, only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. These results show that a transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.3.765</identifier><identifier>PMID: 3856230</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Albumins ; Animals ; Bacteriophages - genetics ; Biological and medical sciences ; Cell Differentiation ; Cell Line ; Cell lines ; Cells ; Cellular differentiation ; Complementary DNA ; Drug Resistance ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation ; Hepatocellular carcinoma ; Liver Neoplasms, Experimental - drug therapy ; Liver Neoplasms, Experimental - metabolism ; Messenger RNA ; Methotrexate - therapeutic use ; Molecular and cellular biology ; Molecular genetics ; Plasmids ; Rats ; RNA, Messenger - analysis ; Secretion ; Serum Albumin - genetics ; Tetrahydrofolate Dehydrogenase - genetics ; Transfection</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-02, Vol.82 (3), p.765-769</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-2ee6398ac0dce1eae2f949583854b2d4e115f92a1253e847ac94a22c470816943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/3.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25352$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25352$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,725,778,782,801,883,27913,27914,53780,53782,58006,58239</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9108015$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3856230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deschatrette, Jean</creatorcontrib><creatorcontrib>Fougere-Deschatrette, Catherine</creatorcontrib><creatorcontrib>Corcos, Laurent</creatorcontrib><creatorcontrib>Schimke, Robert T.</creatorcontrib><title>Expression of the Mouse Serum Albumin Gene Introduced into Differentiated and Dedifferentiated Rat Hepatoma Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A 23-kilobase-pair segment of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5′and 4.3 kilobase pairs of 3′flanking sequences has been introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase cDNA is under the control of simian virus 40 sequences. This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. In contrast, only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. These results show that a transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell.</description><subject>Albumins</subject><subject>Animals</subject><subject>Bacteriophages - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cellular differentiation</subject><subject>Complementary DNA</subject><subject>Drug Resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Hepatocellular carcinoma</subject><subject>Liver Neoplasms, Experimental - drug therapy</subject><subject>Liver Neoplasms, Experimental - metabolism</subject><subject>Messenger RNA</subject><subject>Methotrexate - therapeutic use</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Plasmids</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>Secretion</subject><subject>Serum Albumin - genetics</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><subject>Transfection</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhyAWB5ANwy-KvxPGBQ7UtbaUiJD7OlteZUFeJndoOKv89Xm1YtRc4WZr3m-c3Mwi9pGRNieQfJm_SumVrvpZN_QitKFG0aoQij9GKECarVjDxFD1L6YYQouqWHKEj3tYN42SFbs_upggpueBx6HG-Bvw5zAnwN4jziE-G7Tw6j8_BA770OYZuttBh53PAp67vIYLPzuRSM77Dp9A9LH41GV_AZHIYDd7AMKTn6ElvhgQvlvcY_fh09n1zUV19Ob_cnFxVtqY0Vwyg4ao1lnQWKBhgvRIlfUkutqwTQGndK2Yoqzm0QhqrhGHMCkla2ijBj9HHve80b0coJiW9GfQU3Wjibx2M0w8V7671z_BLcyUpk6X__dIfw-0MKevRJVsmMB7KhrRsCKsl5_8FqWCMi4YWsNqDNoaUIvSHMJTo3S317pa6ZZoX97rwb-5PcKCX4xX97aKbZM3QR-OtSwdMUdISurN5vWA797_qvV_e_UPW_TwMGe5y4V7tuZuUQzyAZf01438AGvbIyw</recordid><startdate>19850201</startdate><enddate>19850201</enddate><creator>Deschatrette, Jean</creator><creator>Fougere-Deschatrette, Catherine</creator><creator>Corcos, Laurent</creator><creator>Schimke, Robert T.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850201</creationdate><title>Expression of the Mouse Serum Albumin Gene Introduced into Differentiated and Dedifferentiated Rat Hepatoma Cells</title><author>Deschatrette, Jean ; Fougere-Deschatrette, Catherine ; Corcos, Laurent ; Schimke, Robert T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-2ee6398ac0dce1eae2f949583854b2d4e115f92a1253e847ac94a22c470816943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Albumins</topic><topic>Animals</topic><topic>Bacteriophages - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cells</topic><topic>Cellular differentiation</topic><topic>Complementary DNA</topic><topic>Drug Resistance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Hepatocellular carcinoma</topic><topic>Liver Neoplasms, Experimental - drug therapy</topic><topic>Liver Neoplasms, Experimental - metabolism</topic><topic>Messenger RNA</topic><topic>Methotrexate - therapeutic use</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Plasmids</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>Secretion</topic><topic>Serum Albumin - genetics</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deschatrette, Jean</creatorcontrib><creatorcontrib>Fougere-Deschatrette, Catherine</creatorcontrib><creatorcontrib>Corcos, Laurent</creatorcontrib><creatorcontrib>Schimke, Robert T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deschatrette, Jean</au><au>Fougere-Deschatrette, Catherine</au><au>Corcos, Laurent</au><au>Schimke, Robert T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the Mouse Serum Albumin Gene Introduced into Differentiated and Dedifferentiated Rat Hepatoma Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-02-01</date><risdate>1985</risdate><volume>82</volume><issue>3</issue><spage>765</spage><epage>769</epage><pages>765-769</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A 23-kilobase-pair segment of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5′and 4.3 kilobase pairs of 3′flanking sequences has been introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase cDNA is under the control of simian virus 40 sequences. This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. In contrast, only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. These results show that a transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3856230</pmid><doi>10.1073/pnas.82.3.765</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-02, Vol.82 (3), p.765-769 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Albumins Animals Bacteriophages - genetics Biological and medical sciences Cell Differentiation Cell Line Cell lines Cells Cellular differentiation Complementary DNA Drug Resistance Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Hepatocellular carcinoma Liver Neoplasms, Experimental - drug therapy Liver Neoplasms, Experimental - metabolism Messenger RNA Methotrexate - therapeutic use Molecular and cellular biology Molecular genetics Plasmids Rats RNA, Messenger - analysis Secretion Serum Albumin - genetics Tetrahydrofolate Dehydrogenase - genetics Transfection |
| title | Expression of the Mouse Serum Albumin Gene Introduced into Differentiated and Dedifferentiated Rat Hepatoma Cells |
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