Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples
Strongyloides myopotami is an intestinal nematode parasite of nutrias. Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecu...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2024, Vol.86(3), pp.349-353 |
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| creator | MORI, Yuga NAKA, Atsushi KODA, Ryosuke ISHIZUKA, Yuzuru HINENOYA, Atsushi SHIBAHARA, Tomoyuki SASAI, Kazumi MATSUBAYASHI, Makoto |
| description | Strongyloides myopotami is an intestinal nematode parasite of nutrias. Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecular identification using a few grams of the feces. Here, we attempted to identify S. myopotami using DNA extracted from eggs obtained from fecal samples. Among previously reported primer pairs targeting the 18S rRNA gene of Strongyloides spp., most could not be successful. We newly designed primers that successfully amplified the partial sequences in S. myopotami, resulting in being sequenced. Our simple protocol can be useful in nationwide surveys for clarifying the risk of human infection. |
| doi_str_mv | 10.1292/jvms.23-0198 |
| format | Article |
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Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecular identification using a few grams of the feces. Here, we attempted to identify S. myopotami using DNA extracted from eggs obtained from fecal samples. Among previously reported primer pairs targeting the 18S rRNA gene of Strongyloides spp., most could not be successful. We newly designed primers that successfully amplified the partial sequences in S. myopotami, resulting in being sequenced. Our simple protocol can be useful in nationwide surveys for clarifying the risk of human infection.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.23-0198</identifier><identifier>PMID: 38281761</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; egg DNA ; Feces ; Feces - parasitology ; Humans ; Intestinal Diseases, Parasitic - parasitology ; Intestinal Diseases, Parasitic - veterinary ; Myocastor ; nutria ; Ovum ; PCR ; Physical characteristics ; Rodent Diseases - parasitology ; Rodentia ; rRNA 18S ; Strongyloides ; Strongyloides - genetics ; Strongyloides myopotami ; Wildlife Science</subject><ispartof>Journal of Veterinary Medical Science, 2024, Vol.86(3), pp.349-353</ispartof><rights>2024 by the Japanese Society of Veterinary Science</rights><rights>2024. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). 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Vet. Med. Sci.</addtitle><description>Strongyloides myopotami is an intestinal nematode parasite of nutrias. Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecular identification using a few grams of the feces. Here, we attempted to identify S. myopotami using DNA extracted from eggs obtained from fecal samples. Among previously reported primer pairs targeting the 18S rRNA gene of Strongyloides spp., most could not be successful. We newly designed primers that successfully amplified the partial sequences in S. myopotami, resulting in being sequenced. Our simple protocol can be useful in nationwide surveys for clarifying the risk of human infection.</description><subject>Animals</subject><subject>egg DNA</subject><subject>Feces</subject><subject>Feces - parasitology</subject><subject>Humans</subject><subject>Intestinal Diseases, Parasitic - parasitology</subject><subject>Intestinal Diseases, Parasitic - veterinary</subject><subject>Myocastor</subject><subject>nutria</subject><subject>Ovum</subject><subject>PCR</subject><subject>Physical characteristics</subject><subject>Rodent Diseases - parasitology</subject><subject>Rodentia</subject><subject>rRNA 18S</subject><subject>Strongyloides</subject><subject>Strongyloides - genetics</subject><subject>Strongyloides myopotami</subject><subject>Wildlife Science</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxS0EotvCjTOyxIVDUzx2HMcnVCo-KlXiAJw4WI7jbL1y7GA7K-1_T6JdVsBl5vB-evNGD6FXQG6ASvputx_zDWUVAdk-QRtgtahEzeRTtCESmkpQTi7QZc47QijUjXyOLlhLWxANbNDP-3FKcW97PEZvzex1wq63objBGV1cDDgO-FtJMWwPPi5SxuMhTrHo0WEXcJhLcjrjObuwxYM12uOsx8nb_AI9G7TP9uVpX6Efnz5-v_tSPXz9fH93-1AZzmmptOiA962xRGhjeNfX1DDRC8IFGNkZwQ0RnHFpac87ZhtNWM8aMAJqgJayK_T-6DvN3Wh7s6RP2qspuVGng4raqX-V4B7VNu4VENkw0srF4e3JIcVfs81FjS4b670ONs5ZUQlS1AKa9dib_9BdnFNY_lOMsJatUeuFuj5SJsWckx3OaYCotTa11qYoU2ttC_767w_O8J-eFuDDEdjlorf2DOhUnPH26NY2iq3j5HoWzaNOygb2G6Qnrag</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>MORI, Yuga</creator><creator>NAKA, Atsushi</creator><creator>KODA, Ryosuke</creator><creator>ISHIZUKA, Yuzuru</creator><creator>HINENOYA, Atsushi</creator><creator>SHIBAHARA, Tomoyuki</creator><creator>SASAI, Kazumi</creator><creator>MATSUBAYASHI, Makoto</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2024</creationdate><title>Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples</title><author>MORI, Yuga ; NAKA, Atsushi ; KODA, Ryosuke ; ISHIZUKA, Yuzuru ; HINENOYA, Atsushi ; SHIBAHARA, Tomoyuki ; SASAI, Kazumi ; MATSUBAYASHI, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-a7b15d8ce07acc5bd42c37d70571c9bc75c075359e2d5b3e6a03d361c71411823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>egg DNA</topic><topic>Feces</topic><topic>Feces - parasitology</topic><topic>Humans</topic><topic>Intestinal Diseases, Parasitic - parasitology</topic><topic>Intestinal Diseases, Parasitic - veterinary</topic><topic>Myocastor</topic><topic>nutria</topic><topic>Ovum</topic><topic>PCR</topic><topic>Physical characteristics</topic><topic>Rodent Diseases - parasitology</topic><topic>Rodentia</topic><topic>rRNA 18S</topic><topic>Strongyloides</topic><topic>Strongyloides - genetics</topic><topic>Strongyloides myopotami</topic><topic>Wildlife Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MORI, Yuga</creatorcontrib><creatorcontrib>NAKA, Atsushi</creatorcontrib><creatorcontrib>KODA, Ryosuke</creatorcontrib><creatorcontrib>ISHIZUKA, Yuzuru</creatorcontrib><creatorcontrib>HINENOYA, Atsushi</creatorcontrib><creatorcontrib>SHIBAHARA, Tomoyuki</creatorcontrib><creatorcontrib>SASAI, Kazumi</creatorcontrib><creatorcontrib>MATSUBAYASHI, Makoto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MORI, Yuga</au><au>NAKA, Atsushi</au><au>KODA, Ryosuke</au><au>ISHIZUKA, Yuzuru</au><au>HINENOYA, Atsushi</au><au>SHIBAHARA, Tomoyuki</au><au>SASAI, Kazumi</au><au>MATSUBAYASHI, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. 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| subjects | Animals egg DNA Feces Feces - parasitology Humans Intestinal Diseases, Parasitic - parasitology Intestinal Diseases, Parasitic - veterinary Myocastor nutria Ovum PCR Physical characteristics Rodent Diseases - parasitology Rodentia rRNA 18S Strongyloides Strongyloides - genetics Strongyloides myopotami Wildlife Science |
| title | Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples |
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