Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction
Here, mitochondria were isolated from mesenchymal stem cells (MSCs) after being treated with mitochondria-stimulating substrates, 50 μM metformin (Met), and 40 μM dichloroacetic acid (DCA). The isolated mitochondria (2 × 10 particles) were characterized and encapsulated inside 100 μl hydrogel compos...
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| Veröffentlicht in: | International journal of biological macromolecules 2024-01, p.129633 |
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| creator | Hassanpour, Parisa Sadeghsoltani, Fatemeh Haiaty, Sanya Zakeri, Ziba Saghebasl, Solmaz Izadpanah, Melika Boroumand, Safieh Mota, Ali Rahmati, Mohammad Rahbarghazi, Reza Talebi, Mehdi Rabbani, Shahram Tafti, Seyed Hossein Ahmadi |
| description | Here, mitochondria were isolated from mesenchymal stem cells (MSCs) after being treated with mitochondria-stimulating substrates, 50 μM metformin (Met), and 40 μM dichloroacetic acid (DCA). The isolated mitochondria (2 × 10
particles) were characterized and encapsulated inside 100 μl hydrogel composed of alginate (3 % w/v; Alg)/gelatin (Gel; 1 % w/v) enriched with 1 μM pyrrole (Pyr) solidified in the presence of 0.2 M FeCl
. The physicochemical properties and cytocompatibility of prepared hydrogels were assessed using FTIR, swelling, biodegradation, porosity assays, and scanning electron microscopy (SEM). The mitochondria-bearing hydrogel was injected into the ischemic area of rat hearts. FTIR absorption bands represented that the addition of FeCl
led to polypyrrole (PPy) formation, polysaccharide oxidation, and interaction between Alg and Gel. SEM images exhibited porous structure and the size of pores was reduced in Alg/Gel + PPy group compared to Alg + PPy hydrogel. Based on the data, both Alg + PPy and Alg/Gel + PPy hydrogels can preserve the integrity and morphology of loaded mitochondria. It was noted that Alg/Gel + PPy hydrogel possessed a higher swelling ratio, degradation, and porosity compared to Alg + PPy group. Data confirmed that Alg/Gel + PPy hydrogel containing 1 μM Pyr yielded the highest survival rate compared to groups with 2 and 4 μM Pyr (p |
| doi_str_mv | 10.1016/j.ijbiomac.2024.129633 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_38253146</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>38253146</sourcerecordid><originalsourceid>FETCH-pubmed_primary_382531463</originalsourceid><addsrcrecordid>eNqFj8tOwzAQRS0kRMvjF6r5gQQ7bkO6RiA27NhXE3uSTORHZSdI_Qs-GS-ALauZqznnSiPETslaSdU-zjXPPUePpm5ks69Vc2y1vhJb1T0dKyml3ojbnOeytQfV3YiN7pqDVvt2K77eeYlmisEmxspFtGQB3cgBF6p6zCVOF5viSA7QGHKUysXCMhFMhI7DCHEAf4kGk-XVwycjYBi5KIEyZ-AACMUCH21pKTSadaE_B11BBkxm4RjuxfWALtPDz7wTu9eXj-e36rz2nuzpnNhjupx-X9D_At-VP1yW</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction</title><source>Elsevier ScienceDirect Journals</source><creator>Hassanpour, Parisa ; Sadeghsoltani, Fatemeh ; Haiaty, Sanya ; Zakeri, Ziba ; Saghebasl, Solmaz ; Izadpanah, Melika ; Boroumand, Safieh ; Mota, Ali ; Rahmati, Mohammad ; Rahbarghazi, Reza ; Talebi, Mehdi ; Rabbani, Shahram ; Tafti, Seyed Hossein Ahmadi</creator><creatorcontrib>Hassanpour, Parisa ; Sadeghsoltani, Fatemeh ; Haiaty, Sanya ; Zakeri, Ziba ; Saghebasl, Solmaz ; Izadpanah, Melika ; Boroumand, Safieh ; Mota, Ali ; Rahmati, Mohammad ; Rahbarghazi, Reza ; Talebi, Mehdi ; Rabbani, Shahram ; Tafti, Seyed Hossein Ahmadi</creatorcontrib><description>Here, mitochondria were isolated from mesenchymal stem cells (MSCs) after being treated with mitochondria-stimulating substrates, 50 μM metformin (Met), and 40 μM dichloroacetic acid (DCA). The isolated mitochondria (2 × 10
particles) were characterized and encapsulated inside 100 μl hydrogel composed of alginate (3 % w/v; Alg)/gelatin (Gel; 1 % w/v) enriched with 1 μM pyrrole (Pyr) solidified in the presence of 0.2 M FeCl
. The physicochemical properties and cytocompatibility of prepared hydrogels were assessed using FTIR, swelling, biodegradation, porosity assays, and scanning electron microscopy (SEM). The mitochondria-bearing hydrogel was injected into the ischemic area of rat hearts. FTIR absorption bands represented that the addition of FeCl
led to polypyrrole (PPy) formation, polysaccharide oxidation, and interaction between Alg and Gel. SEM images exhibited porous structure and the size of pores was reduced in Alg/Gel + PPy group compared to Alg + PPy hydrogel. Based on the data, both Alg + PPy and Alg/Gel + PPy hydrogels can preserve the integrity and morphology of loaded mitochondria. It was noted that Alg/Gel + PPy hydrogel possessed a higher swelling ratio, degradation, and porosity compared to Alg + PPy group. Data confirmed that Alg/Gel + PPy hydrogel containing 1 μM Pyr yielded the highest survival rate compared to groups with 2 and 4 μM Pyr (p < 0.05). Injection of mitochondria-loaded Alg/Gel + PPy hydrogel yielded significant restoration of left ventricle thickness compared to the infarction, mitochondria, and Alg/Gel + PPy hydrogel groups 14 days post-injection (p < 0.05). Histological analyses revealed a significant increase of vWF
capillaries and α-SMA
arterioles in the mitochondria-loaded Alg/Gel + PPy hydrogel group (p < 0.05). Immunofluorescence imaging revealed the ability of rat cardiomyocytes to uptake mitochondria alone or after being loaded into Alg/Gel + PPy hydrogel. These effects were evident in the Alg/Gel + PPy group. Taken together, electroconductive Alg-based hydrogels are suitable platforms for the transplantation of cells and organelles and the regeneration of ischemic heart changes.</description><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.129633</identifier><identifier>PMID: 38253146</identifier><language>eng</language><publisher>Netherlands</publisher><ispartof>International journal of biological macromolecules, 2024-01, p.129633</ispartof><rights>Copyright © 2024. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38253146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassanpour, Parisa</creatorcontrib><creatorcontrib>Sadeghsoltani, Fatemeh</creatorcontrib><creatorcontrib>Haiaty, Sanya</creatorcontrib><creatorcontrib>Zakeri, Ziba</creatorcontrib><creatorcontrib>Saghebasl, Solmaz</creatorcontrib><creatorcontrib>Izadpanah, Melika</creatorcontrib><creatorcontrib>Boroumand, Safieh</creatorcontrib><creatorcontrib>Mota, Ali</creatorcontrib><creatorcontrib>Rahmati, Mohammad</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Talebi, Mehdi</creatorcontrib><creatorcontrib>Rabbani, Shahram</creatorcontrib><creatorcontrib>Tafti, Seyed Hossein Ahmadi</creatorcontrib><title>Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Here, mitochondria were isolated from mesenchymal stem cells (MSCs) after being treated with mitochondria-stimulating substrates, 50 μM metformin (Met), and 40 μM dichloroacetic acid (DCA). The isolated mitochondria (2 × 10
particles) were characterized and encapsulated inside 100 μl hydrogel composed of alginate (3 % w/v; Alg)/gelatin (Gel; 1 % w/v) enriched with 1 μM pyrrole (Pyr) solidified in the presence of 0.2 M FeCl
. The physicochemical properties and cytocompatibility of prepared hydrogels were assessed using FTIR, swelling, biodegradation, porosity assays, and scanning electron microscopy (SEM). The mitochondria-bearing hydrogel was injected into the ischemic area of rat hearts. FTIR absorption bands represented that the addition of FeCl
led to polypyrrole (PPy) formation, polysaccharide oxidation, and interaction between Alg and Gel. SEM images exhibited porous structure and the size of pores was reduced in Alg/Gel + PPy group compared to Alg + PPy hydrogel. Based on the data, both Alg + PPy and Alg/Gel + PPy hydrogels can preserve the integrity and morphology of loaded mitochondria. It was noted that Alg/Gel + PPy hydrogel possessed a higher swelling ratio, degradation, and porosity compared to Alg + PPy group. Data confirmed that Alg/Gel + PPy hydrogel containing 1 μM Pyr yielded the highest survival rate compared to groups with 2 and 4 μM Pyr (p < 0.05). Injection of mitochondria-loaded Alg/Gel + PPy hydrogel yielded significant restoration of left ventricle thickness compared to the infarction, mitochondria, and Alg/Gel + PPy hydrogel groups 14 days post-injection (p < 0.05). Histological analyses revealed a significant increase of vWF
capillaries and α-SMA
arterioles in the mitochondria-loaded Alg/Gel + PPy hydrogel group (p < 0.05). Immunofluorescence imaging revealed the ability of rat cardiomyocytes to uptake mitochondria alone or after being loaded into Alg/Gel + PPy hydrogel. These effects were evident in the Alg/Gel + PPy group. Taken together, electroconductive Alg-based hydrogels are suitable platforms for the transplantation of cells and organelles and the regeneration of ischemic heart changes.</description><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFj8tOwzAQRS0kRMvjF6r5gQQ7bkO6RiA27NhXE3uSTORHZSdI_Qs-GS-ALauZqznnSiPETslaSdU-zjXPPUePpm5ks69Vc2y1vhJb1T0dKyml3ojbnOeytQfV3YiN7pqDVvt2K77eeYlmisEmxspFtGQB3cgBF6p6zCVOF5viSA7QGHKUysXCMhFMhI7DCHEAf4kGk-XVwycjYBi5KIEyZ-AACMUCH21pKTSadaE_B11BBkxm4RjuxfWALtPDz7wTu9eXj-e36rz2nuzpnNhjupx-X9D_At-VP1yW</recordid><startdate>20240120</startdate><enddate>20240120</enddate><creator>Hassanpour, Parisa</creator><creator>Sadeghsoltani, Fatemeh</creator><creator>Haiaty, Sanya</creator><creator>Zakeri, Ziba</creator><creator>Saghebasl, Solmaz</creator><creator>Izadpanah, Melika</creator><creator>Boroumand, Safieh</creator><creator>Mota, Ali</creator><creator>Rahmati, Mohammad</creator><creator>Rahbarghazi, Reza</creator><creator>Talebi, Mehdi</creator><creator>Rabbani, Shahram</creator><creator>Tafti, Seyed Hossein Ahmadi</creator><scope>NPM</scope></search><sort><creationdate>20240120</creationdate><title>Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction</title><author>Hassanpour, Parisa ; Sadeghsoltani, Fatemeh ; Haiaty, Sanya ; Zakeri, Ziba ; Saghebasl, Solmaz ; Izadpanah, Melika ; Boroumand, Safieh ; Mota, Ali ; Rahmati, Mohammad ; Rahbarghazi, Reza ; Talebi, Mehdi ; Rabbani, Shahram ; Tafti, Seyed Hossein Ahmadi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_382531463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassanpour, Parisa</creatorcontrib><creatorcontrib>Sadeghsoltani, Fatemeh</creatorcontrib><creatorcontrib>Haiaty, Sanya</creatorcontrib><creatorcontrib>Zakeri, Ziba</creatorcontrib><creatorcontrib>Saghebasl, Solmaz</creatorcontrib><creatorcontrib>Izadpanah, Melika</creatorcontrib><creatorcontrib>Boroumand, Safieh</creatorcontrib><creatorcontrib>Mota, Ali</creatorcontrib><creatorcontrib>Rahmati, Mohammad</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Talebi, Mehdi</creatorcontrib><creatorcontrib>Rabbani, Shahram</creatorcontrib><creatorcontrib>Tafti, Seyed Hossein Ahmadi</creatorcontrib><collection>PubMed</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassanpour, Parisa</au><au>Sadeghsoltani, Fatemeh</au><au>Haiaty, Sanya</au><au>Zakeri, Ziba</au><au>Saghebasl, Solmaz</au><au>Izadpanah, Melika</au><au>Boroumand, Safieh</au><au>Mota, Ali</au><au>Rahmati, Mohammad</au><au>Rahbarghazi, Reza</au><au>Talebi, Mehdi</au><au>Rabbani, Shahram</au><au>Tafti, Seyed Hossein Ahmadi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-01-20</date><risdate>2024</risdate><spage>129633</spage><pages>129633-</pages><eissn>1879-0003</eissn><abstract>Here, mitochondria were isolated from mesenchymal stem cells (MSCs) after being treated with mitochondria-stimulating substrates, 50 μM metformin (Met), and 40 μM dichloroacetic acid (DCA). The isolated mitochondria (2 × 10
particles) were characterized and encapsulated inside 100 μl hydrogel composed of alginate (3 % w/v; Alg)/gelatin (Gel; 1 % w/v) enriched with 1 μM pyrrole (Pyr) solidified in the presence of 0.2 M FeCl
. The physicochemical properties and cytocompatibility of prepared hydrogels were assessed using FTIR, swelling, biodegradation, porosity assays, and scanning electron microscopy (SEM). The mitochondria-bearing hydrogel was injected into the ischemic area of rat hearts. FTIR absorption bands represented that the addition of FeCl
led to polypyrrole (PPy) formation, polysaccharide oxidation, and interaction between Alg and Gel. SEM images exhibited porous structure and the size of pores was reduced in Alg/Gel + PPy group compared to Alg + PPy hydrogel. Based on the data, both Alg + PPy and Alg/Gel + PPy hydrogels can preserve the integrity and morphology of loaded mitochondria. It was noted that Alg/Gel + PPy hydrogel possessed a higher swelling ratio, degradation, and porosity compared to Alg + PPy group. Data confirmed that Alg/Gel + PPy hydrogel containing 1 μM Pyr yielded the highest survival rate compared to groups with 2 and 4 μM Pyr (p < 0.05). Injection of mitochondria-loaded Alg/Gel + PPy hydrogel yielded significant restoration of left ventricle thickness compared to the infarction, mitochondria, and Alg/Gel + PPy hydrogel groups 14 days post-injection (p < 0.05). Histological analyses revealed a significant increase of vWF
capillaries and α-SMA
arterioles in the mitochondria-loaded Alg/Gel + PPy hydrogel group (p < 0.05). Immunofluorescence imaging revealed the ability of rat cardiomyocytes to uptake mitochondria alone or after being loaded into Alg/Gel + PPy hydrogel. These effects were evident in the Alg/Gel + PPy group. Taken together, electroconductive Alg-based hydrogels are suitable platforms for the transplantation of cells and organelles and the regeneration of ischemic heart changes.</abstract><cop>Netherlands</cop><pmid>38253146</pmid><doi>10.1016/j.ijbiomac.2024.129633</doi></addata></record> |
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| title | Mitochondria-loaded alginate-based hydrogel accelerated the healing of myocardium via angiogenesis in a rat model of acute myocardial infarction |
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